Johnsonella ignava ATCC 51276

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Johnsonella; Johnsonella ignava

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5GH96|G5GH96_9FIRM RelA_SpoT domain-containing protein OS=Johnsonella ignava ATCC 51276 OX=679200 GN=HMPREF9333_00936 PE=4 SV=1
MM1 pKa = 7.56SDD3 pKa = 4.13KK4 pKa = 10.75YY5 pKa = 11.55DD6 pKa = 3.7KK7 pKa = 11.54GDD9 pKa = 3.55LFEE12 pKa = 6.99GDD14 pKa = 4.22EE15 pKa = 4.24LTVTIKK21 pKa = 11.16LEE23 pKa = 4.51DD24 pKa = 3.49NTEE27 pKa = 3.87IEE29 pKa = 4.88CIVLNIFEE37 pKa = 4.54VDD39 pKa = 3.01TRR41 pKa = 11.84EE42 pKa = 4.22YY43 pKa = 10.58IAVLPEE49 pKa = 4.18DD50 pKa = 4.4EE51 pKa = 4.54EE52 pKa = 5.58CEE54 pKa = 4.08DD55 pKa = 3.83VYY57 pKa = 11.21LYY59 pKa = 10.58RR60 pKa = 11.84YY61 pKa = 6.03TQKK64 pKa = 11.28DD65 pKa = 3.4DD66 pKa = 4.18GEE68 pKa = 4.59PEE70 pKa = 4.08LTNIEE75 pKa = 3.94SDD77 pKa = 3.57EE78 pKa = 4.24EE79 pKa = 4.41YY80 pKa = 10.7EE81 pKa = 4.18AVVDD85 pKa = 4.04AFEE88 pKa = 5.46EE89 pKa = 4.09ILDD92 pKa = 3.69MQEE95 pKa = 4.15FEE97 pKa = 4.77EE98 pKa = 5.29FLSEE102 pKa = 5.79DD103 pKa = 4.77DD104 pKa = 4.81DD105 pKa = 5.23FKK107 pKa = 11.65DD108 pKa = 3.43

Molecular weight: 12.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5GKK5|G5GKK5_9FIRM Abhydrolase_3 domain-containing protein OS=Johnsonella ignava ATCC 51276 OX=679200 GN=HMPREF9333_02097 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 7.98KK9 pKa = 8.01RR10 pKa = 11.84KK11 pKa = 8.36RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.01GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.83VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84ISAA44 pKa = 3.67

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2284

0

2284

754037

41

3711

330.1

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.713 ± 0.053

1.263 ± 0.02

6.07 ± 0.047

7.178 ± 0.067

4.325 ± 0.038

6.704 ± 0.051

1.541 ± 0.021

8.711 ± 0.056

8.584 ± 0.055

8.572 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.025

5.389 ± 0.043

2.938 ± 0.028

2.528 ± 0.022

4.018 ± 0.04

6.594 ± 0.045

4.792 ± 0.035

6.097 ± 0.04

0.767 ± 0.018

4.45 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski