Candidatus Gastranaerophilus sp. (ex Termes propinquus)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1073 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2X4V3|A0A2L2X4V3_9BACT Ribosomal-protein-alanine N-acetyltransferase OS=Candidatus Gastranaerophilus sp. (ex Termes propinquus) OX=2029980 GN=rimI PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.9 KK3 pKa = 10.45 YY4 pKa = 9.53 VCQVCGYY11 pKa = 10.57 VYY13 pKa = 10.47 DD14 pKa = 4.2 PQEE17 pKa = 4.27 GDD19 pKa = 3.58 SVGGVAPGTPFDD31 pKa = 4.82 AVPADD36 pKa = 3.72 WTCPVCGVGKK46 pKa = 10.01 DD47 pKa = 3.6 QFAKK51 pKa = 10.25 QAA53 pKa = 3.43
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.961
IPC2_protein 4.088
IPC_protein 3.91
Toseland 3.706
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.037
Patrickios 0.121
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.941
Protein with the highest isoelectric point:
>tr|A0A2L2X6P3|A0A2L2X6P3_9BACT Putative secondary thiamine-phosphate synthase enzyme OS=Candidatus Gastranaerophilus sp. (ex Termes propinquus) OX=2029980 GN=tpqmel_1011 PE=3 SV=1
EEE2 pKa = 4.3 YY3 pKa = 10.95 LGIAIRR9 pKa = 11.84 NAVVATRR16 pKa = 11.84 AIGFVKKK23 pKa = 10.59 LVAARR28 pKa = 11.84 LLIVL
Molecular weight: 3.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.999
IPC_protein 11.038
Toseland 10.994
ProMoST 11.067
Dawson 11.067
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.067
Grimsley 11.125
Solomon 11.345
Lehninger 11.286
Nozaki 10.965
DTASelect 10.906
Thurlkill 10.994
EMBOSS 11.433
Sillero 11.023
Patrickios 11.023
IPC_peptide 11.345
IPC2_peptide 10.101
IPC2.peptide.svr19 8.341
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1073
0
1073
278237
19
1365
259.3
28.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.003 ± 0.077
1.331 ± 0.032
5.398 ± 0.051
7.421 ± 0.084
4.405 ± 0.052
6.481 ± 0.082
1.652 ± 0.036
7.269 ± 0.068
8.228 ± 0.071
9.455 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.352 ± 0.038
4.961 ± 0.067
3.436 ± 0.049
3.27 ± 0.046
3.974 ± 0.056
6.37 ± 0.069
5.283 ± 0.067
6.737 ± 0.065
0.64 ± 0.02
3.336 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here