Firmicutes bacterium CAG:137

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6GXH3|R6GXH3_9FIRM Undecaprenyl-diphosphatase OS=Firmicutes bacterium CAG:137 OX=1263004 GN=uppP PE=3 SV=1
MM1 pKa = 7.27LTNHH5 pKa = 6.22HH6 pKa = 6.88VIDD9 pKa = 4.81GAQSITVTLSDD20 pKa = 3.43GTEE23 pKa = 3.89YY24 pKa = 10.06TAQLVGSDD32 pKa = 3.73SVSDD36 pKa = 3.78VALLKK41 pKa = 10.08IDD43 pKa = 4.26GQDD46 pKa = 3.4LPAVSIGNSDD56 pKa = 3.92DD57 pKa = 5.52LEE59 pKa = 5.13VGDD62 pKa = 4.27QVAAIGNPLGEE73 pKa = 4.4LTSTQTVGYY82 pKa = 9.78VSAKK86 pKa = 9.94DD87 pKa = 3.36RR88 pKa = 11.84SVNTDD93 pKa = 2.78GTIINMLQTDD103 pKa = 3.9AAINSGNSGGPLFNMNGEE121 pKa = 4.47VVGITTAKK129 pKa = 10.21YY130 pKa = 10.3SGSTSSGASIEE141 pKa = 4.64GIGFAIPINDD151 pKa = 3.39VMKK154 pKa = 10.89LVDD157 pKa = 4.89DD158 pKa = 4.11LMEE161 pKa = 4.17YY162 pKa = 10.99GYY164 pKa = 8.53ITGQAYY170 pKa = 10.59LGVTINSKK178 pKa = 10.86DD179 pKa = 3.57LDD181 pKa = 3.95ASTAAAYY188 pKa = 8.14GLPVGARR195 pKa = 11.84VEE197 pKa = 4.54SVTEE201 pKa = 4.2GSCAQKK207 pKa = 10.89AGLQAGDD214 pKa = 4.37IITALGDD221 pKa = 3.67QKK223 pKa = 11.51VSGYY227 pKa = 10.62SDD229 pKa = 3.64LVYY232 pKa = 11.08ALRR235 pKa = 11.84NFSAGDD241 pKa = 3.34ATTVSVYY248 pKa = 10.44RR249 pKa = 11.84AGQEE253 pKa = 3.65LTLQITFDD261 pKa = 3.83EE262 pKa = 4.63KK263 pKa = 10.58TADD266 pKa = 3.6TVTGPVVTTEE276 pKa = 3.99PTEE279 pKa = 3.91ATGDD283 pKa = 3.97GEE285 pKa = 4.83QPSQDD290 pKa = 4.34EE291 pKa = 4.4IPEE294 pKa = 4.34SGSYY298 pKa = 9.66EE299 pKa = 3.61DD300 pKa = 3.75WYY302 pKa = 10.45DD303 pKa = 3.23YY304 pKa = 10.76FYY306 pKa = 11.31RR307 pKa = 11.84FFGGNGNN314 pKa = 3.64

Molecular weight: 32.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6H7T2|R6H7T2_9FIRM CRISPR system Cms protein Csm5 OS=Firmicutes bacterium CAG:137 OX=1263004 GN=BN490_00812 PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.82QPKK8 pKa = 9.37KK9 pKa = 7.72RR10 pKa = 11.84HH11 pKa = 5.14RR12 pKa = 11.84AKK14 pKa = 10.52VHH16 pKa = 5.63GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTTNGRR28 pKa = 11.84KK29 pKa = 8.65VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2347

0

2347

691874

29

2786

294.8

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.386 ± 0.058

1.784 ± 0.024

5.175 ± 0.045

6.442 ± 0.057

3.71 ± 0.034

8.375 ± 0.068

2.139 ± 0.037

5.208 ± 0.045

4.09 ± 0.05

10.365 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.027

3.236 ± 0.038

4.432 ± 0.038

4.205 ± 0.037

5.73 ± 0.064

5.555 ± 0.042

5.592 ± 0.063

7.297 ± 0.052

1.097 ± 0.021

3.541 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski