Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Xanthomonas; Xanthomonas campestris; Xanthomonas campestris pv. campestris

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8P4E0|Q8P4E0_XANCP Uncharacterized protein OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC3768 PE=4 SV=1
MM1 pKa = 7.87LDD3 pKa = 3.65LSGGDD8 pKa = 2.97QHH10 pKa = 8.37YY11 pKa = 10.97IEE13 pKa = 5.7DD14 pKa = 4.33CQVCCKK20 pKa = 9.53PISVSVQWDD29 pKa = 3.78EE30 pKa = 4.03EE31 pKa = 4.63GEE33 pKa = 4.28AQVSARR39 pKa = 11.84GQDD42 pKa = 3.49DD43 pKa = 3.34AA44 pKa = 6.75

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8P6Q8|Q8P6Q8_XANCP Sialidase domain-containing protein OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC2909 PE=4 SV=1
MM1 pKa = 7.56IAPTIGGALLYY12 pKa = 9.8RR13 pKa = 11.84LWISRR18 pKa = 11.84PTRR21 pKa = 11.84TAHH24 pKa = 6.37IGLAVGQIPQRR35 pKa = 11.84LRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84AMAVRR45 pKa = 11.84RR46 pKa = 11.84MVAHH50 pKa = 6.94GG51 pKa = 3.42

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4126

1

4127

1418688

21

3961

343.8

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.157 ± 0.058

0.869 ± 0.011

5.714 ± 0.031

4.807 ± 0.031

3.261 ± 0.026

8.307 ± 0.053

2.296 ± 0.024

4.023 ± 0.025

2.658 ± 0.031

10.809 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.019

2.614 ± 0.028

5.36 ± 0.032

4.522 ± 0.029

7.38 ± 0.044

5.481 ± 0.031

5.297 ± 0.04

7.329 ± 0.029

1.591 ± 0.018

2.403 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski