Gordonia malaquae NBRC 108250

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia malaquae

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M3TBX3|M3TBX3_9ACTN Putative adenylate cyclase OS=Gordonia malaquae NBRC 108250 OX=1223542 GN=GM1_005_00890 PE=3 SV=1
MM1 pKa = 7.61NEE3 pKa = 3.89PLQPSVAITAPDD15 pKa = 3.88RR16 pKa = 11.84PRR18 pKa = 11.84AWIGSLAAYY27 pKa = 9.67NGSHH31 pKa = 7.22DD32 pKa = 3.97GVPRR36 pKa = 11.84LIGGWYY42 pKa = 9.67DD43 pKa = 2.99ATDD46 pKa = 3.57AVDD49 pKa = 3.44VTVADD54 pKa = 3.92LHH56 pKa = 7.01AGTGVPFTDD65 pKa = 4.48DD66 pKa = 3.59DD67 pKa = 3.94EE68 pKa = 6.72LYY70 pKa = 11.34VLDD73 pKa = 6.3LDD75 pKa = 5.34GEE77 pKa = 4.69WPVHH81 pKa = 6.85RR82 pKa = 11.84EE83 pKa = 3.65MDD85 pKa = 3.77PAEE88 pKa = 4.0AARR91 pKa = 11.84WGEE94 pKa = 3.83IYY96 pKa = 10.79EE97 pKa = 4.33EE98 pKa = 4.61VGATMWPALCAWVRR112 pKa = 11.84SGSYY116 pKa = 9.48VAEE119 pKa = 4.82GDD121 pKa = 2.75TDD123 pKa = 4.07YY124 pKa = 11.45PVIGDD129 pKa = 3.51FEE131 pKa = 4.06EE132 pKa = 5.87RR133 pKa = 11.84YY134 pKa = 10.33AGEE137 pKa = 3.88WDD139 pKa = 3.8SFDD142 pKa = 6.55DD143 pKa = 4.03YY144 pKa = 11.9AQDD147 pKa = 3.57LAEE150 pKa = 5.84GIGLLDD156 pKa = 3.87GVSDD160 pKa = 3.64EE161 pKa = 4.28VQRR164 pKa = 11.84YY165 pKa = 8.56FDD167 pKa = 3.44WASWTRR173 pKa = 11.84DD174 pKa = 2.98LRR176 pKa = 11.84YY177 pKa = 10.2DD178 pKa = 4.35YY179 pKa = 11.1SVQDD183 pKa = 4.03CPTGGVFVFRR193 pKa = 11.84DD194 pKa = 3.58TT195 pKa = 4.2

Molecular weight: 21.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M3V004|M3V004_9ACTN P22_AR_N domain-containing protein OS=Gordonia malaquae NBRC 108250 OX=1223542 GN=GM1_041_00280 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.92GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4269

0

4269

1358179

47

8234

318.1

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.818 ± 0.046

0.705 ± 0.01

6.943 ± 0.037

5.106 ± 0.035

3.0 ± 0.02

8.848 ± 0.03

2.04 ± 0.017

4.399 ± 0.03

2.291 ± 0.032

9.379 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.017

2.126 ± 0.018

5.382 ± 0.029

2.647 ± 0.022

7.208 ± 0.036

5.954 ± 0.028

6.374 ± 0.027

9.264 ± 0.037

1.441 ± 0.016

2.015 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski