Cereibacter changlensis JA139

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Cereibacter; Cereibacter changlensis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4JSD4|A0A2T4JSD4_9RHOB Elongation factor Tu (Fragment) OS=Cereibacter changlensis JA139 OX=1188249 GN=tuf PE=4 SV=1
MM1 pKa = 6.89THH3 pKa = 6.21SADD6 pKa = 3.9MIEE9 pKa = 4.43GAPLIKK15 pKa = 10.22PSTTDD20 pKa = 2.8HH21 pKa = 7.07PLYY24 pKa = 10.78DD25 pKa = 4.17SVVEE29 pKa = 4.12ACRR32 pKa = 11.84SVFDD36 pKa = 4.4PEE38 pKa = 4.25IPVNIYY44 pKa = 10.88DD45 pKa = 4.22LGLVYY50 pKa = 10.32TIDD53 pKa = 3.22ISPEE57 pKa = 3.91NEE59 pKa = 3.75VEE61 pKa = 4.12ILMTLTAPGCPVAGEE76 pKa = 4.15MPGWVADD83 pKa = 3.75AVEE86 pKa = 5.1PIAGVKK92 pKa = 8.59QVNVGMTFDD101 pKa = 4.79PPWGMEE107 pKa = 3.87MMSDD111 pKa = 3.74EE112 pKa = 4.65ARR114 pKa = 11.84LEE116 pKa = 4.14LGFMM120 pKa = 4.54

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4K038|A0A2T4K038_9RHOB Uncharacterized protein OS=Cereibacter changlensis JA139 OX=1188249 GN=C5F48_01430 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4709

0

4709

1406964

25

4015

298.8

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.458 ± 0.054

0.847 ± 0.011

5.451 ± 0.039

5.864 ± 0.033

3.547 ± 0.023

9.039 ± 0.035

1.923 ± 0.017

4.762 ± 0.03

2.564 ± 0.027

10.94 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.02

2.177 ± 0.022

5.359 ± 0.032

3.084 ± 0.019

7.293 ± 0.047

5.035 ± 0.028

5.298 ± 0.032

7.327 ± 0.031

1.366 ± 0.015

2.004 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski