Wuhan arthropod virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF51|A0A1L3KF51_9VIRU Uncharacterized protein OS=Wuhan arthropod virus 4 OX=1923693 PE=4 SV=1
MM1 pKa = 7.48SAEE4 pKa = 4.0QVQTPVNFVLQAPAGAFAAPPPAEE28 pKa = 4.23SHH30 pKa = 5.19WLVRR34 pKa = 11.84AYY36 pKa = 9.79KK37 pKa = 10.31RR38 pKa = 11.84LGSLWPLFLASWWFSIPNLMQVLVGAYY65 pKa = 9.36FDD67 pKa = 3.74KK68 pKa = 11.24LGWLVDD74 pKa = 3.87LYY76 pKa = 11.69GRR78 pKa = 11.84VEE80 pKa = 3.87YY81 pKa = 10.89SADD84 pKa = 3.13KK85 pKa = 10.62GIQYY89 pKa = 10.18VVDD92 pKa = 3.54YY93 pKa = 10.08RR94 pKa = 11.84LPLILGTCTTLIWACATAMMWKK116 pKa = 9.33WMNDD120 pKa = 3.44DD121 pKa = 3.69VKK123 pKa = 10.97DD124 pKa = 3.84TIDD127 pKa = 4.11RR128 pKa = 11.84EE129 pKa = 4.45VQVRR133 pKa = 11.84LLQEE137 pKa = 4.27GLAKK141 pKa = 10.1QAATQTVTDD150 pKa = 4.15PVEE153 pKa = 4.01FTPEE157 pKa = 3.74KK158 pKa = 10.33VKK160 pKa = 10.67PSSILIKK167 pKa = 9.72PEE169 pKa = 3.78RR170 pKa = 11.84PTFQAAIYY178 pKa = 10.63GEE180 pKa = 4.96LNGAWVRR187 pKa = 11.84SGQAFKK193 pKa = 10.97VRR195 pKa = 11.84HH196 pKa = 4.69QLITAYY202 pKa = 10.09HH203 pKa = 5.03VVEE206 pKa = 4.18SFQRR210 pKa = 11.84LRR212 pKa = 11.84LVTSSGEE219 pKa = 3.2IDD221 pKa = 3.29MASEE225 pKa = 4.28RR226 pKa = 11.84FRR228 pKa = 11.84WVEE231 pKa = 3.65GDD233 pKa = 3.68VAFCLLTPQEE243 pKa = 4.14NQILALTEE251 pKa = 4.03AKK253 pKa = 10.15LANHH257 pKa = 6.57EE258 pKa = 4.19FASNSGLIVKK268 pKa = 8.38ATGFNNSSYY277 pKa = 11.14GFLKK281 pKa = 10.22EE282 pKa = 4.05YY283 pKa = 10.09PAFGYY288 pKa = 10.23VEE290 pKa = 4.4YY291 pKa = 10.74AGSTVPGFSGCPYY304 pKa = 9.39VVGPKK309 pKa = 10.08VYY311 pKa = 10.27GMHH314 pKa = 7.11LGGGVEE320 pKa = 4.03NLGLNGAYY328 pKa = 10.46LDD330 pKa = 3.56MLVRR334 pKa = 11.84RR335 pKa = 11.84KK336 pKa = 10.42DD337 pKa = 3.65EE338 pKa = 3.96SSEE341 pKa = 4.31DD342 pKa = 3.59YY343 pKa = 11.29LFGQIEE349 pKa = 4.08KK350 pKa = 10.31HH351 pKa = 5.7EE352 pKa = 4.65KK353 pKa = 10.04YY354 pKa = 10.4DD355 pKa = 4.05YY356 pKa = 10.46QQSPYY361 pKa = 11.09DD362 pKa = 3.67PDD364 pKa = 3.45EE365 pKa = 4.05FRR367 pKa = 11.84LRR369 pKa = 11.84IGGKK373 pKa = 10.13YY374 pKa = 9.87YY375 pKa = 10.86LIDD378 pKa = 4.59ADD380 pKa = 4.17MLHH383 pKa = 7.15SLEE386 pKa = 4.28TRR388 pKa = 11.84QKK390 pKa = 9.67GAGSNKK396 pKa = 9.6VMFKK400 pKa = 10.6DD401 pKa = 4.01LEE403 pKa = 4.32YY404 pKa = 10.91DD405 pKa = 3.39AEE407 pKa = 4.77AYY409 pKa = 7.91EE410 pKa = 4.28YY411 pKa = 8.51PTEE414 pKa = 4.2EE415 pKa = 3.67EE416 pKa = 4.76HH417 pKa = 7.56IPVYY421 pKa = 10.11KK422 pKa = 10.27PKK424 pKa = 10.1EE425 pKa = 3.7IPKK428 pKa = 9.8IDD430 pKa = 3.88DD431 pKa = 4.31EE432 pKa = 4.35IGKK435 pKa = 9.68VSKK438 pKa = 10.58EE439 pKa = 3.36IAGLIKK445 pKa = 10.31EE446 pKa = 3.95ISEE449 pKa = 3.9NRR451 pKa = 11.84NAPTASKK458 pKa = 10.11PVGAAGPRR466 pKa = 11.84KK467 pKa = 9.75QPVSAQKK474 pKa = 10.35QAVARR479 pKa = 11.84PKK481 pKa = 9.85GTTSSSPKK489 pKa = 10.06VSVTDD494 pKa = 4.28GPEE497 pKa = 3.71LTPVQRR503 pKa = 11.84SQVLQDD509 pKa = 3.12IAKK512 pKa = 9.98SLRR515 pKa = 11.84TLRR518 pKa = 11.84AEE520 pKa = 4.39QPRR523 pKa = 11.84LQQAISKK530 pKa = 9.55KK531 pKa = 9.55SSKK534 pKa = 10.48SPQAASQIGRR544 pKa = 11.84LISLNTEE551 pKa = 3.86LQRR554 pKa = 11.84IISEE558 pKa = 4.4FGLMTPLPTSTEE570 pKa = 3.52TRR572 pKa = 11.84ARR574 pKa = 11.84DD575 pKa = 3.51VASTSVSPPP584 pKa = 3.05

Molecular weight: 64.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEY9|A0A1L3KEY9_9VIRU Capsid protein OS=Wuhan arthropod virus 4 OX=1923693 PE=3 SV=1
MM1 pKa = 7.48VDD3 pKa = 3.03RR4 pKa = 11.84VLFGWLAQAALSNVGKK20 pKa = 8.23TPCAVGWAPVKK31 pKa = 10.73SGWRR35 pKa = 11.84VVRR38 pKa = 11.84HH39 pKa = 6.08RR40 pKa = 11.84FPKK43 pKa = 10.25SICLDD48 pKa = 3.14KK49 pKa = 11.27SAWDD53 pKa = 3.38WTVQPWLITAYY64 pKa = 10.19HH65 pKa = 6.28RR66 pKa = 11.84FVNEE70 pKa = 4.05LALEE74 pKa = 4.37APDD77 pKa = 3.08WWRR80 pKa = 11.84KK81 pKa = 8.47AVHH84 pKa = 6.35SRR86 pKa = 11.84FTLLFRR92 pKa = 11.84AAKK95 pKa = 9.22FQFRR99 pKa = 11.84NGTVIAQPEE108 pKa = 4.37VGVMKK113 pKa = 10.38SGCYY117 pKa = 8.49LTLLLNSVGQVLAHH131 pKa = 6.76IAAKK135 pKa = 10.3LRR137 pKa = 11.84LGEE140 pKa = 4.7DD141 pKa = 3.98PYY143 pKa = 11.59EE144 pKa = 4.31SLPFAMGDD152 pKa = 3.47DD153 pKa = 4.26TVQSPRR159 pKa = 11.84GIHH162 pKa = 6.6DD163 pKa = 3.65LEE165 pKa = 4.94SYY167 pKa = 10.54VRR169 pKa = 11.84EE170 pKa = 4.18LEE172 pKa = 4.25KK173 pKa = 10.95LGCKK177 pKa = 9.95VKK179 pKa = 10.65GAVVRR184 pKa = 11.84PDD186 pKa = 3.09VEE188 pKa = 4.06FAGFQMTDD196 pKa = 3.15RR197 pKa = 11.84RR198 pKa = 11.84CVPAYY203 pKa = 10.0RR204 pKa = 11.84KK205 pKa = 9.24KK206 pKa = 9.97HH207 pKa = 5.33LYY209 pKa = 8.98KK210 pKa = 10.63LEE212 pKa = 4.11YY213 pKa = 9.81ATDD216 pKa = 3.79LPAYY220 pKa = 9.05LRR222 pKa = 11.84SMQLLYY228 pKa = 11.13GHH230 pKa = 7.17DD231 pKa = 3.14HH232 pKa = 5.93TMFSFFHH239 pKa = 5.86NVAARR244 pKa = 11.84KK245 pKa = 9.53CPEE248 pKa = 3.78AAISTSTAMMFMGG261 pKa = 4.98

Molecular weight: 29.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1332

261

584

444.0

49.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.859 ± 0.386

0.976 ± 0.328

4.73 ± 0.181

5.631 ± 0.72

3.529 ± 0.486

5.931 ± 0.56

2.628 ± 0.659

4.129 ± 0.48

5.706 ± 0.272

9.61 ± 0.375

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.315

2.778 ± 0.314

6.757 ± 0.574

4.129 ± 0.61

5.48 ± 0.371

6.832 ± 0.486

6.381 ± 0.956

7.508 ± 0.333

2.402 ± 0.357

3.754 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski