Macaca mulatta feces associated virus 3
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PZP4|A0A1W5PZP4_9VIRU Cap OS=Macaca mulatta feces associated virus 3 OX=2499225 PE=4 SV=1
MM1 pKa = 8.15 IMFVKK6 pKa = 10.62 VSEE9 pKa = 4.46 TYY11 pKa = 10.75 DD12 pKa = 3.33 LSTKK16 pKa = 8.75 VGKK19 pKa = 9.33 MGLVGIHH26 pKa = 5.42 TPEE29 pKa = 3.73 GRR31 pKa = 11.84 LVYY34 pKa = 10.03 KK35 pKa = 10.33 HH36 pKa = 6.18 WEE38 pKa = 4.08 GLYY41 pKa = 10.0 KK42 pKa = 10.45 NYY44 pKa = 10.15 RR45 pKa = 11.84 RR46 pKa = 11.84 VRR48 pKa = 11.84 FASCDD53 pKa = 3.32 VAMACASMLPADD65 pKa = 4.26 PLQIGVEE72 pKa = 4.06 AGSIAPQDD80 pKa = 3.62 MFNPILYY87 pKa = 8.86 KK88 pKa = 10.64 ACSNDD93 pKa = 3.3 SMSQILNRR101 pKa = 11.84 IYY103 pKa = 10.9 AGSTATNVDD112 pKa = 3.35 VAKK115 pKa = 10.97 NSIIASNEE123 pKa = 3.48 ASFGNAGVVVGEE135 pKa = 4.74 HH136 pKa = 5.86 GAEE139 pKa = 4.15 SVSNTPSQFDD149 pKa = 2.98 IYY151 pKa = 11.06 YY152 pKa = 11.08 ALLSDD157 pKa = 3.58 TNGWKK162 pKa = 10.23 KK163 pKa = 10.97 AMPQAGLQMRR173 pKa = 11.84 GLYY176 pKa = 9.32 PLVWSVVKK184 pKa = 9.75 TLGQPSNWSSSIQPEE199 pKa = 3.93 IVVPGTNEE207 pKa = 3.53 QTGATNYY214 pKa = 10.61 GEE216 pKa = 5.11 SNTSLGAMGQYY227 pKa = 9.33 MRR229 pKa = 11.84 GPAMRR234 pKa = 11.84 MPACDD239 pKa = 3.21 TYY241 pKa = 11.66 VATNMDD247 pKa = 3.68 GGQTVNVDD255 pKa = 3.05 NVDD258 pKa = 3.64 LVKK261 pKa = 10.65 IRR263 pKa = 11.84 DD264 pKa = 3.59 NDD266 pKa = 3.57 KK267 pKa = 11.07 AYY269 pKa = 7.53 PTQNSIDD276 pKa = 3.74 KK277 pKa = 9.18 FFPSPIANFAKK288 pKa = 10.5 PNLDD292 pKa = 4.29 LPDD295 pKa = 4.1 CFVGVMILPPATLNQLYY312 pKa = 10.19 YY313 pKa = 10.14 RR314 pKa = 11.84 LKK316 pKa = 9.08 VTWTVEE322 pKa = 3.97 FSEE325 pKa = 4.29 PRR327 pKa = 11.84 SLTEE331 pKa = 3.44 LTNWYY336 pKa = 9.52 GLQSTGIQSYY346 pKa = 8.11 ATDD349 pKa = 3.61 YY350 pKa = 9.2 ATQTQALKK358 pKa = 10.89 NSGSSVTLGMLDD370 pKa = 3.4 TSEE373 pKa = 3.91 VDD375 pKa = 2.88 AVKK378 pKa = 10.53 IMEE381 pKa = 4.44 GNN383 pKa = 3.24
Molecular weight: 41.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.893
IPC2_protein 5.067
IPC_protein 4.991
Toseland 4.876
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.105
Wikipedia 4.851
Rodwell 4.863
Grimsley 4.787
Solomon 4.965
Lehninger 4.927
Nozaki 5.08
DTASelect 5.27
Thurlkill 4.876
EMBOSS 4.876
Sillero 5.143
Patrickios 3.503
IPC_peptide 4.965
IPC2_peptide 5.13
IPC2.peptide.svr19 5.142
Protein with the highest isoelectric point:
>tr|A0A1W5PZP4|A0A1W5PZP4_9VIRU Cap OS=Macaca mulatta feces associated virus 3 OX=2499225 PE=4 SV=1
MM1 pKa = 7.68 TSPQKK6 pKa = 10.37 LEE8 pKa = 3.32 KK9 pKa = 9.47 WAWLVSILQKK19 pKa = 10.77 EE20 pKa = 4.83 GWFTNTGKK28 pKa = 10.43 DD29 pKa = 3.15 STKK32 pKa = 10.02 IIVVFVSLLAMLLWLVPRR50 pKa = 11.84 CCLPILSRR58 pKa = 11.84 SVLKK62 pKa = 10.43 LVRR65 pKa = 11.84 SRR67 pKa = 11.84 PRR69 pKa = 11.84 TCLIRR74 pKa = 11.84 SCTKK78 pKa = 9.74 PVPTTRR84 pKa = 11.84 CLRR87 pKa = 11.84 SSTEE91 pKa = 3.84 SMLDD95 pKa = 3.41 LPQQMWMSPRR105 pKa = 11.84 TRR107 pKa = 11.84 SLLLMKK113 pKa = 10.57 LLLGTLVWLSVNTVLSLL130 pKa = 3.7
Molecular weight: 14.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.545
IPC_protein 9.911
Toseland 10.965
ProMoST 10.599
Dawson 11.008
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.301
Grimsley 11.023
Solomon 11.169
Lehninger 11.14
Nozaki 10.965
DTASelect 10.672
Thurlkill 10.935
EMBOSS 11.374
Sillero 10.95
Patrickios 11.067
IPC_peptide 11.184
IPC2_peptide 9.97
IPC2.peptide.svr19 8.784
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
712
130
383
237.3
26.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.618 ± 1.772
1.826 ± 0.592
4.213 ± 0.859
4.213 ± 0.787
1.966 ± 0.467
7.444 ± 1.588
2.388 ± 1.835
4.635 ± 0.242
4.916 ± 0.401
9.27 ± 3.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.652 ± 0.846
4.354 ± 1.523
5.056 ± 0.618
5.197 ± 1.148
8.146 ± 4.345
8.006 ± 1.494
6.742 ± 0.983
8.006 ± 0.199
1.685 ± 0.938
2.669 ± 1.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here