Lactobacillus agilis DSM 20509

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus agilis

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2AHN5|A0A0R2AHN5_9LACO Mannitol-1-phosphate 5-dehydrogenase OS=Lactobacillus agilis DSM 20509 OX=1423718 GN=mtlD PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.93EE3 pKa = 4.18GYY5 pKa = 10.53FMATAKK11 pKa = 10.77VIFATITGNNEE22 pKa = 4.0DD23 pKa = 3.69IADD26 pKa = 4.51IIAEE30 pKa = 3.97EE31 pKa = 4.65LEE33 pKa = 4.26NKK35 pKa = 10.2GLDD38 pKa = 3.58VEE40 pKa = 4.47VEE42 pKa = 4.6EE43 pKa = 5.74ISQADD48 pKa = 3.75PAEE51 pKa = 4.41LEE53 pKa = 4.45AVDD56 pKa = 4.5LCVICPYY63 pKa = 10.39TYY65 pKa = 10.84DD66 pKa = 3.66EE67 pKa = 5.23GSLPDD72 pKa = 4.01EE73 pKa = 4.84GMDD76 pKa = 5.07FYY78 pKa = 11.82DD79 pKa = 4.07EE80 pKa = 4.59LADD83 pKa = 3.5VDD85 pKa = 5.01LSGKK89 pKa = 10.04VYY91 pKa = 10.46GVAGSGDD98 pKa = 3.73TFYY101 pKa = 11.83GEE103 pKa = 4.48YY104 pKa = 10.45FCTAVDD110 pKa = 4.17LFGEE114 pKa = 4.47ALAKK118 pKa = 10.64AGASKK123 pKa = 10.8GADD126 pKa = 2.74NVKK129 pKa = 10.37INLAPDD135 pKa = 3.51EE136 pKa = 5.16DD137 pKa = 4.19EE138 pKa = 5.91DD139 pKa = 3.89IARR142 pKa = 11.84LEE144 pKa = 4.11EE145 pKa = 4.47FADD148 pKa = 3.71QLVKK152 pKa = 10.94ALGEE156 pKa = 4.05

Molecular weight: 16.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2AHG3|A0A0R2AHG3_9LACO Alkaline shock protein OS=Lactobacillus agilis DSM 20509 OX=1423718 GN=FC14_GL000112 PE=3 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84TNQNSLRR9 pKa = 11.84AIVLTGLFAAIIYY22 pKa = 9.93IGIWVLRR29 pKa = 11.84IPIPAMVGRR38 pKa = 11.84PFIHH42 pKa = 7.03FGNTLTAVAILTLGLRR58 pKa = 11.84NGMLAGIIGLGGFDD72 pKa = 3.98ILNGYY77 pKa = 8.89AATSWLTMLEE87 pKa = 4.24VVIVALVISGLFKK100 pKa = 10.91AFNYY104 pKa = 9.69QDD106 pKa = 3.02SRR108 pKa = 11.84RR109 pKa = 11.84NIIILAIGAGVTKK122 pKa = 10.48IFTSYY127 pKa = 9.09CTSIVEE133 pKa = 4.16ALMAGTVFKK142 pKa = 10.81VALVASFFSLPATVINSISTAICTPLLYY170 pKa = 10.66FFLKK174 pKa = 10.62RR175 pKa = 11.84ILQAVTKK182 pKa = 10.21RR183 pKa = 3.44

Molecular weight: 19.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1951

0

1951

598424

49

1549

306.7

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.79 ± 0.059

0.642 ± 0.014

5.369 ± 0.047

5.979 ± 0.056

4.193 ± 0.046

6.587 ± 0.053

1.782 ± 0.021

6.395 ± 0.044

7.24 ± 0.053

10.688 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.026

4.559 ± 0.04

3.393 ± 0.027

4.615 ± 0.048

3.921 ± 0.04

5.695 ± 0.044

5.71 ± 0.04

7.125 ± 0.048

0.963 ± 0.019

3.916 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski