Papillomaviridae sp.
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345N151|A0A345N151_9PAPI Protein E8^E2C OS=Papillomaviridae sp. OX=2052558 PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 6.46 FIDD5 pKa = 4.97 TMAEE9 pKa = 4.06 CSDD12 pKa = 3.66 SGGEE16 pKa = 4.06 EE17 pKa = 4.01 EE18 pKa = 5.22 QEE20 pKa = 4.17 VLTVEE25 pKa = 4.48 DD26 pKa = 4.72 EE27 pKa = 4.46 SFIDD31 pKa = 3.29 NTQYY35 pKa = 11.1 CEE37 pKa = 4.05 SPPRR41 pKa = 11.84 KK42 pKa = 9.32 LLKK45 pKa = 10.0 QLCEE49 pKa = 4.14 SLEE52 pKa = 4.04 KK53 pKa = 10.85 SSLEE57 pKa = 3.96 KK58 pKa = 10.56 KK59 pKa = 8.28 YY60 pKa = 11.26 AKK62 pKa = 10.0 YY63 pKa = 9.95 DD64 pKa = 3.47 QPGPSVSKK72 pKa = 10.31 RR73 pKa = 11.84 VLFPEE78 pKa = 4.02 RR79 pKa = 11.84 HH80 pKa = 5.71 VEE82 pKa = 4.1 TQEE85 pKa = 3.82 DD86 pKa = 4.2 SGMLTYY92 pKa = 10.9 DD93 pKa = 3.98 EE94 pKa = 5.72 LSMRR98 pKa = 11.84 DD99 pKa = 3.15 NYY101 pKa = 10.59 EE102 pKa = 3.41 INEE105 pKa = 4.17 LVNTEE110 pKa = 4.19 TLFEE114 pKa = 4.39 EE115 pKa = 4.87 EE116 pKa = 4.62 FIAPVVEE123 pKa = 4.38 VQGTLSAQTLEE134 pKa = 4.16 QKK136 pKa = 10.65 LRR138 pKa = 11.84 TSYY141 pKa = 11.13 YY142 pKa = 9.36 HH143 pKa = 5.75 FTAASSPDD151 pKa = 3.58 KK152 pKa = 11.03 IGVRR156 pKa = 11.84 WNDD159 pKa = 2.86 VHH161 pKa = 8.97 KK162 pKa = 10.87 GMKK165 pKa = 9.64 SPKK168 pKa = 8.57 TMQKK172 pKa = 7.97 IWSIFVEE179 pKa = 4.42 RR180 pKa = 11.84 PVEE183 pKa = 3.98 VRR185 pKa = 11.84 RR186 pKa = 11.84 NHH188 pKa = 6.01 PNCGKK193 pKa = 10.13 RR194 pKa = 11.84 SHH196 pKa = 6.65 VPLEE200 pKa = 4.01 ALLCNDD206 pKa = 3.66 AALLRR211 pKa = 11.84 ASSTPHH217 pKa = 5.44 HH218 pKa = 6.82 VYY220 pKa = 10.89 YY221 pKa = 10.83 LLEE224 pKa = 4.32 YY225 pKa = 9.83 TDD227 pKa = 3.71 SQRR230 pKa = 11.84 SIKK233 pKa = 10.35 GLQKK237 pKa = 10.47 LLKK240 pKa = 10.26 AVGIEE245 pKa = 3.87 HH246 pKa = 7.15 CLFGVPYY253 pKa = 10.09 FKK255 pKa = 10.77 QPLTRR260 pKa = 11.84 TFLRR264 pKa = 11.84 DD265 pKa = 2.96 STTPLDD271 pKa = 3.78 VKK273 pKa = 10.77 RR274 pKa = 11.84 DD275 pKa = 3.61 VSFLADD281 pKa = 3.76 IEE283 pKa = 4.23 DD284 pKa = 3.46 EE285 pKa = 4.51 KK286 pKa = 11.39 YY287 pKa = 10.5 IKK289 pKa = 10.11 QSYY292 pKa = 7.75 QFNMEE297 pKa = 4.11 EE298 pKa = 4.21 LISYY302 pKa = 9.84 CEE304 pKa = 3.94 SSEE307 pKa = 4.24 PEE309 pKa = 4.08 TVQQLIYY316 pKa = 10.39 RR317 pKa = 11.84 YY318 pKa = 7.93 TSEE321 pKa = 4.97 AKK323 pKa = 10.48 HH324 pKa = 6.12 GDD326 pKa = 3.41 PNAIAWRR333 pKa = 11.84 NTTSCMNHH341 pKa = 6.13 AKK343 pKa = 10.29 NAFSMWKK350 pKa = 8.26 STVQGEE356 pKa = 4.27 MLDD359 pKa = 4.4 LSLSDD364 pKa = 4.6 YY365 pKa = 10.18 ILHH368 pKa = 7.07 RR369 pKa = 11.84 ISEE372 pKa = 4.37 NEE374 pKa = 3.81 GGDD377 pKa = 3.38 AAKK380 pKa = 10.3 VRR382 pKa = 11.84 RR383 pKa = 11.84 LFTIQGILEE392 pKa = 3.99 IDD394 pKa = 3.96 FLNTLRR400 pKa = 11.84 KK401 pKa = 8.98 WLVGQHH407 pKa = 6.03 KK408 pKa = 10.27 YY409 pKa = 10.76 NVIVLVGPGNTGKK422 pKa = 10.64 SLFTEE427 pKa = 4.51 ALMKK431 pKa = 10.74 CLGGAFLSWHH441 pKa = 7.25 KK442 pKa = 11.01 DD443 pKa = 3.12 NQYY446 pKa = 9.46 WKK448 pKa = 10.85 SPILGSRR455 pKa = 11.84 FCCIDD460 pKa = 4.94 DD461 pKa = 3.45 ITLAGWKK468 pKa = 10.12 NLDD471 pKa = 3.33 EE472 pKa = 4.62 CEE474 pKa = 4.26 RR475 pKa = 11.84 RR476 pKa = 11.84 ALDD479 pKa = 3.79 GGIVTINKK487 pKa = 9.43 KK488 pKa = 8.32 FTQPTEE494 pKa = 4.18 VKK496 pKa = 10.39 FPPCLITTNTILTDD510 pKa = 3.46 ADD512 pKa = 4.29 FSFLKK517 pKa = 10.6 NRR519 pKa = 11.84 LVWYY523 pKa = 8.33 TFDD526 pKa = 3.44 KK527 pKa = 11.3 VLVTRR532 pKa = 11.84 DD533 pKa = 3.52 GLAEE537 pKa = 4.13 AAVSTADD544 pKa = 3.37 CAAWLLLNRR553 pKa = 11.84 NTLDD557 pKa = 3.33 LL558 pKa = 4.63
Molecular weight: 63.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.274
IPC2_protein 5.334
IPC_protein 5.308
Toseland 5.397
ProMoST 5.512
Dawson 5.385
Bjellqvist 5.474
Wikipedia 5.283
Rodwell 5.321
Grimsley 5.385
Solomon 5.385
Lehninger 5.347
Nozaki 5.55
DTASelect 5.703
Thurlkill 5.486
EMBOSS 5.423
Sillero 5.639
Patrickios 3.999
IPC_peptide 5.397
IPC2_peptide 5.639
IPC2.peptide.svr19 5.618
Protein with the highest isoelectric point:
>tr|A0A345N148|A0A345N148_9PAPI L2 protein OS=Papillomaviridae sp. OX=2052558 PE=4 SV=1
MM1 pKa = 7.4 VNGKK5 pKa = 7.98 QEE7 pKa = 3.81 QMDD10 pKa = 3.55 IGVLLICLMDD20 pKa = 4.4 LFQHH24 pKa = 6.02 SLRR27 pKa = 11.84 LKK29 pKa = 10.35 QVIQMQPLISITDD42 pKa = 3.63 SLILSNRR49 pKa = 11.84 GLVLHH54 pKa = 6.55 HH55 pKa = 6.31 HH56 pKa = 5.46 QKK58 pKa = 10.53 SRR60 pKa = 11.84 LEE62 pKa = 4.12 VIIIFHH68 pKa = 6.04 LTLYY72 pKa = 10.4 IILIRR77 pKa = 11.84 NMYY80 pKa = 8.11 ITRR83 pKa = 11.84 KK84 pKa = 8.79 YY85 pKa = 9.81 RR86 pKa = 11.84 RR87 pKa = 11.84 NVKK90 pKa = 9.11 NAKK93 pKa = 9.21 ARR95 pKa = 11.84 NVLSMMCNYY104 pKa = 9.98 SVSRR108 pKa = 11.84 TYY110 pKa = 10.72 SSGVRR115 pKa = 11.84 VHH117 pKa = 7.03 RR118 pKa = 11.84 GWCTYY123 pKa = 10.46
Molecular weight: 14.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.823
IPC_protein 10.482
Toseland 10.365
ProMoST 10.145
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.818
Grimsley 10.643
Solomon 10.613
Lehninger 10.57
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.482
Patrickios 10.496
IPC_peptide 10.613
IPC2_peptide 9.414
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1936
123
558
387.2
43.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.888 ± 0.574
1.498 ± 0.418
6.043 ± 0.689
6.508 ± 0.602
3.874 ± 0.233
6.198 ± 0.53
3.099 ± 0.458
5.63 ± 0.506
7.128 ± 1.11
8.523 ± 1.133
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.963 ± 0.502
4.029 ± 0.353
6.612 ± 1.7
3.306 ± 0.497
5.837 ± 0.74
6.56 ± 0.575
6.508 ± 0.246
6.043 ± 0.294
1.343 ± 0.177
3.409 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here