Flavobacterium phage vB_FspS_morran9-1
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9LDZ4|A0A6B9LDZ4_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_morran9-1 OX=2686258 GN=morran91_gp059 PE=4 SV=1
MM1 pKa = 7.13 TKK3 pKa = 10.25 FVIMKK8 pKa = 7.91 TLKK11 pKa = 10.69 LLLITALAILFLSCSNDD28 pKa = 3.34 DD29 pKa = 4.75 NEE31 pKa = 4.51 QCTYY35 pKa = 9.76 TCNAWVKK42 pKa = 10.29 PDD44 pKa = 3.4 GQIRR48 pKa = 11.84 TVIPVEE54 pKa = 4.25 LNCEE58 pKa = 4.08 TNEE61 pKa = 4.67 PINLPEE67 pKa = 4.47 GYY69 pKa = 9.92 IFLGCDD75 pKa = 3.18 NDD77 pKa = 4.19 NIPP80 pKa = 4.55
Molecular weight: 8.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.021
IPC2_protein 4.329
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 0.782
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.165
Protein with the highest isoelectric point:
>tr|A0A6B9LC93|A0A6B9LC93_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_morran9-1 OX=2686258 GN=morran91_gp032 PE=4 SV=1
MM1 pKa = 7.84 WSRR4 pKa = 11.84 NSEE7 pKa = 4.15 SFSFRR12 pKa = 11.84 LIYY15 pKa = 9.77 RR16 pKa = 11.84 HH17 pKa = 5.53 KK18 pKa = 10.85 NKK20 pKa = 10.48 YY21 pKa = 10.1 KK22 pKa = 10.83 LITNATIAYY31 pKa = 7.4 NRR33 pKa = 11.84 CC34 pKa = 3.15
Molecular weight: 4.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.955
IPC_protein 10.526
Toseland 10.379
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.891
Grimsley 10.657
Solomon 10.628
Lehninger 10.599
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.482
Patrickios 10.716
IPC_peptide 10.628
IPC2_peptide 9.326
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
12113
30
931
165.9
19.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.986 ± 0.393
1.123 ± 0.174
5.531 ± 0.287
7.331 ± 0.349
4.97 ± 0.236
4.689 ± 0.268
1.313 ± 0.202
8.074 ± 0.292
9.527 ± 0.587
8.99 ± 0.281
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.142
8.0 ± 0.315
2.534 ± 0.164
4.053 ± 0.341
3.03 ± 0.173
6.406 ± 0.332
6.497 ± 0.42
5.358 ± 0.199
0.933 ± 0.084
4.392 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here