Microbacteriaceae bacterium MWH-Ta3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; unclassified Microbacteriaceae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5X6S8|A0A2T5X6S8_9MICO Uncharacterized protein OS=Microbacteriaceae bacterium MWH-Ta3 OX=207608 GN=C8A06_0465 PE=4 SV=1
MM1 pKa = 6.87NTRR4 pKa = 11.84IVTSALLALGLVTLAGTAPAHH25 pKa = 5.97AAVGALPEE33 pKa = 4.46GQTMYY38 pKa = 10.93AISCDD43 pKa = 3.56NGPTEE48 pKa = 4.42SPVNSGTLFSIDD60 pKa = 3.66PTTAALTRR68 pKa = 11.84VGDD71 pKa = 3.85GTVGLNNSGCAGQGAVNPVDD91 pKa = 3.13GTYY94 pKa = 10.93YY95 pKa = 9.87YY96 pKa = 10.83LSWDD100 pKa = 3.62FPGVMNALAVMDD112 pKa = 5.05LEE114 pKa = 4.74TGEE117 pKa = 4.32STLVGPLSYY126 pKa = 11.11NGTPFVNSYY135 pKa = 11.11AIAIDD140 pKa = 3.64SAGNAVLTSRR150 pKa = 11.84IPGEE154 pKa = 4.02DD155 pKa = 3.29VVGLFALNLTDD166 pKa = 4.44GTTEE170 pKa = 4.77LIGTIADD177 pKa = 3.67GTNGQRR183 pKa = 11.84RR184 pKa = 11.84PYY186 pKa = 10.61ALSFDD191 pKa = 3.56NSDD194 pKa = 3.33RR195 pKa = 11.84LFAVDD200 pKa = 4.09SSNSNEE206 pKa = 3.92LLEE209 pKa = 4.42ISTVDD214 pKa = 3.36GSIVSSSTEE223 pKa = 3.72LSDD226 pKa = 4.37LGGIYY231 pKa = 10.25TMAFDD236 pKa = 5.52ADD238 pKa = 3.48NTLWMQSDD246 pKa = 4.37VEE248 pKa = 4.48LVSASITDD256 pKa = 4.02LSTRR260 pKa = 11.84SEE262 pKa = 4.1LLQFTISGYY271 pKa = 9.68PNYY274 pKa = 10.77GYY276 pKa = 8.58FTEE279 pKa = 4.96SIVIAGSGPAPTEE292 pKa = 3.81EE293 pKa = 4.71EE294 pKa = 4.13EE295 pKa = 4.56PEE297 pKa = 4.22TGGVTEE303 pKa = 5.07LADD306 pKa = 3.5TGAGNTTAIAGYY318 pKa = 9.9ALGGIALALIATRR331 pKa = 11.84TARR334 pKa = 11.84RR335 pKa = 11.84TRR337 pKa = 11.84AA338 pKa = 3.06

Molecular weight: 34.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5X7A8|A0A2T5X7A8_9MICO Uncharacterized protein OS=Microbacteriaceae bacterium MWH-Ta3 OX=207608 GN=C8A06_0660 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 9.85GRR40 pKa = 11.84KK41 pKa = 9.4SITVV45 pKa = 3.23

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1490

0

1490

484553

32

2290

325.2

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.495 ± 0.094

0.551 ± 0.013

6.178 ± 0.053

5.722 ± 0.063

3.432 ± 0.046

8.413 ± 0.049

2.164 ± 0.031

5.595 ± 0.049

2.493 ± 0.051

9.3 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.026

2.68 ± 0.032

4.729 ± 0.04

2.641 ± 0.029

6.469 ± 0.073

5.977 ± 0.056

6.4 ± 0.055

9.078 ± 0.065

1.425 ± 0.032

2.148 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski