Dyadobacter fermentans (strain ATCC 700827 / DSM 18053 / CIP 107007 / KCTC 52180 / NS114)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter; Dyadobacter fermentans

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6W1T1|C6W1T1_DYAFD ThuA domain-containing protein OS=Dyadobacter fermentans (strain ATCC 700827 / DSM 18053 / CIP 107007 / KCTC 52180 / NS114) OX=471854 GN=Dfer_4304 PE=4 SV=1
MM1 pKa = 7.04TQDD4 pKa = 2.76NCYY7 pKa = 10.21LLGYY11 pKa = 9.62IVRR14 pKa = 11.84THH16 pKa = 5.52GTAGNVVIYY25 pKa = 10.74LDD27 pKa = 3.19VDD29 pKa = 3.53YY30 pKa = 11.25PEE32 pKa = 6.1DD33 pKa = 3.95YY34 pKa = 11.5GDD36 pKa = 4.35LEE38 pKa = 4.46TVYY41 pKa = 11.07VEE43 pKa = 4.28IKK45 pKa = 10.94GEE47 pKa = 3.97LVPYY51 pKa = 10.15FVDD54 pKa = 4.99EE55 pKa = 4.81INLQKK60 pKa = 10.52QSNAIVTFEE69 pKa = 5.72DD70 pKa = 3.23IDD72 pKa = 4.29TIEE75 pKa = 4.34KK76 pKa = 9.62AQKK79 pKa = 10.37LVGSSLYY86 pKa = 10.1MPLEE90 pKa = 4.39DD91 pKa = 4.57LAEE94 pKa = 4.41LGEE97 pKa = 4.34DD98 pKa = 2.85EE99 pKa = 5.67FYY101 pKa = 10.97YY102 pKa = 10.97HH103 pKa = 7.3EE104 pKa = 4.33IKK106 pKa = 10.62GFKK109 pKa = 10.43VVDD112 pKa = 3.53QTKK115 pKa = 10.13GDD117 pKa = 3.72LGIVRR122 pKa = 11.84EE123 pKa = 4.35VYY125 pKa = 10.6SLNGQDD131 pKa = 5.1LIAMDD136 pKa = 4.07FQGSEE141 pKa = 4.1VLIPTANDD149 pKa = 3.01IVIKK153 pKa = 10.36ADD155 pKa = 3.56KK156 pKa = 9.93DD157 pKa = 3.85AKK159 pKa = 10.64SLLVNLPEE167 pKa = 4.73GLLEE171 pKa = 4.24VYY173 pKa = 10.43LDD175 pKa = 3.9NSSDD179 pKa = 4.57SIPDD183 pKa = 3.68DD184 pKa = 4.13ADD186 pKa = 3.11

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6W286|C6W286_DYAFD Signal transduction histidine kinase LytS OS=Dyadobacter fermentans (strain ATCC 700827 / DSM 18053 / CIP 107007 / KCTC 52180 / NS114) OX=471854 GN=Dfer_0799 PE=4 SV=1
MM1 pKa = 7.19LVSWLRR7 pKa = 11.84RR8 pKa = 11.84SLLLLAEE15 pKa = 4.36NQLRR19 pKa = 11.84MFPVASGPPVSSCKK33 pKa = 9.95PVTHH37 pKa = 6.5VSGPNGASCFWLKK50 pKa = 10.33ISYY53 pKa = 10.44ACLRR57 pKa = 11.84PQRR60 pKa = 11.84GLLFLAKK67 pKa = 10.39AEE69 pKa = 3.93LRR71 pKa = 11.84MLSGSVGASYY81 pKa = 10.42FF82 pKa = 3.45

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5703

0

5703

2075152

31

2418

363.9

40.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.27 ± 0.027

0.734 ± 0.009

5.404 ± 0.019

5.876 ± 0.026

4.786 ± 0.02

7.353 ± 0.033

1.947 ± 0.016

6.329 ± 0.024

5.936 ± 0.028

9.349 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.015

4.917 ± 0.03

4.235 ± 0.02

3.763 ± 0.016

4.888 ± 0.021

6.304 ± 0.024

5.71 ± 0.026

6.718 ± 0.024

1.337 ± 0.013

3.848 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski