Amino acid dipepetide frequency for Escherichia phage Lambda_ev099

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.341AlaAla: 11.341 ± 1.686
0.654AlaCys: 0.654 ± 0.306
5.016AlaAsp: 5.016 ± 0.548
6.979AlaGlu: 6.979 ± 0.644
3.635AlaPhe: 3.635 ± 0.48
7.27AlaGly: 7.27 ± 0.823
2.035AlaHis: 2.035 ± 0.667
5.67AlaIle: 5.67 ± 0.586
3.489AlaLys: 3.489 ± 0.553
8.215AlaLeu: 8.215 ± 0.911
2.981AlaMet: 2.981 ± 0.486
2.908AlaAsn: 2.908 ± 0.442
2.472AlaPro: 2.472 ± 0.493
4.289AlaGln: 4.289 ± 0.979
6.688AlaArg: 6.688 ± 1.017
7.197AlaSer: 7.197 ± 0.702
5.307AlaThr: 5.307 ± 0.84
6.179AlaVal: 6.179 ± 0.736
1.963AlaTrp: 1.963 ± 0.386
2.981AlaTyr: 2.981 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.8CysAla: 0.8 ± 0.26
0.509CysCys: 0.509 ± 0.222
0.872CysAsp: 0.872 ± 0.277
0.654CysGlu: 0.654 ± 0.238
0.218CysPhe: 0.218 ± 0.13
0.509CysGly: 0.509 ± 0.183
0.291CysHis: 0.291 ± 0.14
1.018CysIle: 1.018 ± 0.279
0.436CysLys: 0.436 ± 0.203
0.8CysLeu: 0.8 ± 0.248
0.218CysMet: 0.218 ± 0.131
0.436CysAsn: 0.436 ± 0.153
0.291CysPro: 0.291 ± 0.134
0.363CysGln: 0.363 ± 0.169
1.236CysArg: 1.236 ± 0.348
1.018CysSer: 1.018 ± 0.286
0.872CysThr: 0.872 ± 0.282
0.582CysVal: 0.582 ± 0.206
0.291CysTrp: 0.291 ± 0.149
0.436CysTyr: 0.436 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
5.888AspAla: 5.888 ± 0.739
0.872AspCys: 0.872 ± 0.239
4.434AspAsp: 4.434 ± 0.54
3.853AspGlu: 3.853 ± 0.624
1.963AspPhe: 1.963 ± 0.443
5.452AspGly: 5.452 ± 0.674
0.509AspHis: 0.509 ± 0.146
4.071AspIle: 4.071 ± 0.697
3.053AspLys: 3.053 ± 0.485
3.926AspLeu: 3.926 ± 0.589
1.527AspMet: 1.527 ± 0.342
2.254AspAsn: 2.254 ± 0.38
2.472AspPro: 2.472 ± 0.556
1.454AspGln: 1.454 ± 0.397
2.617AspArg: 2.617 ± 0.493
2.908AspSer: 2.908 ± 0.412
3.053AspThr: 3.053 ± 0.437
3.635AspVal: 3.635 ± 0.521
1.09AspTrp: 1.09 ± 0.316
1.89AspTyr: 1.89 ± 0.399
0.0AspXaa: 0.0 ± 0.0
Glu
5.961GluAla: 5.961 ± 0.868
0.8GluCys: 0.8 ± 0.288
2.762GluAsp: 2.762 ± 0.381
3.489GluGlu: 3.489 ± 0.597
2.472GluPhe: 2.472 ± 0.507
2.835GluGly: 2.835 ± 0.521
1.163GluHis: 1.163 ± 0.321
3.562GluIle: 3.562 ± 0.432
3.271GluLys: 3.271 ± 0.411
5.816GluLeu: 5.816 ± 0.708
1.599GluMet: 1.599 ± 0.391
2.544GluAsn: 2.544 ± 0.494
1.963GluPro: 1.963 ± 0.386
4.58GluGln: 4.58 ± 0.753
3.635GluArg: 3.635 ± 0.58
3.707GluSer: 3.707 ± 0.575
3.344GluThr: 3.344 ± 0.512
3.271GluVal: 3.271 ± 0.592
1.309GluTrp: 1.309 ± 0.345
2.472GluTyr: 2.472 ± 0.479
0.0GluXaa: 0.0 ± 0.0
Phe
2.181PheAla: 2.181 ± 0.407
0.654PheCys: 0.654 ± 0.209
2.69PheAsp: 2.69 ± 0.507
1.963PheGlu: 1.963 ± 0.387
1.09PhePhe: 1.09 ± 0.291
2.981PheGly: 2.981 ± 0.648
0.872PheHis: 0.872 ± 0.216
1.236PheIle: 1.236 ± 0.328
1.817PheLys: 1.817 ± 0.346
2.617PheLeu: 2.617 ± 0.432
1.018PheMet: 1.018 ± 0.251
1.309PheAsn: 1.309 ± 0.275
1.672PhePro: 1.672 ± 0.289
0.654PheGln: 0.654 ± 0.177
2.835PheArg: 2.835 ± 0.436
3.053PheSer: 3.053 ± 0.632
2.762PheThr: 2.762 ± 0.501
2.617PheVal: 2.617 ± 0.431
0.654PheTrp: 0.654 ± 0.202
1.018PheTyr: 1.018 ± 0.308
0.0PheXaa: 0.0 ± 0.0
Gly
6.179GlyAla: 6.179 ± 0.895
0.436GlyCys: 0.436 ± 0.16
4.871GlyAsp: 4.871 ± 0.44
3.853GlyGlu: 3.853 ± 0.585
2.326GlyPhe: 2.326 ± 0.474
5.016GlyGly: 5.016 ± 0.845
0.582GlyHis: 0.582 ± 0.256
3.562GlyIle: 3.562 ± 0.445
4.362GlyLys: 4.362 ± 0.534
5.379GlyLeu: 5.379 ± 0.697
2.981GlyMet: 2.981 ± 0.535
3.926GlyAsn: 3.926 ± 0.596
1.09GlyPro: 1.09 ± 0.241
3.199GlyGln: 3.199 ± 0.42
3.853GlyArg: 3.853 ± 0.411
4.725GlySer: 4.725 ± 0.615
3.562GlyThr: 3.562 ± 0.764
5.743GlyVal: 5.743 ± 0.655
1.454GlyTrp: 1.454 ± 0.287
2.399GlyTyr: 2.399 ± 0.421
0.0GlyXaa: 0.0 ± 0.0
His
1.018HisAla: 1.018 ± 0.277
0.436HisCys: 0.436 ± 0.17
0.8HisAsp: 0.8 ± 0.24
1.018HisGlu: 1.018 ± 0.286
0.8HisPhe: 0.8 ± 0.263
0.945HisGly: 0.945 ± 0.255
0.218HisHis: 0.218 ± 0.137
0.945HisIle: 0.945 ± 0.232
0.945HisLys: 0.945 ± 0.228
1.817HisLeu: 1.817 ± 0.397
0.436HisMet: 0.436 ± 0.175
0.727HisAsn: 0.727 ± 0.194
0.945HisPro: 0.945 ± 0.289
1.018HisGln: 1.018 ± 0.262
1.163HisArg: 1.163 ± 0.294
1.381HisSer: 1.381 ± 0.395
1.236HisThr: 1.236 ± 0.472
1.09HisVal: 1.09 ± 0.23
0.436HisTrp: 0.436 ± 0.172
0.872HisTyr: 0.872 ± 0.241
0.0HisXaa: 0.0 ± 0.0
Ile
4.871IleAla: 4.871 ± 0.602
1.163IleCys: 1.163 ± 0.308
2.69IleAsp: 2.69 ± 0.495
3.926IleGlu: 3.926 ± 0.621
1.454IlePhe: 1.454 ± 0.346
2.835IleGly: 2.835 ± 0.427
0.872IleHis: 0.872 ± 0.265
2.762IleIle: 2.762 ± 0.49
3.126IleLys: 3.126 ± 0.541
3.489IleLeu: 3.489 ± 0.519
0.8IleMet: 0.8 ± 0.24
2.762IleAsn: 2.762 ± 0.436
2.326IlePro: 2.326 ± 0.4
1.89IleGln: 1.89 ± 0.307
3.489IleArg: 3.489 ± 0.614
3.562IleSer: 3.562 ± 0.43
3.926IleThr: 3.926 ± 0.701
2.762IleVal: 2.762 ± 0.41
0.654IleTrp: 0.654 ± 0.252
1.745IleTyr: 1.745 ± 0.438
0.0IleXaa: 0.0 ± 0.0
Lys
5.307LysAla: 5.307 ± 0.714
0.509LysCys: 0.509 ± 0.204
2.69LysAsp: 2.69 ± 0.41
2.835LysGlu: 2.835 ± 0.5
1.236LysPhe: 1.236 ± 0.302
4.362LysGly: 4.362 ± 0.626
1.163LysHis: 1.163 ± 0.38
2.181LysIle: 2.181 ± 0.535
3.562LysLys: 3.562 ± 0.673
3.853LysLeu: 3.853 ± 0.605
1.454LysMet: 1.454 ± 0.389
2.472LysAsn: 2.472 ± 0.462
2.326LysPro: 2.326 ± 0.372
2.399LysGln: 2.399 ± 0.373
4.144LysArg: 4.144 ± 0.619
3.707LysSer: 3.707 ± 0.456
3.78LysThr: 3.78 ± 0.559
2.908LysVal: 2.908 ± 0.448
1.236LysTrp: 1.236 ± 0.286
1.309LysTyr: 1.309 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
8.796LeuAla: 8.796 ± 1.047
0.8LeuCys: 0.8 ± 0.216
4.144LeuAsp: 4.144 ± 0.62
4.216LeuGlu: 4.216 ± 0.474
2.69LeuPhe: 2.69 ± 0.519
3.707LeuGly: 3.707 ± 0.511
2.035LeuHis: 2.035 ± 0.473
3.417LeuIle: 3.417 ± 0.465
4.58LeuLys: 4.58 ± 0.66
5.743LeuLeu: 5.743 ± 0.745
1.672LeuMet: 1.672 ± 0.338
4.071LeuAsn: 4.071 ± 0.396
4.362LeuPro: 4.362 ± 0.867
3.053LeuGln: 3.053 ± 0.498
5.743LeuArg: 5.743 ± 0.713
6.615LeuSer: 6.615 ± 0.846
6.906LeuThr: 6.906 ± 0.646
3.78LeuVal: 3.78 ± 0.516
1.672LeuTrp: 1.672 ± 0.351
1.672LeuTyr: 1.672 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
3.489MetAla: 3.489 ± 0.674
0.145MetCys: 0.145 ± 0.127
1.236MetAsp: 1.236 ± 0.326
0.727MetGlu: 0.727 ± 0.248
1.09MetPhe: 1.09 ± 0.271
1.163MetGly: 1.163 ± 0.216
0.363MetHis: 0.363 ± 0.195
0.8MetIle: 0.8 ± 0.198
1.963MetLys: 1.963 ± 0.478
2.544MetLeu: 2.544 ± 0.412
0.8MetMet: 0.8 ± 0.216
1.018MetAsn: 1.018 ± 0.326
1.745MetPro: 1.745 ± 0.409
1.381MetGln: 1.381 ± 0.286
2.472MetArg: 2.472 ± 0.399
1.963MetSer: 1.963 ± 0.351
2.69MetThr: 2.69 ± 0.483
1.817MetVal: 1.817 ± 0.4
0.218MetTrp: 0.218 ± 0.117
0.363MetTyr: 0.363 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.216AsnAla: 4.216 ± 0.664
0.582AsnCys: 0.582 ± 0.26
1.963AsnAsp: 1.963 ± 0.363
2.617AsnGlu: 2.617 ± 0.383
1.599AsnPhe: 1.599 ± 0.4
3.998AsnGly: 3.998 ± 0.493
1.018AsnHis: 1.018 ± 0.306
2.472AsnIle: 2.472 ± 0.516
2.69AsnLys: 2.69 ± 0.539
2.472AsnLeu: 2.472 ± 0.335
1.09AsnMet: 1.09 ± 0.347
2.908AsnAsn: 2.908 ± 0.632
2.108AsnPro: 2.108 ± 0.439
1.163AsnGln: 1.163 ± 0.279
2.326AsnArg: 2.326 ± 0.538
2.399AsnSer: 2.399 ± 0.425
2.835AsnThr: 2.835 ± 0.432
2.399AsnVal: 2.399 ± 0.403
0.436AsnTrp: 0.436 ± 0.158
1.599AsnTyr: 1.599 ± 0.339
0.0AsnXaa: 0.0 ± 0.0
Pro
3.707ProAla: 3.707 ± 0.588
0.291ProCys: 0.291 ± 0.134
3.344ProAsp: 3.344 ± 0.576
2.908ProGlu: 2.908 ± 0.507
1.09ProPhe: 1.09 ± 0.303
3.126ProGly: 3.126 ± 0.487
0.872ProHis: 0.872 ± 0.232
1.454ProIle: 1.454 ± 0.355
1.745ProLys: 1.745 ± 0.321
3.344ProLeu: 3.344 ± 0.665
0.872ProMet: 0.872 ± 0.218
1.381ProAsn: 1.381 ± 0.286
2.108ProPro: 2.108 ± 0.45
1.599ProGln: 1.599 ± 0.358
1.745ProArg: 1.745 ± 0.301
3.562ProSer: 3.562 ± 0.627
1.672ProThr: 1.672 ± 0.404
3.78ProVal: 3.78 ± 0.589
0.8ProTrp: 0.8 ± 0.262
0.654ProTyr: 0.654 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
3.926GlnAla: 3.926 ± 0.793
0.509GlnCys: 0.509 ± 0.188
1.236GlnAsp: 1.236 ± 0.24
3.053GlnGlu: 3.053 ± 0.502
1.381GlnPhe: 1.381 ± 0.356
2.035GlnGly: 2.035 ± 0.316
0.509GlnHis: 0.509 ± 0.177
2.908GlnIle: 2.908 ± 0.497
2.69GlnLys: 2.69 ± 0.448
3.562GlnLeu: 3.562 ± 0.504
1.599GlnMet: 1.599 ± 0.328
2.108GlnAsn: 2.108 ± 0.419
1.381GlnPro: 1.381 ± 0.5
2.399GlnGln: 2.399 ± 0.647
2.835GlnArg: 2.835 ± 0.455
3.271GlnSer: 3.271 ± 0.578
2.399GlnThr: 2.399 ± 0.445
3.126GlnVal: 3.126 ± 0.532
0.872GlnTrp: 0.872 ± 0.253
1.381GlnTyr: 1.381 ± 0.307
0.0GlnXaa: 0.0 ± 0.0
Arg
4.289ArgAla: 4.289 ± 0.562
0.509ArgCys: 0.509 ± 0.19
3.489ArgAsp: 3.489 ± 0.692
4.362ArgGlu: 4.362 ± 0.656
2.326ArgPhe: 2.326 ± 0.427
4.434ArgGly: 4.434 ± 0.61
1.309ArgHis: 1.309 ± 0.324
4.144ArgIle: 4.144 ± 0.524
3.562ArgLys: 3.562 ± 0.609
5.67ArgLeu: 5.67 ± 0.615
2.399ArgMet: 2.399 ± 0.422
2.835ArgAsn: 2.835 ± 0.455
1.89ArgPro: 1.89 ± 0.297
3.707ArgGln: 3.707 ± 0.612
6.325ArgArg: 6.325 ± 1.102
3.344ArgSer: 3.344 ± 0.598
3.199ArgThr: 3.199 ± 0.411
3.562ArgVal: 3.562 ± 0.526
1.963ArgTrp: 1.963 ± 0.452
2.544ArgTyr: 2.544 ± 0.42
0.0ArgXaa: 0.0 ± 0.0
Ser
7.56SerAla: 7.56 ± 0.784
0.509SerCys: 0.509 ± 0.209
4.216SerAsp: 4.216 ± 0.654
4.144SerGlu: 4.144 ± 0.496
2.908SerPhe: 2.908 ± 0.445
7.415SerGly: 7.415 ± 0.902
1.236SerHis: 1.236 ± 0.294
2.908SerIle: 2.908 ± 0.436
3.199SerLys: 3.199 ± 0.549
5.016SerLeu: 5.016 ± 0.586
2.181SerMet: 2.181 ± 0.394
1.89SerAsn: 1.89 ± 0.327
2.617SerPro: 2.617 ± 0.503
3.344SerGln: 3.344 ± 0.45
4.58SerArg: 4.58 ± 0.672
3.562SerSer: 3.562 ± 0.665
3.78SerThr: 3.78 ± 0.453
5.67SerVal: 5.67 ± 0.683
1.163SerTrp: 1.163 ± 0.279
1.527SerTyr: 1.527 ± 0.307
0.0SerXaa: 0.0 ± 0.0
Thr
7.051ThrAla: 7.051 ± 0.83
0.945ThrCys: 0.945 ± 0.277
3.998ThrAsp: 3.998 ± 0.425
3.78ThrGlu: 3.78 ± 0.545
2.472ThrPhe: 2.472 ± 0.468
4.434ThrGly: 4.434 ± 0.578
1.599ThrHis: 1.599 ± 0.396
2.181ThrIle: 2.181 ± 0.472
3.126ThrLys: 3.126 ± 0.496
5.743ThrLeu: 5.743 ± 0.657
1.163ThrMet: 1.163 ± 0.368
2.035ThrAsn: 2.035 ± 0.358
3.417ThrPro: 3.417 ± 0.724
2.181ThrGln: 2.181 ± 0.569
2.981ThrArg: 2.981 ± 0.331
3.998ThrSer: 3.998 ± 0.561
4.071ThrThr: 4.071 ± 0.584
3.853ThrVal: 3.853 ± 0.96
1.381ThrTrp: 1.381 ± 0.276
2.326ThrTyr: 2.326 ± 0.521
0.0ThrXaa: 0.0 ± 0.0
Val
6.325ValAla: 6.325 ± 0.927
0.582ValCys: 0.582 ± 0.231
3.78ValAsp: 3.78 ± 0.482
3.635ValGlu: 3.635 ± 0.534
2.835ValPhe: 2.835 ± 0.443
3.344ValGly: 3.344 ± 0.626
0.509ValHis: 0.509 ± 0.176
3.053ValIle: 3.053 ± 0.44
3.78ValLys: 3.78 ± 0.602
5.089ValLeu: 5.089 ± 0.615
1.963ValMet: 1.963 ± 0.393
3.562ValAsn: 3.562 ± 0.534
2.326ValPro: 2.326 ± 0.428
2.326ValGln: 2.326 ± 0.62
3.271ValArg: 3.271 ± 0.461
5.452ValSer: 5.452 ± 0.831
4.725ValThr: 4.725 ± 0.74
4.071ValVal: 4.071 ± 0.601
0.945ValTrp: 0.945 ± 0.269
1.89ValTyr: 1.89 ± 0.486
0.0ValXaa: 0.0 ± 0.0
Trp
1.672TrpAla: 1.672 ± 0.244
0.582TrpCys: 0.582 ± 0.245
1.09TrpAsp: 1.09 ± 0.3
0.509TrpGlu: 0.509 ± 0.162
0.654TrpPhe: 0.654 ± 0.222
1.236TrpGly: 1.236 ± 0.26
0.582TrpHis: 0.582 ± 0.2
1.018TrpIle: 1.018 ± 0.329
1.09TrpLys: 1.09 ± 0.307
2.035TrpLeu: 2.035 ± 0.439
0.727TrpMet: 0.727 ± 0.206
0.727TrpAsn: 0.727 ± 0.249
0.8TrpPro: 0.8 ± 0.243
0.872TrpGln: 0.872 ± 0.247
1.527TrpArg: 1.527 ± 0.425
1.09TrpSer: 1.09 ± 0.234
1.236TrpThr: 1.236 ± 0.246
1.09TrpVal: 1.09 ± 0.309
0.218TrpTrp: 0.218 ± 0.167
0.509TrpTyr: 0.509 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.69TyrAla: 2.69 ± 0.394
0.363TyrCys: 0.363 ± 0.175
1.672TyrAsp: 1.672 ± 0.297
2.035TyrGlu: 2.035 ± 0.472
1.454TyrPhe: 1.454 ± 0.399
2.399TyrGly: 2.399 ± 0.494
0.509TyrHis: 0.509 ± 0.157
1.745TyrIle: 1.745 ± 0.35
0.945TyrLys: 0.945 ± 0.272
2.544TyrLeu: 2.544 ± 0.596
0.436TyrMet: 0.436 ± 0.222
1.018TyrAsn: 1.018 ± 0.195
1.745TyrPro: 1.745 ± 0.385
1.309TyrGln: 1.309 ± 0.304
2.399TyrArg: 2.399 ± 0.425
2.908TyrSer: 2.908 ± 0.581
1.381TyrThr: 1.381 ± 0.345
1.527TyrVal: 1.527 ± 0.336
0.509TyrTrp: 0.509 ± 0.18
1.309TyrTyr: 1.309 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (13757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski