Amino acid dipepetide frequency for Cafeteria roenbergensis (Marine flagellate)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	39.544AlaAla: 39.544 ± 0.439	1.856AlaCys: 1.856 ± 0.026	9.031AlaAsp: 9.031 ± 0.088	10.437AlaGlu: 10.437 ± 0.133	3.804AlaPhe: 3.804 ± 0.032	16.401AlaGly: 16.401 ± 0.128	2.437AlaHis: 2.437 ± 0.026	3.702AlaIle: 3.702 ± 0.032	4.559AlaLys: 4.559 ± 0.07	13.588AlaLeu: 13.588 ± 0.088	3.372AlaMet: 3.372 ± 0.029	2.15AlaAsn: 2.15 ± 0.027	8.725AlaPro: 8.725 ± 0.071	4.109AlaGln: 4.109 ± 0.034	10.984AlaArg: 10.984 ± 0.091	14.574AlaSer: 14.574 ± 0.1	8.01AlaThr: 8.01 ± 0.059	11.674AlaVal: 11.674 ± 0.105	1.871AlaTrp: 1.871 ± 0.026	1.564AlaTyr: 1.564 ± 0.023	0.0AlaXaa: 0.0 ± 0.0
Cys	1.52CysAla: 1.52 ± 0.022	0.348CysCys: 0.348 ± 0.011	0.693CysAsp: 0.693 ± 0.013	0.619CysGlu: 0.619 ± 0.011	0.423CysPhe: 0.423 ± 0.01	1.245CysGly: 1.245 ± 0.021	0.243CysHis: 0.243 ± 0.008	0.41CysIle: 0.41 ± 0.01	0.373CysLys: 0.373 ± 0.009	1.164CysLeu: 1.164 ± 0.018	0.251CysMet: 0.251 ± 0.007	0.285CysAsn: 0.285 ± 0.01	0.799CysPro: 0.799 ± 0.027	0.319CysGln: 0.319 ± 0.008	0.886CysArg: 0.886 ± 0.016	1.023CysSer: 1.023 ± 0.02	0.625CysThr: 0.625 ± 0.013	0.979CysVal: 0.979 ± 0.021	0.18CysTrp: 0.18 ± 0.006	0.182CysTyr: 0.182 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	9.725AspAla: 9.725 ± 0.078	0.65AspCys: 0.65 ± 0.014	5.326AspAsp: 5.326 ± 0.092	4.477AspGlu: 4.477 ± 0.057	1.452AspPhe: 1.452 ± 0.022	6.188AspGly: 6.188 ± 0.045	0.882AspHis: 0.882 ± 0.018	1.485AspIle: 1.485 ± 0.021	1.366AspLys: 1.366 ± 0.028	4.032AspLeu: 4.032 ± 0.033	1.019AspMet: 1.019 ± 0.018	0.829AspAsn: 0.829 ± 0.016	3.325AspPro: 3.325 ± 0.029	1.102AspGln: 1.102 ± 0.014	3.129AspArg: 3.129 ± 0.032	3.994AspSer: 3.994 ± 0.032	2.324AspThr: 2.324 ± 0.025	4.25AspVal: 4.25 ± 0.052	0.711AspTrp: 0.711 ± 0.012	0.69AspTyr: 0.69 ± 0.014	0.0AspXaa: 0.0 ± 0.0
Glu	11.754GluAla: 11.754 ± 0.145	0.607GluCys: 0.607 ± 0.012	3.865GluAsp: 3.865 ± 0.044	5.158GluGlu: 5.158 ± 0.111	1.339GluPhe: 1.339 ± 0.021	4.818GluGly: 4.818 ± 0.041	1.153GluHis: 1.153 ± 0.019	1.112GluIle: 1.112 ± 0.016	1.21GluLys: 1.21 ± 0.026	5.355GluLeu: 5.355 ± 0.044	1.023GluMet: 1.023 ± 0.017	0.724GluAsn: 0.724 ± 0.016	2.754GluPro: 2.754 ± 0.029	1.973GluGln: 1.973 ± 0.026	4.469GluArg: 4.469 ± 0.051	3.414GluSer: 3.414 ± 0.029	2.365GluThr: 2.365 ± 0.023	3.592GluVal: 3.592 ± 0.047	0.732GluTrp: 0.732 ± 0.013	0.718GluTyr: 0.718 ± 0.013	0.0GluXaa: 0.0 ± 0.0
Phe	3.782PheAla: 3.782 ± 0.038	0.417PheCys: 0.417 ± 0.01	1.77PheAsp: 1.77 ± 0.023	1.616PheGlu: 1.616 ± 0.025	0.981PhePhe: 0.981 ± 0.018	2.733PheGly: 2.733 ± 0.034	0.546PheHis: 0.546 ± 0.011	0.719PheIle: 0.719 ± 0.015	0.726PheLys: 0.726 ± 0.016	2.276PheLeu: 2.276 ± 0.03	0.487PheMet: 0.487 ± 0.011	0.633PheAsn: 0.633 ± 0.013	1.262PhePro: 1.262 ± 0.018	0.712PheGln: 0.712 ± 0.013	1.915PheArg: 1.915 ± 0.022	1.999PheSer: 1.999 ± 0.024	1.34PheThr: 1.34 ± 0.022	2.372PheVal: 2.372 ± 0.026	0.36PheTrp: 0.36 ± 0.008	0.457PheTyr: 0.457 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	15.136GlyAla: 15.136 ± 0.104	1.104GlyCys: 1.104 ± 0.021	5.311GlyAsp: 5.311 ± 0.058	4.764GlyGlu: 4.764 ± 0.04	2.522GlyPhe: 2.522 ± 0.028	11.763GlyGly: 11.763 ± 0.131	1.901GlyHis: 1.901 ± 0.023	2.304GlyIle: 2.304 ± 0.023	2.672GlyLys: 2.672 ± 0.033	7.084GlyLeu: 7.084 ± 0.042	1.808GlyMet: 1.808 ± 0.026	1.575GlyAsn: 1.575 ± 0.028	5.022GlyPro: 5.022 ± 0.046	2.546GlyGln: 2.546 ± 0.036	6.649GlyArg: 6.649 ± 0.051	8.934GlySer: 8.934 ± 0.092	4.727GlyThr: 4.727 ± 0.068	6.206GlyVal: 6.206 ± 0.077	1.251GlyTrp: 1.251 ± 0.016	1.295GlyTyr: 1.295 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	2.655HisAla: 2.655 ± 0.032	0.302HisCys: 0.302 ± 0.008	0.981HisAsp: 0.981 ± 0.018	0.961HisGlu: 0.961 ± 0.013	0.561HisPhe: 0.561 ± 0.011	1.957HisGly: 1.957 ± 0.027	0.497HisHis: 0.497 ± 0.015	0.524HisIle: 0.524 ± 0.01	0.495HisLys: 0.495 ± 0.012	1.546HisLeu: 1.546 ± 0.018	0.36HisMet: 0.36 ± 0.008	0.34HisAsn: 0.34 ± 0.008	1.266HisPro: 1.266 ± 0.018	0.487HisGln: 0.487 ± 0.01	1.482HisArg: 1.482 ± 0.02	1.402HisSer: 1.402 ± 0.015	0.864HisThr: 0.864 ± 0.013	1.381HisVal: 1.381 ± 0.017	0.26HisTrp: 0.26 ± 0.007	0.307HisTyr: 0.307 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.582IleAla: 3.582 ± 0.029	0.326IleCys: 0.326 ± 0.007	1.56IleAsp: 1.56 ± 0.022	1.492IleGlu: 1.492 ± 0.023	0.738IlePhe: 0.738 ± 0.014	2.043IleGly: 2.043 ± 0.025	0.477IleHis: 0.477 ± 0.009	0.802IleIle: 0.802 ± 0.014	0.8IleLys: 0.8 ± 0.016	1.947IleLeu: 1.947 ± 0.027	0.491IleMet: 0.491 ± 0.011	0.597IleAsn: 0.597 ± 0.014	1.27IlePro: 1.27 ± 0.015	0.71IleGln: 0.71 ± 0.014	1.81IleArg: 1.81 ± 0.018	1.609IleSer: 1.609 ± 0.02	1.351IleThr: 1.351 ± 0.022	2.179IleVal: 2.179 ± 0.025	0.245IleTrp: 0.245 ± 0.007	0.366IleTyr: 0.366 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	4.339LysAla: 4.339 ± 0.067	0.272LysCys: 0.272 ± 0.007	1.454LysAsp: 1.454 ± 0.026	1.56LysGlu: 1.56 ± 0.031	0.645LysPhe: 0.645 ± 0.014	2.004LysGly: 2.004 ± 0.032	0.625LysHis: 0.625 ± 0.013	0.595LysIle: 0.595 ± 0.014	0.994LysLys: 0.994 ± 0.034	2.365LysLeu: 2.365 ± 0.032	0.442LysMet: 0.442 ± 0.011	0.445LysAsn: 0.445 ± 0.013	1.677LysPro: 1.677 ± 0.033	1.01LysGln: 1.01 ± 0.025	2.374LysArg: 2.374 ± 0.034	1.61LysSer: 1.61 ± 0.023	1.215LysThr: 1.215 ± 0.022	1.523LysVal: 1.523 ± 0.026	0.318LysTrp: 0.318 ± 0.008	0.426LysTyr: 0.426 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	13.308LeuAla: 13.308 ± 0.086	1.261LeuCys: 1.261 ± 0.018	4.781LeuAsp: 4.781 ± 0.038	5.026LeuGlu: 5.026 ± 0.05	2.497LeuPhe: 2.497 ± 0.029	7.083LeuGly: 7.083 ± 0.054	1.76LeuHis: 1.76 ± 0.021	1.891LeuIle: 1.891 ± 0.026	2.043LeuLys: 2.043 ± 0.03	8.884LeuLeu: 8.884 ± 0.069	1.477LeuMet: 1.477 ± 0.02	1.452LeuAsn: 1.452 ± 0.021	5.638LeuPro: 5.638 ± 0.044	2.909LeuGln: 2.909 ± 0.027	7.73LeuArg: 7.73 ± 0.053	6.563LeuSer: 6.563 ± 0.046	4.062LeuThr: 4.062 ± 0.037	6.622LeuVal: 6.622 ± 0.054	1.132LeuTrp: 1.132 ± 0.017	1.16LeuTyr: 1.16 ± 0.017	0.0LeuXaa: 0.0 ± 0.0
Met	3.169MetAla: 3.169 ± 0.031	0.226MetCys: 0.226 ± 0.005	1.018MetAsp: 1.018 ± 0.017	1.004MetGlu: 1.004 ± 0.016	0.476MetPhe: 0.476 ± 0.01	1.546MetGly: 1.546 ± 0.022	0.414MetHis: 0.414 ± 0.009	0.407MetIle: 0.407 ± 0.009	0.386MetLys: 0.386 ± 0.011	2.021MetLeu: 2.021 ± 0.021	0.393MetMet: 0.393 ± 0.01	0.323MetAsn: 0.323 ± 0.009	1.21MetPro: 1.21 ± 0.019	0.696MetGln: 0.696 ± 0.011	1.669MetArg: 1.669 ± 0.02	1.331MetSer: 1.331 ± 0.016	0.924MetThr: 0.924 ± 0.014	1.255MetVal: 1.255 ± 0.019	0.231MetTrp: 0.231 ± 0.007	0.258MetTyr: 0.258 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.281AsnAla: 2.281 ± 0.026	0.254AsnCys: 0.254 ± 0.008	0.865AsnAsp: 0.865 ± 0.016	0.809AsnGlu: 0.809 ± 0.018	0.56AsnPhe: 0.56 ± 0.015	1.567AsnGly: 1.567 ± 0.03	0.291AsnHis: 0.291 ± 0.008	0.588AsnIle: 0.588 ± 0.012	0.542AsnLys: 0.542 ± 0.014	1.285AsnLeu: 1.285 ± 0.021	0.329AsnMet: 0.329 ± 0.009	0.384AsnAsn: 0.384 ± 0.01	1.136AsnPro: 1.136 ± 0.016	0.408AsnGln: 0.408 ± 0.01	1.044AsnArg: 1.044 ± 0.017	1.111AsnSer: 1.111 ± 0.021	0.831AsnThr: 0.831 ± 0.015	1.304AsnVal: 1.304 ± 0.022	0.243AsnTrp: 0.243 ± 0.007	0.304AsnTyr: 0.304 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	10.124ProAla: 10.124 ± 0.078	0.588ProCys: 0.588 ± 0.015	3.101ProAsp: 3.101 ± 0.038	3.285ProGlu: 3.285 ± 0.026	1.539ProPhe: 1.539 ± 0.019	5.824ProGly: 5.824 ± 0.058	1.067ProHis: 1.067 ± 0.018	1.177ProIle: 1.177 ± 0.016	1.332ProLys: 1.332 ± 0.025	4.747ProLeu: 4.747 ± 0.041	0.951ProMet: 0.951 ± 0.014	0.79ProAsn: 0.79 ± 0.014	4.605ProPro: 4.605 ± 0.053	1.473ProGln: 1.473 ± 0.02	3.762ProArg: 3.762 ± 0.033	5.542ProSer: 5.542 ± 0.054	2.896ProThr: 2.896 ± 0.03	4.052ProVal: 4.052 ± 0.038	0.741ProTrp: 0.741 ± 0.013	0.649ProTyr: 0.649 ± 0.014	0.0ProXaa: 0.0 ± 0.0
Gln	4.773GlnAla: 4.773 ± 0.043	0.397GlnCys: 0.397 ± 0.012	1.221GlnAsp: 1.221 ± 0.018	1.493GlnGlu: 1.493 ± 0.023	0.721GlnPhe: 0.721 ± 0.013	2.208GlnGly: 2.208 ± 0.021	0.638GlnHis: 0.638 ± 0.012	0.606GlnIle: 0.606 ± 0.012	0.555GlnLys: 0.555 ± 0.022	3.114GlnLeu: 3.114 ± 0.029	0.537GlnMet: 0.537 ± 0.012	0.396GlnAsn: 0.396 ± 0.01	1.757GlnPro: 1.757 ± 0.02	1.323GlnGln: 1.323 ± 0.027	2.657GlnArg: 2.657 ± 0.032	1.95GlnSer: 1.95 ± 0.022	1.214GlnThr: 1.214 ± 0.017	1.83GlnVal: 1.83 ± 0.028	0.439GlnTrp: 0.439 ± 0.009	0.416GlnTyr: 0.416 ± 0.01	0.0GlnXaa: 0.0 ± 0.0
Arg	11.28ArgAla: 11.28 ± 0.097	0.995ArgCys: 0.995 ± 0.016	3.481ArgAsp: 3.481 ± 0.032	3.999ArgGlu: 3.999 ± 0.046	2.224ArgPhe: 2.224 ± 0.022	6.124ArgGly: 6.124 ± 0.045	1.596ArgHis: 1.596 ± 0.02	2.103ArgIle: 2.103 ± 0.021	2.075ArgLys: 2.075 ± 0.029	7.201ArgLeu: 7.201 ± 0.057	1.53ArgMet: 1.53 ± 0.022	1.179ArgAsn: 1.179 ± 0.015	4.043ArgPro: 4.043 ± 0.036	2.433ArgGln: 2.433 ± 0.028	7.392ArgArg: 7.392 ± 0.078	5.548ArgSer: 5.548 ± 0.044	3.164ArgThr: 3.164 ± 0.029	5.431ArgVal: 5.431 ± 0.046	1.051ArgTrp: 1.051 ± 0.016	0.978ArgTyr: 0.978 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	13.157SerAla: 13.157 ± 0.097	0.889SerCys: 0.889 ± 0.017	4.349SerAsp: 4.349 ± 0.035	3.737SerGlu: 3.737 ± 0.035	2.065SerPhe: 2.065 ± 0.029	8.623SerGly: 8.623 ± 0.091	1.373SerHis: 1.373 ± 0.018	1.969SerIle: 1.969 ± 0.025	2.129SerLys: 2.129 ± 0.027	6.948SerLeu: 6.948 ± 0.051	1.483SerMet: 1.483 ± 0.018	1.25SerAsn: 1.25 ± 0.024	5.025SerPro: 5.025 ± 0.054	2.01SerGln: 2.01 ± 0.022	5.439SerArg: 5.439 ± 0.041	8.582SerSer: 8.582 ± 0.093	3.95SerThr: 3.95 ± 0.038	5.7SerVal: 5.7 ± 0.063	0.941SerTrp: 0.941 ± 0.016	0.89SerTyr: 0.89 ± 0.018	0.0SerXaa: 0.0 ± 0.0
Thr	7.801ThrAla: 7.801 ± 0.051	0.618ThrCys: 0.618 ± 0.016	2.509ThrAsp: 2.509 ± 0.03	2.295ThrGlu: 2.295 ± 0.025	1.39ThrPhe: 1.39 ± 0.027	4.667ThrGly: 4.667 ± 0.046	0.841ThrHis: 0.841 ± 0.015	1.365ThrIle: 1.365 ± 0.021	1.202ThrLys: 1.202 ± 0.02	4.43ThrLeu: 4.43 ± 0.052	0.98ThrMet: 0.98 ± 0.016	0.804ThrAsn: 0.804 ± 0.017	3.135ThrPro: 3.135 ± 0.04	1.142ThrGln: 1.142 ± 0.016	2.914ThrArg: 2.914 ± 0.028	3.914ThrSer: 3.914 ± 0.043	2.513ThrThr: 2.513 ± 0.035	4.088ThrVal: 4.088 ± 0.058	0.582ThrTrp: 0.582 ± 0.013	0.677ThrTyr: 0.677 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	11.264ValAla: 11.264 ± 0.126	1.033ValCys: 1.033 ± 0.018	4.139ValAsp: 4.139 ± 0.043	3.979ValGlu: 3.979 ± 0.034	2.261ValPhe: 2.261 ± 0.023	5.541ValGly: 5.541 ± 0.055	1.29ValHis: 1.29 ± 0.017	1.931ValIle: 1.931 ± 0.026	1.73ValLys: 1.73 ± 0.028	6.837ValLeu: 6.837 ± 0.05	1.471ValMet: 1.471 ± 0.018	1.324ValAsn: 1.324 ± 0.025	4.383ValPro: 4.383 ± 0.048	2.058ValGln: 2.058 ± 0.022	5.443ValArg: 5.443 ± 0.042	5.476ValSer: 5.476 ± 0.072	4.067ValThr: 4.067 ± 0.068	6.382ValVal: 6.382 ± 0.063	0.984ValTrp: 0.984 ± 0.016	1.055ValTyr: 1.055 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.714TrpAla: 1.714 ± 0.019	0.196TrpCys: 0.196 ± 0.006	0.681TrpAsp: 0.681 ± 0.013	0.708TrpGlu: 0.708 ± 0.012	0.384TrpPhe: 0.384 ± 0.009	0.939TrpGly: 0.939 ± 0.016	0.312TrpHis: 0.312 ± 0.008	0.307TrpIle: 0.307 ± 0.009	0.31TrpLys: 0.31 ± 0.009	1.339TrpLeu: 1.339 ± 0.02	0.264TrpMet: 0.264 ± 0.007	0.277TrpAsn: 0.277 ± 0.007	0.659TrpPro: 0.659 ± 0.012	0.466TrpGln: 0.466 ± 0.01	1.147TrpArg: 1.147 ± 0.016	0.955TrpSer: 0.955 ± 0.018	0.719TrpThr: 0.719 ± 0.014	0.878TrpVal: 0.878 ± 0.014	0.243TrpTrp: 0.243 ± 0.007	0.216TrpTyr: 0.216 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.482TyrAla: 1.482 ± 0.019	0.23TyrCys: 0.23 ± 0.01	0.82TyrAsp: 0.82 ± 0.016	0.72TyrGlu: 0.72 ± 0.014	0.523TyrPhe: 0.523 ± 0.011	1.299TyrGly: 1.299 ± 0.022	0.282TyrHis: 0.282 ± 0.008	0.396TyrIle: 0.396 ± 0.009	0.393TyrLys: 0.393 ± 0.012	1.115TyrLeu: 1.115 ± 0.023	0.294TyrMet: 0.294 ± 0.009	0.377TyrAsn: 0.377 ± 0.01	0.558TyrPro: 0.558 ± 0.011	0.357TyrGln: 0.357 ± 0.011	0.932TyrArg: 0.932 ± 0.013	0.976TyrSer: 0.976 ± 0.018	0.704TyrThr: 0.704 ± 0.015	0.951TyrVal: 0.951 ± 0.02	0.198TyrTrp: 0.198 ± 0.007	0.322TyrTyr: 0.322 ± 0.01	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8530 proteins (5899624 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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