Ctenophore-associated circular genome 1
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJB3|A0A141MJB3_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 1 OX=1778570 PE=3 SV=1
MM1 pKa = 7.84 PKK3 pKa = 10.5 LSDD6 pKa = 3.51 NRR8 pKa = 11.84 VVNYY12 pKa = 9.86 VFTWNNYY19 pKa = 7.47 TDD21 pKa = 3.82 DD22 pKa = 3.67 VKK24 pKa = 11.58 AFIGNFANDD33 pKa = 3.29 YY34 pKa = 9.95 CKK36 pKa = 9.84 WLVYY40 pKa = 10.54 GEE42 pKa = 4.34 EE43 pKa = 4.37 VGASGTPHH51 pKa = 6.46 LQGCFSLYY59 pKa = 9.92 DD60 pKa = 3.67 KK61 pKa = 11.2 KK62 pKa = 10.99 RR63 pKa = 11.84 ITQLCKK69 pKa = 10.58 LGFKK73 pKa = 10.45 AHH75 pKa = 7.0 LEE77 pKa = 3.99 PMKK80 pKa = 10.66 GSPKK84 pKa = 9.96 QAIAYY89 pKa = 7.12 CKK91 pKa = 9.95 KK92 pKa = 10.41 DD93 pKa = 3.27 GSFTEE98 pKa = 4.64 LGDD101 pKa = 3.47 VTRR104 pKa = 11.84 GQGEE108 pKa = 4.23 RR109 pKa = 11.84 NDD111 pKa = 3.73 LKK113 pKa = 10.84 RR114 pKa = 11.84 ACEE117 pKa = 4.11 LIKK120 pKa = 10.36 EE121 pKa = 4.5 GKK123 pKa = 9.75 SISEE127 pKa = 4.25 VADD130 pKa = 3.65 ACPTTYY136 pKa = 10.67 VKK138 pKa = 10.68 YY139 pKa = 10.69 SRR141 pKa = 11.84 GLRR144 pKa = 11.84 EE145 pKa = 3.85 LALVASEE152 pKa = 4.61 AYY154 pKa = 8.84 EE155 pKa = 4.17 HH156 pKa = 6.56 EE157 pKa = 4.61 SVRR160 pKa = 11.84 GVWIYY165 pKa = 10.95 GQTGVGKK172 pKa = 9.75 SHH174 pKa = 7.96 LARR177 pKa = 11.84 DD178 pKa = 4.24 VYY180 pKa = 11.26 PNLFDD185 pKa = 4.77 KK186 pKa = 11.08 SQSKK190 pKa = 7.92 WWDD193 pKa = 4.15 GYY195 pKa = 10.7 CNEE198 pKa = 4.43 HH199 pKa = 6.15 TVLIDD204 pKa = 6.14 DD205 pKa = 5.98 LDD207 pKa = 4.16 TDD209 pKa = 4.6 CLGHH213 pKa = 5.94 YY214 pKa = 9.58 LKK216 pKa = 10.65 RR217 pKa = 11.84 WADD220 pKa = 3.39 KK221 pKa = 10.05 YY222 pKa = 11.24 ACTGEE227 pKa = 4.49 TKK229 pKa = 10.72 GGTIKK234 pKa = 10.46 LQHH237 pKa = 6.25 RR238 pKa = 11.84 VFVVTSNYY246 pKa = 10.07 LPWEE250 pKa = 3.94 LHH252 pKa = 6.33 KK253 pKa = 11.15 DD254 pKa = 3.5 DD255 pKa = 5.79 LMAEE259 pKa = 4.68 AISRR263 pKa = 11.84 RR264 pKa = 11.84 FTIYY268 pKa = 10.36 HH269 pKa = 6.51 KK270 pKa = 7.8 EE271 pKa = 4.01 TRR273 pKa = 11.84 DD274 pKa = 3.47 QKK276 pKa = 11.63 LEE278 pKa = 4.1 DD279 pKa = 4.42 CEE281 pKa = 4.32 VLTYY285 pKa = 10.93 ADD287 pKa = 3.6 RR288 pKa = 11.84 HH289 pKa = 5.75 NIII292 pKa = 4.56
Molecular weight: 33.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.24
IPC2_protein 6.3
IPC_protein 6.402
Toseland 6.517
ProMoST 6.839
Dawson 6.825
Bjellqvist 6.795
Wikipedia 6.825
Rodwell 6.825
Grimsley 6.62
Solomon 6.839
Lehninger 6.839
Nozaki 7.102
DTASelect 7.205
Thurlkill 7.249
EMBOSS 7.234
Sillero 7.234
Patrickios 3.897
IPC_peptide 6.854
IPC2_peptide 6.927
IPC2.peptide.svr19 6.85
Protein with the highest isoelectric point:
>tr|A0A141MJB3|A0A141MJB3_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 1 OX=1778570 PE=3 SV=1
MM1 pKa = 7.84 PKK3 pKa = 10.5 LSDD6 pKa = 3.51 NRR8 pKa = 11.84 VVNYY12 pKa = 9.86 VFTWNNYY19 pKa = 7.47 TDD21 pKa = 3.82 DD22 pKa = 3.67 VKK24 pKa = 11.58 AFIGNFANDD33 pKa = 3.29 YY34 pKa = 9.95 CKK36 pKa = 9.84 WLVYY40 pKa = 10.54 GEE42 pKa = 4.34 EE43 pKa = 4.37 VGASGTPHH51 pKa = 6.46 LQGCFSLYY59 pKa = 9.92 DD60 pKa = 3.67 KK61 pKa = 11.2 KK62 pKa = 10.99 RR63 pKa = 11.84 ITQLCKK69 pKa = 10.58 LGFKK73 pKa = 10.45 AHH75 pKa = 7.0 LEE77 pKa = 3.99 PMKK80 pKa = 10.66 GSPKK84 pKa = 9.96 QAIAYY89 pKa = 7.12 CKK91 pKa = 9.95 KK92 pKa = 10.41 DD93 pKa = 3.27 GSFTEE98 pKa = 4.64 LGDD101 pKa = 3.47 VTRR104 pKa = 11.84 GQGEE108 pKa = 4.23 RR109 pKa = 11.84 NDD111 pKa = 3.73 LKK113 pKa = 10.84 RR114 pKa = 11.84 ACEE117 pKa = 4.11 LIKK120 pKa = 10.36 EE121 pKa = 4.5 GKK123 pKa = 9.75 SISEE127 pKa = 4.25 VADD130 pKa = 3.65 ACPTTYY136 pKa = 10.67 VKK138 pKa = 10.68 YY139 pKa = 10.69 SRR141 pKa = 11.84 GLRR144 pKa = 11.84 EE145 pKa = 3.85 LALVASEE152 pKa = 4.61 AYY154 pKa = 8.84 EE155 pKa = 4.17 HH156 pKa = 6.56 EE157 pKa = 4.61 SVRR160 pKa = 11.84 GVWIYY165 pKa = 10.95 GQTGVGKK172 pKa = 9.75 SHH174 pKa = 7.96 LARR177 pKa = 11.84 DD178 pKa = 4.24 VYY180 pKa = 11.26 PNLFDD185 pKa = 4.77 KK186 pKa = 11.08 SQSKK190 pKa = 7.92 WWDD193 pKa = 4.15 GYY195 pKa = 10.7 CNEE198 pKa = 4.43 HH199 pKa = 6.15 TVLIDD204 pKa = 6.14 DD205 pKa = 5.98 LDD207 pKa = 4.16 TDD209 pKa = 4.6 CLGHH213 pKa = 5.94 YY214 pKa = 9.58 LKK216 pKa = 10.65 RR217 pKa = 11.84 WADD220 pKa = 3.39 KK221 pKa = 10.05 YY222 pKa = 11.24 ACTGEE227 pKa = 4.49 TKK229 pKa = 10.72 GGTIKK234 pKa = 10.46 LQHH237 pKa = 6.25 RR238 pKa = 11.84 VFVVTSNYY246 pKa = 10.07 LPWEE250 pKa = 3.94 LHH252 pKa = 6.33 KK253 pKa = 11.15 DD254 pKa = 3.5 DD255 pKa = 5.79 LMAEE259 pKa = 4.68 AISRR263 pKa = 11.84 RR264 pKa = 11.84 FTIYY268 pKa = 10.36 HH269 pKa = 6.51 KK270 pKa = 7.8 EE271 pKa = 4.01 TRR273 pKa = 11.84 DD274 pKa = 3.47 QKK276 pKa = 11.63 LEE278 pKa = 4.1 DD279 pKa = 4.42 CEE281 pKa = 4.32 VLTYY285 pKa = 10.93 ADD287 pKa = 3.6 RR288 pKa = 11.84 HH289 pKa = 5.75 NIII292 pKa = 4.56
Molecular weight: 33.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.24
IPC2_protein 6.3
IPC_protein 6.402
Toseland 6.517
ProMoST 6.839
Dawson 6.825
Bjellqvist 6.795
Wikipedia 6.825
Rodwell 6.825
Grimsley 6.62
Solomon 6.839
Lehninger 6.839
Nozaki 7.102
DTASelect 7.205
Thurlkill 7.249
EMBOSS 7.234
Sillero 7.234
Patrickios 3.897
IPC_peptide 6.854
IPC2_peptide 6.927
IPC2.peptide.svr19 6.85
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
292
292
292
292.0
33.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.164 ± 0.0
3.425 ± 0.0
7.534 ± 0.0
6.507 ± 0.0
3.082 ± 0.0
7.534 ± 0.0
3.425 ± 0.0
4.11 ± 0.0
8.562 ± 0.0
8.562 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.027 ± 0.0
3.767 ± 0.0
2.397 ± 0.0
2.74 ± 0.0
5.137 ± 0.0
5.137 ± 0.0
6.164 ± 0.0
6.507 ± 0.0
2.397 ± 0.0
5.822 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here