Amino acid dipepetide frequency for Firmicutes bacterium CAG:137

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.831AlaAla: 10.831 ± 0.16	1.732AlaCys: 1.732 ± 0.048	4.508AlaAsp: 4.508 ± 0.073	5.641AlaGlu: 5.641 ± 0.104	3.376AlaPhe: 3.376 ± 0.094	8.561AlaGly: 8.561 ± 0.138	1.606AlaHis: 1.606 ± 0.052	4.577AlaIle: 4.577 ± 0.087	3.823AlaLys: 3.823 ± 0.094	9.88AlaLeu: 9.88 ± 0.186	2.921AlaMet: 2.921 ± 0.079	2.515AlaAsn: 2.515 ± 0.063	3.826AlaPro: 3.826 ± 0.074	4.119AlaGln: 4.119 ± 0.075	4.612AlaArg: 4.612 ± 0.084	4.775AlaSer: 4.775 ± 0.09	4.264AlaThr: 4.264 ± 0.097	8.107AlaVal: 8.107 ± 0.124	1.107AlaTrp: 1.107 ± 0.038	3.068AlaTyr: 3.068 ± 0.075	0.013AlaXaa: 0.013 ± 0.005
Cys	1.588CysAla: 1.588 ± 0.054	0.334CysCys: 0.334 ± 0.022	0.811CysAsp: 0.811 ± 0.038	0.77CysGlu: 0.77 ± 0.034	0.727CysPhe: 0.727 ± 0.028	1.844CysGly: 1.844 ± 0.054	0.461CysHis: 0.461 ± 0.028	0.922CysIle: 0.922 ± 0.039	0.639CysLys: 0.639 ± 0.031	1.742CysLeu: 1.742 ± 0.048	0.435CysMet: 0.435 ± 0.026	0.595CysAsn: 0.595 ± 0.031	0.922CysPro: 0.922 ± 0.037	0.82CysGln: 0.82 ± 0.039	1.263CysArg: 1.263 ± 0.05	1.036CysSer: 1.036 ± 0.034	1.068CysThr: 1.068 ± 0.048	1.117CysVal: 1.117 ± 0.041	0.16CysTrp: 0.16 ± 0.017	0.582CysTyr: 0.582 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.635AspAla: 4.635 ± 0.086	1.016AspCys: 1.016 ± 0.036	2.235AspAsp: 2.235 ± 0.066	3.112AspGlu: 3.112 ± 0.077	2.362AspPhe: 2.362 ± 0.058	4.898AspGly: 4.898 ± 0.107	1.148AspHis: 1.148 ± 0.04	2.612AspIle: 2.612 ± 0.07	1.734AspLys: 1.734 ± 0.066	5.354AspLeu: 5.354 ± 0.093	1.467AspMet: 1.467 ± 0.045	1.38AspAsn: 1.38 ± 0.045	2.714AspPro: 2.714 ± 0.069	1.828AspGln: 1.828 ± 0.049	3.223AspArg: 3.223 ± 0.075	2.672AspSer: 2.672 ± 0.064	2.856AspThr: 2.856 ± 0.07	3.587AspVal: 3.587 ± 0.073	0.72AspTrp: 0.72 ± 0.035	2.197AspTyr: 2.197 ± 0.061	0.001AspXaa: 0.001 ± 0.002
Glu	6.949GluAla: 6.949 ± 0.106	0.577GluCys: 0.577 ± 0.035	3.295GluAsp: 3.295 ± 0.073	5.403GluGlu: 5.403 ± 0.119	1.846GluPhe: 1.846 ± 0.049	4.927GluGly: 4.927 ± 0.09	1.33GluHis: 1.33 ± 0.042	3.963GluIle: 3.963 ± 0.074	3.557GluLys: 3.557 ± 0.088	6.467GluLeu: 6.467 ± 0.109	1.766GluMet: 1.766 ± 0.055	2.635GluAsn: 2.635 ± 0.073	2.61GluPro: 2.61 ± 0.076	2.729GluGln: 2.729 ± 0.071	3.336GluArg: 3.336 ± 0.083	2.982GluSer: 2.982 ± 0.072	3.71GluThr: 3.71 ± 0.091	4.005GluVal: 4.005 ± 0.072	0.447GluTrp: 0.447 ± 0.028	1.886GluTyr: 1.886 ± 0.058	0.001GluXaa: 0.001 ± 0.002
Phe	3.265PheAla: 3.265 ± 0.059	0.859PheCys: 0.859 ± 0.036	2.232PheAsp: 2.232 ± 0.063	1.599PheGlu: 1.599 ± 0.053	1.558PhePhe: 1.558 ± 0.058	2.904PheGly: 2.904 ± 0.065	0.976PheHis: 0.976 ± 0.041	1.6PheIle: 1.6 ± 0.053	0.877PheLys: 0.877 ± 0.035	4.349PheLeu: 4.349 ± 0.11	0.728PheMet: 0.728 ± 0.037	1.019PheAsn: 1.019 ± 0.039	1.707PhePro: 1.707 ± 0.047	1.824PheGln: 1.824 ± 0.058	2.363PheArg: 2.363 ± 0.063	2.696PheSer: 2.696 ± 0.074	2.328PheThr: 2.328 ± 0.073	2.367PheVal: 2.367 ± 0.047	0.416PheTrp: 0.416 ± 0.023	1.434PheTyr: 1.434 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	7.069GlyAla: 7.069 ± 0.129	1.539GlyCys: 1.539 ± 0.05	4.366GlyAsp: 4.366 ± 0.098	5.232GlyGlu: 5.232 ± 0.095	3.178GlyPhe: 3.178 ± 0.07	6.832GlyGly: 6.832 ± 0.162	1.96GlyHis: 1.96 ± 0.087	4.969GlyIle: 4.969 ± 0.1	4.483GlyLys: 4.483 ± 0.089	8.091GlyLeu: 8.091 ± 0.133	2.32GlyMet: 2.32 ± 0.057	3.144GlyAsn: 3.144 ± 0.09	2.534GlyPro: 2.534 ± 0.067	3.605GlyGln: 3.605 ± 0.089	4.518GlyArg: 4.518 ± 0.098	4.958GlySer: 4.958 ± 0.097	4.502GlyThr: 4.502 ± 0.092	6.442GlyVal: 6.442 ± 0.13	0.863GlyTrp: 0.863 ± 0.04	3.138GlyTyr: 3.138 ± 0.073	0.007GlyXaa: 0.007 ± 0.003
His	1.521HisAla: 1.521 ± 0.054	0.408HisCys: 0.408 ± 0.023	0.937HisAsp: 0.937 ± 0.036	0.926HisGlu: 0.926 ± 0.043	0.861HisPhe: 0.861 ± 0.036	2.057HisGly: 2.057 ± 0.077	0.681HisHis: 0.681 ± 0.044	1.494HisIle: 1.494 ± 0.057	0.7HisLys: 0.7 ± 0.032	2.182HisLeu: 2.182 ± 0.064	0.543HisMet: 0.543 ± 0.03	0.674HisAsn: 0.674 ± 0.036	1.255HisPro: 1.255 ± 0.044	0.909HisGln: 0.909 ± 0.046	1.554HisArg: 1.554 ± 0.067	1.149HisSer: 1.149 ± 0.048	1.181HisThr: 1.181 ± 0.04	1.445HisVal: 1.445 ± 0.054	0.237HisTrp: 0.237 ± 0.018	0.678HisTyr: 0.678 ± 0.037	0.001HisXaa: 0.001 ± 0.001
Ile	4.862IleAla: 4.862 ± 0.101	1.211IleCys: 1.211 ± 0.037	2.548IleAsp: 2.548 ± 0.067	2.369IleGlu: 2.369 ± 0.058	1.999IlePhe: 1.999 ± 0.054	4.002IleGly: 4.002 ± 0.096	1.292IleHis: 1.292 ± 0.051	2.401IleIle: 2.401 ± 0.075	1.435IleLys: 1.435 ± 0.058	6.121IleLeu: 6.121 ± 0.105	1.064IleMet: 1.064 ± 0.045	1.509IleAsn: 1.509 ± 0.051	2.977IlePro: 2.977 ± 0.062	2.343IleGln: 2.343 ± 0.063	3.428IleArg: 3.428 ± 0.079	3.571IleSer: 3.571 ± 0.076	3.144IleThr: 3.144 ± 0.072	3.548IleVal: 3.548 ± 0.088	0.564IleTrp: 0.564 ± 0.028	1.687IleTyr: 1.687 ± 0.054	0.001IleXaa: 0.001 ± 0.001
Lys	3.902LysAla: 3.902 ± 0.091	0.455LysCys: 0.455 ± 0.026	1.999LysAsp: 1.999 ± 0.055	2.963LysGlu: 2.963 ± 0.075	1.137LysPhe: 1.137 ± 0.042	3.073LysGly: 3.073 ± 0.068	0.73LysHis: 0.73 ± 0.033	2.391LysIle: 2.391 ± 0.064	2.565LysLys: 2.565 ± 0.087	4.092LysLeu: 4.092 ± 0.081	1.135LysMet: 1.135 ± 0.044	1.561LysAsn: 1.561 ± 0.051	1.772LysPro: 1.772 ± 0.056	1.573LysGln: 1.573 ± 0.051	2.314LysArg: 2.314 ± 0.064	1.995LysSer: 1.995 ± 0.058	2.668LysThr: 2.668 ± 0.081	2.742LysVal: 2.742 ± 0.06	0.412LysTrp: 0.412 ± 0.027	1.419LysTyr: 1.419 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	9.729LeuAla: 9.729 ± 0.156	2.122LeuCys: 2.122 ± 0.064	5.656LeuAsp: 5.656 ± 0.094	7.036LeuGlu: 7.036 ± 0.124	3.722LeuPhe: 3.722 ± 0.088	8.023LeuGly: 8.023 ± 0.14	2.405LeuHis: 2.405 ± 0.074	4.556LeuIle: 4.556 ± 0.109	3.729LeuLys: 3.729 ± 0.092	11.423LeuLeu: 11.423 ± 0.204	2.415LeuMet: 2.415 ± 0.066	2.98LeuAsn: 2.98 ± 0.067	5.319LeuPro: 5.319 ± 0.102	4.216LeuGln: 4.216 ± 0.105	6.274LeuArg: 6.274 ± 0.129	6.377LeuSer: 6.377 ± 0.115	6.284LeuThr: 6.284 ± 0.107	6.714LeuVal: 6.714 ± 0.112	1.117LeuTrp: 1.117 ± 0.044	3.547LeuTyr: 3.547 ± 0.083	0.007LeuXaa: 0.007 ± 0.004
Met	2.704MetAla: 2.704 ± 0.066	0.301MetCys: 0.301 ± 0.021	1.677MetAsp: 1.677 ± 0.05	2.289MetGlu: 2.289 ± 0.058	0.74MetPhe: 0.74 ± 0.035	2.11MetGly: 2.11 ± 0.061	0.39MetHis: 0.39 ± 0.024	1.243MetIle: 1.243 ± 0.045	1.526MetLys: 1.526 ± 0.05	2.5MetLeu: 2.5 ± 0.072	0.701MetMet: 0.701 ± 0.039	1.061MetAsn: 1.061 ± 0.04	1.098MetPro: 1.098 ± 0.04	0.898MetGln: 0.898 ± 0.037	1.237MetArg: 1.237 ± 0.052	1.47MetSer: 1.47 ± 0.049	1.607MetThr: 1.607 ± 0.052	1.837MetVal: 1.837 ± 0.053	0.191MetTrp: 0.191 ± 0.015	0.672MetTyr: 0.672 ± 0.033	0.001MetXaa: 0.001 ± 0.001
Asn	2.862AsnAla: 2.862 ± 0.069	0.515AsnCys: 0.515 ± 0.026	1.315AsnAsp: 1.315 ± 0.05	1.604AsnGlu: 1.604 ± 0.043	1.166AsnPhe: 1.166 ± 0.038	3.145AsnGly: 3.145 ± 0.074	0.733AsnHis: 0.733 ± 0.038	1.72AsnIle: 1.72 ± 0.047	1.046AsnLys: 1.046 ± 0.039	3.746AsnLeu: 3.746 ± 0.072	0.861AsnMet: 0.861 ± 0.037	0.983AsnAsn: 0.983 ± 0.04	1.976AsnPro: 1.976 ± 0.049	1.441AsnGln: 1.441 ± 0.045	2.096AsnArg: 2.096 ± 0.06	1.597AsnSer: 1.597 ± 0.052	1.727AsnThr: 1.727 ± 0.052	2.167AsnVal: 2.167 ± 0.057	0.438AsnTrp: 0.438 ± 0.028	1.218AsnTyr: 1.218 ± 0.041	0.001AsnXaa: 0.001 ± 0.002
Pro	4.372ProAla: 4.372 ± 0.074	0.685ProCys: 0.685 ± 0.033	2.813ProAsp: 2.813 ± 0.074	4.258ProGlu: 4.258 ± 0.082	1.56ProPhe: 1.56 ± 0.053	4.132ProGly: 4.132 ± 0.103	0.841ProHis: 0.841 ± 0.038	1.919ProIle: 1.919 ± 0.053	1.717ProLys: 1.717 ± 0.053	3.827ProLeu: 3.827 ± 0.081	1.171ProMet: 1.171 ± 0.044	1.366ProAsn: 1.366 ± 0.047	1.541ProPro: 1.541 ± 0.062	1.932ProGln: 1.932 ± 0.06	1.84ProArg: 1.84 ± 0.057	2.139ProSer: 2.139 ± 0.061	2.115ProThr: 2.115 ± 0.063	4.077ProVal: 4.077 ± 0.092	0.554ProTrp: 0.554 ± 0.032	1.457ProTyr: 1.457 ± 0.04	0.001ProXaa: 0.001 ± 0.001
Gln	4.335GlnAla: 4.335 ± 0.085	0.526GlnCys: 0.526 ± 0.027	2.067GlnAsp: 2.067 ± 0.054	3.46GlnGlu: 3.46 ± 0.085	1.231GlnPhe: 1.231 ± 0.04	3.444GlnGly: 3.444 ± 0.091	0.762GlnHis: 0.762 ± 0.032	2.357GlnIle: 2.357 ± 0.058	1.992GlnLys: 1.992 ± 0.058	4.155GlnLeu: 4.155 ± 0.087	1.166GlnMet: 1.166 ± 0.045	1.581GlnAsn: 1.581 ± 0.05	1.616GlnPro: 1.616 ± 0.041	1.519GlnGln: 1.519 ± 0.056	2.242GlnArg: 2.242 ± 0.055	2.05GlnSer: 2.05 ± 0.059	2.128GlnThr: 2.128 ± 0.059	3.605GlnVal: 3.605 ± 0.089	0.435GlnTrp: 0.435 ± 0.024	1.383GlnTyr: 1.383 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	4.377ArgAla: 4.377 ± 0.094	1.036ArgCys: 1.036 ± 0.04	2.866ArgAsp: 2.866 ± 0.08	4.141ArgGlu: 4.141 ± 0.086	2.356ArgPhe: 2.356 ± 0.055	3.794ArgGly: 3.794 ± 0.08	1.236ArgHis: 1.236 ± 0.052	3.235ArgIle: 3.235 ± 0.073	2.698ArgLys: 2.698 ± 0.071	6.17ArgLeu: 6.17 ± 0.119	1.658ArgMet: 1.658 ± 0.057	2.015ArgAsn: 2.015 ± 0.053	2.414ArgPro: 2.414 ± 0.067	3.064ArgGln: 3.064 ± 0.069	4.138ArgArg: 4.138 ± 0.106	2.928ArgSer: 2.928 ± 0.073	2.737ArgThr: 2.737 ± 0.08	3.543ArgVal: 3.543 ± 0.078	0.616ArgTrp: 0.616 ± 0.032	2.262ArgTyr: 2.262 ± 0.066	0.017ArgXaa: 0.017 ± 0.005
Ser	4.856SerAla: 4.856 ± 0.091	1.078SerCys: 1.078 ± 0.037	2.684SerAsp: 2.684 ± 0.07	2.652SerGlu: 2.652 ± 0.067	2.483SerPhe: 2.483 ± 0.057	5.252SerGly: 5.252 ± 0.105	1.174SerHis: 1.174 ± 0.049	2.993SerIle: 2.993 ± 0.066	2.028SerLys: 2.028 ± 0.055	5.761SerLeu: 5.761 ± 0.1	1.64SerMet: 1.64 ± 0.052	1.747SerAsn: 1.747 ± 0.055	2.407SerPro: 2.407 ± 0.071	2.407SerGln: 2.407 ± 0.071	3.347SerArg: 3.347 ± 0.075	3.288SerSer: 3.288 ± 0.095	2.843SerThr: 2.843 ± 0.077	3.957SerVal: 3.957 ± 0.08	0.747SerTrp: 0.747 ± 0.032	2.201SerTyr: 2.201 ± 0.07	0.007SerXaa: 0.007 ± 0.003
Thr	5.699ThrAla: 5.699 ± 0.108	0.979ThrCys: 0.979 ± 0.054	2.947ThrAsp: 2.947 ± 0.076	3.444ThrGlu: 3.444 ± 0.082	2.068ThrPhe: 2.068 ± 0.061	5.492ThrGly: 5.492 ± 0.097	1.065ThrHis: 1.065 ± 0.037	2.927ThrIle: 2.927 ± 0.071	1.878ThrLys: 1.878 ± 0.069	5.494ThrLeu: 5.494 ± 0.095	1.442ThrMet: 1.442 ± 0.048	1.483ThrAsn: 1.483 ± 0.049	2.662ThrPro: 2.662 ± 0.071	1.974ThrGln: 1.974 ± 0.05	2.755ThrArg: 2.755 ± 0.075	2.755ThrSer: 2.755 ± 0.073	2.847ThrThr: 2.847 ± 0.072	5.251ThrVal: 5.251 ± 0.106	0.713ThrTrp: 0.713 ± 0.035	2.041ThrTyr: 2.041 ± 0.075	0.004ThrXaa: 0.004 ± 0.002
Val	6.258ValAla: 6.258 ± 0.11	1.535ValCys: 1.535 ± 0.049	3.953ValAsp: 3.953 ± 0.078	4.553ValGlu: 4.553 ± 0.083	2.829ValPhe: 2.829 ± 0.067	5.429ValGly: 5.429 ± 0.128	1.63ValHis: 1.63 ± 0.07	3.895ValIle: 3.895 ± 0.076	2.975ValLys: 2.975 ± 0.071	7.779ValLeu: 7.779 ± 0.13	1.778ValMet: 1.778 ± 0.05	2.37ValAsn: 2.37 ± 0.059	3.249ValPro: 3.249 ± 0.075	2.876ValGln: 2.876 ± 0.081	3.735ValArg: 3.735 ± 0.08	4.509ValSer: 4.509 ± 0.085	4.969ValThr: 4.969 ± 0.127	5.439ValVal: 5.439 ± 0.111	0.723ValTrp: 0.723 ± 0.03	2.487ValTyr: 2.487 ± 0.063	0.003ValXaa: 0.003 ± 0.002
Trp	0.957TrpAla: 0.957 ± 0.042	0.224TrpCys: 0.224 ± 0.018	0.645TrpAsp: 0.645 ± 0.037	0.824TrpGlu: 0.824 ± 0.037	0.423TrpPhe: 0.423 ± 0.028	0.887TrpGly: 0.887 ± 0.036	0.199TrpHis: 0.199 ± 0.018	0.606TrpIle: 0.606 ± 0.033	0.48TrpLys: 0.48 ± 0.027	0.979TrpLeu: 0.979 ± 0.045	0.275TrpMet: 0.275 ± 0.021	0.519TrpAsn: 0.519 ± 0.031	0.311TrpPro: 0.311 ± 0.024	0.494TrpGln: 0.494 ± 0.023	0.655TrpArg: 0.655 ± 0.032	0.645TrpSer: 0.645 ± 0.031	0.591TrpThr: 0.591 ± 0.034	0.649TrpVal: 0.649 ± 0.032	0.111TrpTrp: 0.111 ± 0.013	0.496TrpTyr: 0.496 ± 0.033	0.001TrpXaa: 0.001 ± 0.001
Tyr	3.083TyrAla: 3.083 ± 0.067	0.708TyrCys: 0.708 ± 0.032	2.2TyrAsp: 2.2 ± 0.064	2.141TyrGlu: 2.141 ± 0.054	1.477TyrPhe: 1.477 ± 0.048	2.933TyrGly: 2.933 ± 0.069	0.773TyrHis: 0.773 ± 0.03	1.693TyrIle: 1.693 ± 0.048	1.015TyrLys: 1.015 ± 0.037	3.532TyrLeu: 3.532 ± 0.074	0.765TyrMet: 0.765 ± 0.032	1.22TyrAsn: 1.22 ± 0.05	1.396TyrPro: 1.396 ± 0.05	1.481TyrGln: 1.481 ± 0.044	2.365TyrArg: 2.365 ± 0.075	1.956TyrSer: 1.956 ± 0.057	2.349TyrThr: 2.349 ± 0.08	2.37TyrVal: 2.37 ± 0.063	0.399TyrTrp: 0.399 ± 0.024	1.551TyrTyr: 1.551 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.009XaaAla: 0.009 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.003XaaGlu: 0.003 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.012XaaGly: 0.012 ± 0.004	0.001XaaHis: 0.001 ± 0.002	0.004XaaIle: 0.004 ± 0.003	0.0XaaLys: 0.0 ± 0.0	0.006XaaLeu: 0.006 ± 0.003	0.001XaaMet: 0.001 ± 0.002	0.001XaaAsn: 0.001 ± 0.001	0.012XaaPro: 0.012 ± 0.004	0.001XaaGln: 0.001 ± 0.001	0.006XaaArg: 0.006 ± 0.003	0.007XaaSer: 0.007 ± 0.003	0.001XaaThr: 0.001 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.072XaaXaa: 0.072 ± 0.013

Statistics based on 2347 proteins (691874 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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