Amino acid dipepetide frequency for Clostridium clostridioforme CAG:132

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.194AlaAla: 9.194 ± 0.118	1.373AlaCys: 1.373 ± 0.036	4.663AlaAsp: 4.663 ± 0.072	5.167AlaGlu: 5.167 ± 0.081	3.135AlaPhe: 3.135 ± 0.053	7.886AlaGly: 7.886 ± 0.103	1.202AlaHis: 1.202 ± 0.033	4.815AlaIle: 4.815 ± 0.073	3.974AlaLys: 3.974 ± 0.064	7.507AlaLeu: 7.507 ± 0.099	2.902AlaMet: 2.902 ± 0.051	2.25AlaAsn: 2.25 ± 0.049	2.384AlaPro: 2.384 ± 0.044	2.393AlaGln: 2.393 ± 0.045	3.93AlaArg: 3.93 ± 0.063	4.402AlaSer: 4.402 ± 0.071	2.906AlaThr: 2.906 ± 0.046	7.331AlaVal: 7.331 ± 0.09	0.744AlaTrp: 0.744 ± 0.023	2.841AlaTyr: 2.841 ± 0.053	0.001AlaXaa: 0.001 ± 0.001
Cys	1.13CysAla: 1.13 ± 0.03	0.35CysCys: 0.35 ± 0.016	0.804CysAsp: 0.804 ± 0.023	0.791CysGlu: 0.791 ± 0.026	0.697CysPhe: 0.697 ± 0.025	1.713CysGly: 1.713 ± 0.039	0.334CysHis: 0.334 ± 0.017	1.102CysIle: 1.102 ± 0.03	0.658CysLys: 0.658 ± 0.025	1.43CysLeu: 1.43 ± 0.041	0.511CysMet: 0.511 ± 0.02	0.527CysAsn: 0.527 ± 0.022	0.758CysPro: 0.758 ± 0.029	0.46CysGln: 0.46 ± 0.019	1.081CysArg: 1.081 ± 0.034	1.013CysSer: 1.013 ± 0.028	0.763CysThr: 0.763 ± 0.025	1.041CysVal: 1.041 ± 0.028	0.12CysTrp: 0.12 ± 0.01	0.595CysTyr: 0.595 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.028AspAla: 4.028 ± 0.067	0.869AspCys: 0.869 ± 0.028	2.588AspAsp: 2.588 ± 0.06	4.064AspGlu: 4.064 ± 0.061	2.46AspPhe: 2.46 ± 0.046	4.921AspGly: 4.921 ± 0.081	0.876AspHis: 0.876 ± 0.031	4.298AspIle: 4.298 ± 0.065	3.113AspLys: 3.113 ± 0.051	4.345AspLeu: 4.345 ± 0.071	2.111AspMet: 2.111 ± 0.042	2.011AspAsn: 2.011 ± 0.041	1.73AspPro: 1.73 ± 0.043	1.548AspGln: 1.548 ± 0.03	3.05AspArg: 3.05 ± 0.056	3.315AspSer: 3.315 ± 0.054	3.018AspThr: 3.018 ± 0.054	3.81AspVal: 3.81 ± 0.05	0.619AspTrp: 0.619 ± 0.02	2.6AspTyr: 2.6 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	6.02GluAla: 6.02 ± 0.086	0.818GluCys: 0.818 ± 0.028	4.338GluAsp: 4.338 ± 0.063	6.398GluGlu: 6.398 ± 0.093	2.324GluPhe: 2.324 ± 0.041	4.848GluGly: 4.848 ± 0.068	1.395GluHis: 1.395 ± 0.031	4.632GluIle: 4.632 ± 0.059	5.038GluLys: 5.038 ± 0.074	6.443GluLeu: 6.443 ± 0.078	2.377GluMet: 2.377 ± 0.041	3.274GluAsn: 3.274 ± 0.054	2.123GluPro: 2.123 ± 0.04	2.918GluGln: 2.918 ± 0.05	3.625GluArg: 3.625 ± 0.058	3.23GluSer: 3.23 ± 0.063	3.567GluThr: 3.567 ± 0.062	3.946GluVal: 3.946 ± 0.058	0.605GluTrp: 0.605 ± 0.025	2.811GluTyr: 2.811 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	2.978PheAla: 2.978 ± 0.054	0.734PheCys: 0.734 ± 0.023	2.338PheAsp: 2.338 ± 0.04	2.281PheGlu: 2.281 ± 0.046	1.707PhePhe: 1.707 ± 0.043	3.284PheGly: 3.284 ± 0.052	0.79PheHis: 0.79 ± 0.025	2.759PheIle: 2.759 ± 0.056	1.995PheLys: 1.995 ± 0.036	3.929PheLeu: 3.929 ± 0.067	1.339PheMet: 1.339 ± 0.038	1.422PheAsn: 1.422 ± 0.035	1.49PhePro: 1.49 ± 0.039	1.163PheGln: 1.163 ± 0.032	1.853PheArg: 1.853 ± 0.043	2.681PheSer: 2.681 ± 0.058	2.292PheThr: 2.292 ± 0.043	2.732PheVal: 2.732 ± 0.047	0.452PheTrp: 0.452 ± 0.024	1.665PheTyr: 1.665 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	6.112GlyAla: 6.112 ± 0.081	1.55GlyCys: 1.55 ± 0.033	3.823GlyAsp: 3.823 ± 0.063	4.968GlyGlu: 4.968 ± 0.062	3.387GlyPhe: 3.387 ± 0.062	5.996GlyGly: 5.996 ± 0.101	1.417GlyHis: 1.417 ± 0.032	6.62GlyIle: 6.62 ± 0.082	4.893GlyLys: 4.893 ± 0.064	6.998GlyLeu: 6.998 ± 0.083	2.953GlyMet: 2.953 ± 0.049	3.139GlyAsn: 3.139 ± 0.056	1.966GlyPro: 1.966 ± 0.037	2.632GlyGln: 2.632 ± 0.046	4.311GlyArg: 4.311 ± 0.065	4.752GlySer: 4.752 ± 0.077	4.773GlyThr: 4.773 ± 0.064	5.514GlyVal: 5.514 ± 0.073	0.921GlyTrp: 0.921 ± 0.032	3.423GlyTyr: 3.423 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	1.107HisAla: 1.107 ± 0.03	0.307HisCys: 0.307 ± 0.015	0.893HisAsp: 0.893 ± 0.026	1.013HisGlu: 1.013 ± 0.026	0.834HisPhe: 0.834 ± 0.024	1.495HisGly: 1.495 ± 0.034	0.406HisHis: 0.406 ± 0.021	1.347HisIle: 1.347 ± 0.033	0.909HisLys: 0.909 ± 0.027	1.568HisLeu: 1.568 ± 0.037	0.678HisMet: 0.678 ± 0.023	0.65HisAsn: 0.65 ± 0.026	0.913HisPro: 0.913 ± 0.027	0.551HisGln: 0.551 ± 0.02	0.865HisArg: 0.865 ± 0.028	0.985HisSer: 0.985 ± 0.028	0.919HisThr: 0.919 ± 0.03	1.229HisVal: 1.229 ± 0.037	0.172HisTrp: 0.172 ± 0.012	0.773HisTyr: 0.773 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.368IleAla: 5.368 ± 0.077	1.325IleCys: 1.325 ± 0.034	3.491IleAsp: 3.491 ± 0.062	3.941IleGlu: 3.941 ± 0.066	2.62IlePhe: 2.62 ± 0.048	5.134IleGly: 5.134 ± 0.068	1.425IleHis: 1.425 ± 0.036	4.661IleIle: 4.661 ± 0.076	3.451IleLys: 3.451 ± 0.053	6.903IleLeu: 6.903 ± 0.082	2.113IleMet: 2.113 ± 0.041	2.613IleAsn: 2.613 ± 0.046	3.367IlePro: 3.367 ± 0.054	2.273IleGln: 2.273 ± 0.05	3.862IleArg: 3.862 ± 0.064	4.56IleSer: 4.56 ± 0.072	3.937IleThr: 3.937 ± 0.057	4.381IleVal: 4.381 ± 0.073	0.686IleTrp: 0.686 ± 0.024	2.53IleTyr: 2.53 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.83LysAla: 4.83 ± 0.07	0.611LysCys: 0.611 ± 0.02	3.578LysAsp: 3.578 ± 0.053	5.344LysGlu: 5.344 ± 0.08	1.462LysPhe: 1.462 ± 0.04	4.324LysGly: 4.324 ± 0.063	0.901LysHis: 0.901 ± 0.026	3.538LysIle: 3.538 ± 0.064	4.466LysLys: 4.466 ± 0.08	4.604LysLeu: 4.604 ± 0.057	1.972LysMet: 1.972 ± 0.04	2.551LysAsn: 2.551 ± 0.051	1.913LysPro: 1.913 ± 0.04	1.931LysGln: 1.931 ± 0.044	2.994LysArg: 2.994 ± 0.051	2.872LysSer: 2.872 ± 0.05	3.093LysThr: 3.093 ± 0.05	3.698LysVal: 3.698 ± 0.06	0.572LysTrp: 0.572 ± 0.022	2.206LysTyr: 2.206 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.604LeuAla: 7.604 ± 0.084	1.583LeuCys: 1.583 ± 0.035	5.211LeuAsp: 5.211 ± 0.069	6.553LeuGlu: 6.553 ± 0.083	3.834LeuPhe: 3.834 ± 0.067	6.593LeuGly: 6.593 ± 0.087	1.509LeuHis: 1.509 ± 0.037	5.647LeuIle: 5.647 ± 0.088	5.887LeuLys: 5.887 ± 0.073	8.43LeuLeu: 8.43 ± 0.114	3.054LeuMet: 3.054 ± 0.048	3.525LeuAsn: 3.525 ± 0.058	3.759LeuPro: 3.759 ± 0.056	2.383LeuGln: 2.383 ± 0.043	4.04LeuArg: 4.04 ± 0.058	6.076LeuSer: 6.076 ± 0.077	5.105LeuThr: 5.105 ± 0.06	5.888LeuVal: 5.888 ± 0.083	0.811LeuTrp: 0.811 ± 0.025	3.303LeuTyr: 3.303 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	3.191MetAla: 3.191 ± 0.047	0.412MetCys: 0.412 ± 0.019	2.254MetAsp: 2.254 ± 0.037	3.036MetGlu: 3.036 ± 0.046	1.124MetPhe: 1.124 ± 0.029	2.682MetGly: 2.682 ± 0.051	0.456MetHis: 0.456 ± 0.018	2.189MetIle: 2.189 ± 0.041	2.517MetLys: 2.517 ± 0.048	2.907MetLeu: 2.907 ± 0.054	1.12MetMet: 1.12 ± 0.032	1.593MetAsn: 1.593 ± 0.037	1.331MetPro: 1.331 ± 0.034	0.945MetGln: 0.945 ± 0.028	1.505MetArg: 1.505 ± 0.036	1.908MetSer: 1.908 ± 0.038	1.955MetThr: 1.955 ± 0.042	2.5MetVal: 2.5 ± 0.04	0.255MetTrp: 0.255 ± 0.014	0.932MetTyr: 0.932 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.775AsnAla: 2.775 ± 0.05	0.575AsnCys: 0.575 ± 0.022	1.797AsnAsp: 1.797 ± 0.044	2.206AsnGlu: 2.206 ± 0.045	1.371AsnPhe: 1.371 ± 0.03	3.183AsnGly: 3.183 ± 0.058	0.75AsnHis: 0.75 ± 0.027	2.826AsnIle: 2.826 ± 0.051	1.896AsnLys: 1.896 ± 0.042	3.44AsnLeu: 3.44 ± 0.053	1.401AsnMet: 1.401 ± 0.034	1.371AsnAsn: 1.371 ± 0.038	2.039AsnPro: 2.039 ± 0.04	1.498AsnGln: 1.498 ± 0.037	2.124AsnArg: 2.124 ± 0.039	2.136AsnSer: 2.136 ± 0.047	2.047AsnThr: 2.047 ± 0.045	2.585AsnVal: 2.585 ± 0.046	0.417AsnTrp: 0.417 ± 0.023	1.572AsnTyr: 1.572 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	3.083ProAla: 3.083 ± 0.05	0.535ProCys: 0.535 ± 0.023	2.573ProAsp: 2.573 ± 0.05	3.433ProGlu: 3.433 ± 0.054	1.61ProPhe: 1.61 ± 0.043	3.246ProGly: 3.246 ± 0.062	0.688ProHis: 0.688 ± 0.022	2.0ProIle: 2.0 ± 0.041	1.567ProLys: 1.567 ± 0.037	3.035ProLeu: 3.035 ± 0.048	1.161ProMet: 1.161 ± 0.034	1.17ProAsn: 1.17 ± 0.034	0.965ProPro: 0.965 ± 0.027	1.162ProGln: 1.162 ± 0.027	1.351ProArg: 1.351 ± 0.032	2.132ProSer: 2.132 ± 0.039	1.445ProThr: 1.445 ± 0.032	3.501ProVal: 3.501 ± 0.057	0.375ProTrp: 0.375 ± 0.018	1.542ProTyr: 1.542 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.845GlnAla: 2.845 ± 0.054	0.418GlnCys: 0.418 ± 0.019	1.656GlnAsp: 1.656 ± 0.033	2.579GlnGlu: 2.579 ± 0.058	1.18GlnPhe: 1.18 ± 0.031	2.401GlnGly: 2.401 ± 0.044	0.409GlnHis: 0.409 ± 0.019	2.337GlnIle: 2.337 ± 0.043	2.128GlnLys: 2.128 ± 0.045	2.653GlnLeu: 2.653 ± 0.05	1.313GlnMet: 1.313 ± 0.029	1.364GlnAsn: 1.364 ± 0.033	1.03GlnPro: 1.03 ± 0.029	1.039GlnGln: 1.039 ± 0.034	1.351GlnArg: 1.351 ± 0.031	1.722GlnSer: 1.722 ± 0.039	1.588GlnThr: 1.588 ± 0.035	2.414GlnVal: 2.414 ± 0.047	0.404GlnTrp: 0.404 ± 0.018	1.366GlnTyr: 1.366 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.23ArgAla: 3.23 ± 0.056	0.742ArgCys: 0.742 ± 0.03	2.72ArgAsp: 2.72 ± 0.056	4.529ArgGlu: 4.529 ± 0.072	2.063ArgPhe: 2.063 ± 0.038	3.131ArgGly: 3.131 ± 0.045	0.929ArgHis: 0.929 ± 0.026	3.865ArgIle: 3.865 ± 0.059	3.282ArgLys: 3.282 ± 0.051	4.739ArgLeu: 4.739 ± 0.072	1.914ArgMet: 1.914 ± 0.04	2.082ArgAsn: 2.082 ± 0.04	1.767ArgPro: 1.767 ± 0.04	2.041ArgGln: 2.041 ± 0.04	2.959ArgArg: 2.959 ± 0.059	2.445ArgSer: 2.445 ± 0.047	2.58ArgThr: 2.58 ± 0.043	3.069ArgVal: 3.069 ± 0.056	0.478ArgTrp: 0.478 ± 0.021	2.119ArgTyr: 2.119 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.427SerAla: 4.427 ± 0.066	0.913SerCys: 0.913 ± 0.027	2.895SerAsp: 2.895 ± 0.051	3.247SerGlu: 3.247 ± 0.053	2.714SerPhe: 2.714 ± 0.048	5.721SerGly: 5.721 ± 0.087	1.201SerHis: 1.201 ± 0.03	3.97SerIle: 3.97 ± 0.059	2.539SerLys: 2.539 ± 0.04	5.569SerLeu: 5.569 ± 0.074	2.098SerMet: 2.098 ± 0.039	1.938SerAsn: 1.938 ± 0.046	2.115SerPro: 2.115 ± 0.057	2.094SerGln: 2.094 ± 0.044	3.393SerArg: 3.393 ± 0.054	3.562SerSer: 3.562 ± 0.061	2.732SerThr: 2.732 ± 0.049	4.213SerVal: 4.213 ± 0.056	0.621SerTrp: 0.621 ± 0.023	2.369SerTyr: 2.369 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.818ThrAla: 4.818 ± 0.07	0.726ThrCys: 0.726 ± 0.025	2.994ThrAsp: 2.994 ± 0.051	3.313ThrGlu: 3.313 ± 0.055	2.014ThrPhe: 2.014 ± 0.045	5.213ThrGly: 5.213 ± 0.071	0.826ThrHis: 0.826 ± 0.029	3.604ThrIle: 3.604 ± 0.058	2.286ThrLys: 2.286 ± 0.049	4.568ThrLeu: 4.568 ± 0.057	1.609ThrMet: 1.609 ± 0.037	1.706ThrAsn: 1.706 ± 0.039	2.223ThrPro: 2.223 ± 0.043	1.422ThrGln: 1.422 ± 0.033	2.281ThrArg: 2.281 ± 0.042	2.974ThrSer: 2.974 ± 0.046	2.572ThrThr: 2.572 ± 0.055	4.469ThrVal: 4.469 ± 0.06	0.529ThrTrp: 0.529 ± 0.022	1.844ThrTyr: 1.844 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	4.926ValAla: 4.926 ± 0.073	1.264ValCys: 1.264 ± 0.037	3.741ValAsp: 3.741 ± 0.055	4.487ValGlu: 4.487 ± 0.062	3.13ValPhe: 3.13 ± 0.06	4.437ValGly: 4.437 ± 0.066	1.088ValHis: 1.088 ± 0.028	5.221ValIle: 5.221 ± 0.075	4.161ValLys: 4.161 ± 0.06	7.091ValLeu: 7.091 ± 0.087	2.554ValMet: 2.554 ± 0.047	2.747ValAsn: 2.747 ± 0.048	2.92ValPro: 2.92 ± 0.047	1.959ValGln: 1.959 ± 0.04	3.506ValArg: 3.506 ± 0.055	4.758ValSer: 4.758 ± 0.058	4.115ValThr: 4.115 ± 0.062	4.99ValVal: 4.99 ± 0.078	0.66ValTrp: 0.66 ± 0.024	2.739ValTyr: 2.739 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.627TrpAla: 0.627 ± 0.024	0.164TrpCys: 0.164 ± 0.013	0.609TrpAsp: 0.609 ± 0.022	0.639TrpGlu: 0.639 ± 0.022	0.433TrpPhe: 0.433 ± 0.019	0.737TrpGly: 0.737 ± 0.026	0.169TrpHis: 0.169 ± 0.011	0.647TrpIle: 0.647 ± 0.023	0.765TrpLys: 0.765 ± 0.02	0.973TrpLeu: 0.973 ± 0.028	0.369TrpMet: 0.369 ± 0.017	0.579TrpAsn: 0.579 ± 0.024	0.275TrpPro: 0.275 ± 0.016	0.395TrpGln: 0.395 ± 0.02	0.417TrpArg: 0.417 ± 0.018	0.535TrpSer: 0.535 ± 0.022	0.451TrpThr: 0.451 ± 0.02	0.586TrpVal: 0.586 ± 0.022	0.12TrpTrp: 0.12 ± 0.01	0.422TrpTyr: 0.422 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.707TyrAla: 2.707 ± 0.045	0.609TyrCys: 0.609 ± 0.02	2.406TyrAsp: 2.406 ± 0.044	2.738TyrGlu: 2.738 ± 0.04	1.787TyrPhe: 1.787 ± 0.038	3.204TyrGly: 3.204 ± 0.052	0.839TyrHis: 0.839 ± 0.025	2.564TyrIle: 2.564 ± 0.049	1.934TyrLys: 1.934 ± 0.045	3.665TyrLeu: 3.665 ± 0.052	1.264TyrMet: 1.264 ± 0.03	1.502TyrAsn: 1.502 ± 0.038	1.503TyrPro: 1.503 ± 0.037	1.393TyrGln: 1.393 ± 0.04	2.184TyrArg: 2.184 ± 0.052	2.27TyrSer: 2.27 ± 0.045	2.132TyrThr: 2.132 ± 0.045	2.595TyrVal: 2.595 ± 0.048	0.353TyrTrp: 0.353 ± 0.017	1.83TyrTyr: 1.83 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 4152 proteins (1251376 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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