Wenzhou pacific spadenose shark paramyxovirus
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GMX6|A0A2P1GMX6_9MONO Putative glycoprotein OS=Wenzhou pacific spadenose shark paramyxovirus OX=2116452 PE=4 SV=1
MM1 pKa = 7.75 ALTFKK6 pKa = 10.56 GIKK9 pKa = 9.41 SGRR12 pKa = 11.84 SGLGKK17 pKa = 10.11 SGKK20 pKa = 10.32 LSMDD24 pKa = 3.61 EE25 pKa = 4.26 LCEE28 pKa = 4.16 LSCSPFVSSAANPAFGGEE46 pKa = 4.27 GSRR49 pKa = 11.84 VALISDD55 pKa = 4.1 EE56 pKa = 4.46 SVAAAPLFIYY66 pKa = 10.38 PDD68 pKa = 3.97 DD69 pKa = 4.31 KK70 pKa = 11.57 PEE72 pKa = 4.07 EE73 pKa = 4.0 FWVDD77 pKa = 3.0 LFRR80 pKa = 11.84 IFGATFGGGTLNEE93 pKa = 4.41 VARR96 pKa = 11.84 EE97 pKa = 3.92 AALAAVLLSPFKK109 pKa = 11.1 NGIDD113 pKa = 3.6 LAKK116 pKa = 10.26 EE117 pKa = 3.81 VAKK120 pKa = 11.04 NSGASPEE127 pKa = 4.1 IVLLDD132 pKa = 3.86 LSPTGRR138 pKa = 11.84 IITRR142 pKa = 11.84 DD143 pKa = 3.67 PASKK147 pKa = 9.64 HH148 pKa = 5.89 APMLGDD154 pKa = 6.11 LIDD157 pKa = 3.87 QGDD160 pKa = 4.04 ANLQSRR166 pKa = 11.84 LFMAKK171 pKa = 9.34 SWDD174 pKa = 3.7 LDD176 pKa = 3.51 QEE178 pKa = 4.31 IPEE181 pKa = 4.1 EE182 pKa = 4.58 AEE184 pKa = 4.06 LIRR187 pKa = 11.84 AIFCQIYY194 pKa = 9.48 GFSGKK199 pKa = 10.46 AMANYY204 pKa = 9.68 VEE206 pKa = 5.3 AYY208 pKa = 10.47 ASAKK212 pKa = 9.73 LHH214 pKa = 5.98 NKK216 pKa = 8.63 VFYY219 pKa = 9.72 TNRR222 pKa = 11.84 FITNSLVITAHH233 pKa = 6.63 GATQIVDD240 pKa = 4.14 IIRR243 pKa = 11.84 ASGQIRR249 pKa = 11.84 HH250 pKa = 6.21 LLVAEE255 pKa = 4.55 ILASGASTGGGGAIGEE271 pKa = 4.81 IIRR274 pKa = 11.84 HH275 pKa = 5.16 HH276 pKa = 6.35 AVYY279 pKa = 10.32 IAGTQMFQYY288 pKa = 10.37 YY289 pKa = 7.59 VTVQNVMKK297 pKa = 10.36 SRR299 pKa = 11.84 RR300 pKa = 11.84 ALASISGAVGEE311 pKa = 4.42 ARR313 pKa = 11.84 TLIKK317 pKa = 10.61 LMRR320 pKa = 11.84 LQARR324 pKa = 11.84 YY325 pKa = 8.87 GALGPFVKK333 pKa = 10.4 FLCLPDD339 pKa = 4.4 HH340 pKa = 6.23 NQFSGNAYY348 pKa = 9.23 PSLFSMALGIEE359 pKa = 4.19 DD360 pKa = 3.81 VKK362 pKa = 11.43 GKK364 pKa = 8.84 TSHH367 pKa = 6.9 KK368 pKa = 8.37 YY369 pKa = 8.52 VHH371 pKa = 7.3 DD372 pKa = 3.45 ISYY375 pKa = 10.78 VNKK378 pKa = 10.26 SLYY381 pKa = 10.33 EE382 pKa = 3.85 LGKK385 pKa = 10.36 EE386 pKa = 3.56 IGRR389 pKa = 11.84 ASFLEE394 pKa = 4.01 MDD396 pKa = 3.66 QSALNEE402 pKa = 4.16 LEE404 pKa = 4.29 VTAEE408 pKa = 3.76 EE409 pKa = 4.39 AANMDD414 pKa = 3.96 RR415 pKa = 11.84 VNRR418 pKa = 11.84 AALKK422 pKa = 10.85 ALMDD426 pKa = 5.23 DD427 pKa = 5.69 DD428 pKa = 5.2 YY429 pKa = 11.97 DD430 pKa = 5.87 SDD432 pKa = 3.91 DD433 pKa = 4.18 QEE435 pKa = 4.08 II436 pKa = 3.74
Molecular weight: 47.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.316
IPC2_protein 5.334
IPC_protein 5.308
Toseland 5.436
ProMoST 5.525
Dawson 5.397
Bjellqvist 5.486
Wikipedia 5.308
Rodwell 5.334
Grimsley 5.436
Solomon 5.397
Lehninger 5.359
Nozaki 5.563
DTASelect 5.728
Thurlkill 5.512
EMBOSS 5.474
Sillero 5.652
Patrickios 4.151
IPC_peptide 5.41
IPC2_peptide 5.652
IPC2.peptide.svr19 5.622
Protein with the highest isoelectric point:
>tr|A0A2P1GMY3|A0A2P1GMY3_9MONO Hemagglutinin-neuraminidase OS=Wenzhou pacific spadenose shark paramyxovirus OX=2116452 PE=4 SV=1
MM1 pKa = 7.24 TSVSGSKK8 pKa = 9.97 PLWRR12 pKa = 11.84 RR13 pKa = 11.84 FLSSALRR20 pKa = 11.84 SLRR23 pKa = 11.84 TRR25 pKa = 11.84 SFRR28 pKa = 11.84 YY29 pKa = 8.01 RR30 pKa = 11.84 TLSQTSQSSDD40 pKa = 3.14 PRR42 pKa = 11.84 HH43 pKa = 6.46 LPTPPHH49 pKa = 6.89 PSPTSSPEE57 pKa = 3.61 TSTEE61 pKa = 4.07 SIYY64 pKa = 11.23 CVMNPLSAPLPCEE77 pKa = 3.38 TWTEE81 pKa = 3.43 QDD83 pKa = 3.15 TYY85 pKa = 8.65 KK86 pKa = 10.67 TSWIQPQCQGRR97 pKa = 11.84 GRR99 pKa = 11.84 KK100 pKa = 8.6 FSKK103 pKa = 9.21 GTPVTYY109 pKa = 10.86 IMTDD113 pKa = 2.74 KK114 pKa = 10.95 GLEE117 pKa = 3.86 EE118 pKa = 4.18 ARR120 pKa = 11.84 TYY122 pKa = 10.27 TLL124 pKa = 3.85
Molecular weight: 14.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.945
IPC2_protein 9.165
IPC_protein 9.326
Toseland 9.604
ProMoST 9.487
Dawson 9.911
Bjellqvist 9.721
Wikipedia 10.131
Rodwell 10.072
Grimsley 10.014
Solomon 9.984
Lehninger 9.94
Nozaki 9.78
DTASelect 9.663
Thurlkill 9.75
EMBOSS 10.043
Sillero 9.882
Patrickios 5.156
IPC_peptide 9.97
IPC2_peptide 8.799
IPC2.peptide.svr19 7.944
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
6480
124
2164
648.0
72.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.648 ± 0.556
1.59 ± 0.23
5.247 ± 0.448
5.88 ± 0.67
3.565 ± 0.377
5.741 ± 0.295
2.361 ± 0.163
7.33 ± 0.587
5.556 ± 0.989
10.309 ± 0.602
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.167
3.704 ± 0.153
5.664 ± 0.631
4.028 ± 0.273
5.37 ± 0.446
8.812 ± 0.503
6.404 ± 0.394
5.802 ± 0.431
1.019 ± 0.169
3.58 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here