Klebsiella phage ST101-KPC2phi6.3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MGA8|A0A482MGA8_9CAUD Uncharacterized protein OS=Klebsiella phage ST101-KPC2phi6.3 OX=2510475 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 6.79LILLLRR8 pKa = 11.84IFFLAAIVHH17 pKa = 5.78EE18 pKa = 4.9SEE20 pKa = 4.59DD21 pKa = 3.73PLYY24 pKa = 10.73DD25 pKa = 3.44SHH27 pKa = 7.99YY28 pKa = 10.35PEE30 pKa = 4.69YY31 pKa = 10.02KK32 pKa = 9.71TDD34 pKa = 3.63EE35 pKa = 4.39FCC37 pKa = 6.04

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482ME82|A0A482ME82_9CAUD Integrase OS=Klebsiella phage ST101-KPC2phi6.3 OX=2510475 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 9.93NIPVIKK8 pKa = 9.2PLVVVVYY15 pKa = 8.89EE16 pKa = 4.77FIRR19 pKa = 11.84WRR21 pKa = 11.84RR22 pKa = 11.84VRR24 pKa = 11.84YY25 pKa = 7.93WRR27 pKa = 11.84SWWKK31 pKa = 10.72DD32 pKa = 3.06DD33 pKa = 3.84VFFRR37 pKa = 11.84RR38 pKa = 11.84QTAICTRR45 pKa = 11.84DD46 pKa = 3.39YY47 pKa = 11.19FSFEE51 pKa = 3.36SRR53 pKa = 11.84YY54 pKa = 10.2RR55 pKa = 11.84FLKK58 pKa = 10.92LLVACCHH65 pKa = 5.93QRR67 pKa = 11.84GEE69 pKa = 4.19LL70 pKa = 3.6

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12937

37

1128

212.1

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.345 ± 0.474

1.129 ± 0.145

5.535 ± 0.273

6.686 ± 0.219

3.478 ± 0.225

6.957 ± 0.293

1.909 ± 0.211

5.357 ± 0.234

4.916 ± 0.338

9.438 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.134

3.788 ± 0.237

4.653 ± 0.345

4.128 ± 0.314

6.439 ± 0.265

6.655 ± 0.355

5.79 ± 0.356

6.609 ± 0.283

1.654 ± 0.149

2.852 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski