Clostridium clostridioforme CAG:132

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6JVG6|R6JVG6_9CLOT Aldedh domain-containing protein OS=Clostridium clostridioforme CAG:132 OX=1263065 GN=BN486_01988 PE=4 SV=1
MM1 pKa = 7.6GRR3 pKa = 11.84LKK5 pKa = 10.13QQAVIWMSGALLMFMCGTAMASTRR29 pKa = 11.84TEE31 pKa = 3.68IDD33 pKa = 3.7SISLDD38 pKa = 3.35VEE40 pKa = 4.87SNIEE44 pKa = 4.19AGDD47 pKa = 3.6SSGDD51 pKa = 3.36VDD53 pKa = 4.13VTCDD57 pKa = 3.05SGDD60 pKa = 3.75YY61 pKa = 10.56YY62 pKa = 11.58VDD64 pKa = 5.08DD65 pKa = 4.34IEE67 pKa = 4.95ITNEE71 pKa = 4.15PKK73 pKa = 10.85NGWDD77 pKa = 4.91DD78 pKa = 3.54GDD80 pKa = 3.86KK81 pKa = 10.88PKK83 pKa = 11.22LKK85 pKa = 9.58VTVEE89 pKa = 4.17AEE91 pKa = 3.48DD92 pKa = 4.61DD93 pKa = 4.5YY94 pKa = 11.69YY95 pKa = 11.36FSSGLSKK102 pKa = 11.17NDD104 pKa = 2.94VDD106 pKa = 5.93LKK108 pKa = 10.98GADD111 pKa = 3.45GKK113 pKa = 9.33VTSVTRR119 pKa = 11.84KK120 pKa = 8.79SSTLIVYY127 pKa = 7.97ITLDD131 pKa = 3.62SLDD134 pKa = 4.56GSDD137 pKa = 4.35SGYY140 pKa = 11.24DD141 pKa = 3.29LDD143 pKa = 5.13VYY145 pKa = 10.83GLEE148 pKa = 4.15WDD150 pKa = 4.45EE151 pKa = 4.43SDD153 pKa = 6.1GMASWEE159 pKa = 4.14DD160 pKa = 3.18SGDD163 pKa = 3.27ARR165 pKa = 11.84KK166 pKa = 10.36YY167 pKa = 8.99EE168 pKa = 3.83VRR170 pKa = 11.84LYY172 pKa = 11.06RR173 pKa = 11.84NDD175 pKa = 3.48NSVTSVLTTSDD186 pKa = 2.78TSYY189 pKa = 11.36DD190 pKa = 3.33FSGYY194 pKa = 7.04ITKK197 pKa = 10.72SGDD200 pKa = 3.12YY201 pKa = 8.26MFKK204 pKa = 10.52VRR206 pKa = 11.84AVYY209 pKa = 10.42NSSDD213 pKa = 3.32KK214 pKa = 11.35GSWEE218 pKa = 4.49EE219 pKa = 3.69SDD221 pKa = 2.96SWYY224 pKa = 10.81VSSEE228 pKa = 3.99EE229 pKa = 4.19ADD231 pKa = 3.94EE232 pKa = 4.74ISDD235 pKa = 3.47GRR237 pKa = 11.84RR238 pKa = 11.84TYY240 pKa = 11.09GSSSSDD246 pKa = 3.32YY247 pKa = 10.61KK248 pKa = 11.17GAWLRR253 pKa = 11.84DD254 pKa = 3.56AVGWWYY260 pKa = 11.29CNADD264 pKa = 3.07KK265 pKa = 10.97SYY267 pKa = 9.96TVNNWQYY274 pKa = 11.27IDD276 pKa = 3.81DD277 pKa = 3.46RR278 pKa = 11.84WYY280 pKa = 9.85FFNAAGYY287 pKa = 8.04MVTGWIDD294 pKa = 3.02WNGRR298 pKa = 11.84WYY300 pKa = 11.2YY301 pKa = 11.01CGDD304 pKa = 4.28DD305 pKa = 3.07GAMYY309 pKa = 10.93YY310 pKa = 8.88DD311 pKa = 3.66TTTPDD316 pKa = 3.07GYY318 pKa = 11.53YY319 pKa = 10.71VGDD322 pKa = 4.24DD323 pKa = 4.06GAWVQQ328 pKa = 4.15

Molecular weight: 36.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6KFZ9|R6KFZ9_9CLOT SSD domain-containing protein OS=Clostridium clostridioforme CAG:132 OX=1263065 GN=BN486_01440 PE=4 SV=1
MM1 pKa = 7.13GVSRR5 pKa = 11.84KK6 pKa = 9.9SISVQSQMCGDD17 pKa = 3.74TQIVVLVIRR26 pKa = 11.84EE27 pKa = 3.95QNRR30 pKa = 11.84DD31 pKa = 3.07LKK33 pKa = 10.89RR34 pKa = 11.84AVARR38 pKa = 11.84TRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84LKK44 pKa = 10.35RR45 pKa = 11.84AALKK49 pKa = 10.3FGKK52 pKa = 9.7CLRR55 pKa = 11.84RR56 pKa = 11.84NWLYY60 pKa = 10.78ILIGLWLTWKK70 pKa = 10.62AIDD73 pKa = 3.35AAYY76 pKa = 10.04EE77 pKa = 3.91FRR79 pKa = 11.84GYY81 pKa = 10.31RR82 pKa = 11.84AIGGEE87 pKa = 4.09YY88 pKa = 10.24LVLPMFLALVVMIRR102 pKa = 11.84KK103 pKa = 6.23TVSFIEE109 pKa = 4.31YY110 pKa = 9.8CRR112 pKa = 11.84EE113 pKa = 3.81GG114 pKa = 3.35

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4152

0

4152

1251376

29

4096

301.4

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.1 ± 0.049

1.588 ± 0.016

5.537 ± 0.03

7.073 ± 0.044

3.989 ± 0.027

8.015 ± 0.041

1.761 ± 0.017

6.864 ± 0.034

5.746 ± 0.029

9.08 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.481 ± 0.019

3.801 ± 0.026

3.657 ± 0.021

3.22 ± 0.023

5.068 ± 0.033

5.833 ± 0.028

5.199 ± 0.028

7.049 ± 0.033

0.991 ± 0.014

3.948 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski