Amino acid dipepetide frequency for Enterobacter phage E-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.308AlaAla: 10.308 ± 1.324
0.531AlaCys: 0.531 ± 0.275
5.739AlaAsp: 5.739 ± 0.772
5.845AlaGlu: 5.845 ± 0.891
3.507AlaPhe: 3.507 ± 0.662
7.651AlaGly: 7.651 ± 1.107
1.488AlaHis: 1.488 ± 0.381
5.207AlaIle: 5.207 ± 0.555
6.801AlaLys: 6.801 ± 0.998
7.758AlaLeu: 7.758 ± 0.902
2.976AlaMet: 2.976 ± 0.72
4.995AlaAsn: 4.995 ± 0.742
2.444AlaPro: 2.444 ± 0.47
3.507AlaGln: 3.507 ± 0.703
5.313AlaArg: 5.313 ± 0.839
5.845AlaSer: 5.845 ± 1.119
4.782AlaThr: 4.782 ± 0.883
5.739AlaVal: 5.739 ± 0.696
1.594AlaTrp: 1.594 ± 0.496
2.019AlaTyr: 2.019 ± 0.497
0.0AlaXaa: 0.0 ± 0.0
Cys
0.531CysAla: 0.531 ± 0.211
0.106CysCys: 0.106 ± 0.106
0.638CysAsp: 0.638 ± 0.234
0.425CysGlu: 0.425 ± 0.226
0.213CysPhe: 0.213 ± 0.25
0.531CysGly: 0.531 ± 0.242
0.425CysHis: 0.425 ± 0.268
0.531CysIle: 0.531 ± 0.245
0.531CysLys: 0.531 ± 0.286
0.744CysLeu: 0.744 ± 0.291
0.106CysMet: 0.106 ± 0.115
0.106CysAsn: 0.106 ± 0.115
0.213CysPro: 0.213 ± 0.157
0.744CysGln: 0.744 ± 0.292
0.425CysArg: 0.425 ± 0.324
0.319CysSer: 0.319 ± 0.208
0.106CysThr: 0.106 ± 0.099
0.213CysVal: 0.213 ± 0.167
0.213CysTrp: 0.213 ± 0.157
0.213CysTyr: 0.213 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
7.014AspAla: 7.014 ± 0.737
0.213AspCys: 0.213 ± 0.144
4.357AspAsp: 4.357 ± 0.679
4.782AspGlu: 4.782 ± 0.83
2.869AspPhe: 2.869 ± 0.589
6.27AspGly: 6.27 ± 0.879
0.956AspHis: 0.956 ± 0.283
2.444AspIle: 2.444 ± 0.538
4.038AspLys: 4.038 ± 0.845
4.463AspLeu: 4.463 ± 0.86
2.657AspMet: 2.657 ± 0.636
3.082AspAsn: 3.082 ± 0.389
3.188AspPro: 3.188 ± 0.488
2.019AspGln: 2.019 ± 0.497
3.294AspArg: 3.294 ± 0.661
2.763AspSer: 2.763 ± 0.752
3.932AspThr: 3.932 ± 0.589
4.251AspVal: 4.251 ± 0.578
0.85AspTrp: 0.85 ± 0.381
1.913AspTyr: 1.913 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
7.651GluAla: 7.651 ± 1.102
0.638GluCys: 0.638 ± 0.245
5.313GluAsp: 5.313 ± 0.843
4.995GluGlu: 4.995 ± 0.874
2.763GluPhe: 2.763 ± 0.488
5.526GluGly: 5.526 ± 0.653
1.594GluHis: 1.594 ± 0.47
3.294GluIle: 3.294 ± 0.537
3.719GluLys: 3.719 ± 0.882
6.057GluLeu: 6.057 ± 0.712
2.976GluMet: 2.976 ± 0.642
2.125GluAsn: 2.125 ± 0.425
2.019GluPro: 2.019 ± 0.43
3.826GluGln: 3.826 ± 0.885
3.613GluArg: 3.613 ± 0.637
4.251GluSer: 4.251 ± 0.697
3.082GluThr: 3.082 ± 0.518
4.038GluVal: 4.038 ± 0.581
1.063GluTrp: 1.063 ± 0.363
3.188GluTyr: 3.188 ± 0.463
0.0GluXaa: 0.0 ± 0.0
Phe
2.657PheAla: 2.657 ± 0.436
0.106PheCys: 0.106 ± 0.109
3.188PheAsp: 3.188 ± 0.721
2.019PheGlu: 2.019 ± 0.388
1.169PhePhe: 1.169 ± 0.355
3.188PheGly: 3.188 ± 0.719
0.425PheHis: 0.425 ± 0.255
1.913PheIle: 1.913 ± 0.57
2.763PheLys: 2.763 ± 0.63
3.082PheLeu: 3.082 ± 0.709
1.063PheMet: 1.063 ± 0.339
2.232PheAsn: 2.232 ± 0.468
1.382PhePro: 1.382 ± 0.488
1.488PheGln: 1.488 ± 0.419
1.913PheArg: 1.913 ± 0.386
1.594PheSer: 1.594 ± 0.421
2.869PheThr: 2.869 ± 0.591
2.019PheVal: 2.019 ± 0.384
0.319PheTrp: 0.319 ± 0.186
0.531PheTyr: 0.531 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
7.545GlyAla: 7.545 ± 0.857
0.638GlyCys: 0.638 ± 0.337
5.739GlyAsp: 5.739 ± 0.661
5.42GlyGlu: 5.42 ± 0.844
3.294GlyPhe: 3.294 ± 0.47
6.482GlyGly: 6.482 ± 1.275
0.956GlyHis: 0.956 ± 0.256
4.995GlyIle: 4.995 ± 0.752
5.951GlyLys: 5.951 ± 0.88
6.589GlyLeu: 6.589 ± 1.089
3.188GlyMet: 3.188 ± 0.488
3.719GlyAsn: 3.719 ± 0.99
0.638GlyPro: 0.638 ± 0.188
2.869GlyGln: 2.869 ± 0.544
4.251GlyArg: 4.251 ± 0.515
4.251GlySer: 4.251 ± 0.678
3.082GlyThr: 3.082 ± 0.924
4.676GlyVal: 4.676 ± 0.514
1.7GlyTrp: 1.7 ± 0.494
2.55GlyTyr: 2.55 ± 0.583
0.0GlyXaa: 0.0 ± 0.0
His
1.488HisAla: 1.488 ± 0.44
0.213HisCys: 0.213 ± 0.178
0.531HisAsp: 0.531 ± 0.281
1.063HisGlu: 1.063 ± 0.391
0.531HisPhe: 0.531 ± 0.227
1.382HisGly: 1.382 ± 0.38
0.425HisHis: 0.425 ± 0.249
1.488HisIle: 1.488 ± 0.346
0.956HisLys: 0.956 ± 0.323
2.444HisLeu: 2.444 ± 0.513
0.213HisMet: 0.213 ± 0.132
0.638HisAsn: 0.638 ± 0.199
0.638HisPro: 0.638 ± 0.241
0.0HisGln: 0.0 ± 0.0
0.638HisArg: 0.638 ± 0.252
1.382HisSer: 1.382 ± 0.496
0.85HisThr: 0.85 ± 0.222
1.275HisVal: 1.275 ± 0.331
0.319HisTrp: 0.319 ± 0.191
0.744HisTyr: 0.744 ± 0.278
0.0HisXaa: 0.0 ± 0.0
Ile
3.507IleAla: 3.507 ± 0.558
0.319IleCys: 0.319 ± 0.163
2.976IleAsp: 2.976 ± 0.78
4.357IleGlu: 4.357 ± 0.678
0.956IlePhe: 0.956 ± 0.276
3.507IleGly: 3.507 ± 0.694
1.169IleHis: 1.169 ± 0.391
2.869IleIle: 2.869 ± 0.526
4.038IleLys: 4.038 ± 0.547
4.038IleLeu: 4.038 ± 0.601
1.169IleMet: 1.169 ± 0.368
2.763IleAsn: 2.763 ± 0.929
2.444IlePro: 2.444 ± 0.641
2.019IleGln: 2.019 ± 0.437
3.613IleArg: 3.613 ± 0.705
3.188IleSer: 3.188 ± 0.594
2.763IleThr: 2.763 ± 0.684
3.294IleVal: 3.294 ± 0.552
0.425IleTrp: 0.425 ± 0.189
2.019IleTyr: 2.019 ± 0.372
0.0IleXaa: 0.0 ± 0.0
Lys
6.908LysAla: 6.908 ± 1.065
0.531LysCys: 0.531 ± 0.268
3.294LysAsp: 3.294 ± 0.571
5.526LysGlu: 5.526 ± 0.712
2.55LysPhe: 2.55 ± 0.473
4.782LysGly: 4.782 ± 0.729
1.169LysHis: 1.169 ± 0.392
1.807LysIle: 1.807 ± 0.368
4.145LysLys: 4.145 ± 1.086
5.313LysLeu: 5.313 ± 1.008
1.807LysMet: 1.807 ± 0.423
2.338LysAsn: 2.338 ± 0.435
2.55LysPro: 2.55 ± 0.598
3.082LysGln: 3.082 ± 0.687
3.613LysArg: 3.613 ± 0.604
3.826LysSer: 3.826 ± 0.873
2.976LysThr: 2.976 ± 0.393
5.42LysVal: 5.42 ± 0.638
0.638LysTrp: 0.638 ± 0.264
2.232LysTyr: 2.232 ± 0.427
0.0LysXaa: 0.0 ± 0.0
Leu
6.482LeuAla: 6.482 ± 0.785
0.213LeuCys: 0.213 ± 0.156
4.995LeuAsp: 4.995 ± 0.535
6.376LeuGlu: 6.376 ± 1.089
2.976LeuPhe: 2.976 ± 0.618
4.145LeuGly: 4.145 ± 0.595
1.488LeuHis: 1.488 ± 0.475
4.251LeuIle: 4.251 ± 0.602
5.951LeuLys: 5.951 ± 0.961
4.676LeuLeu: 4.676 ± 0.788
2.338LeuMet: 2.338 ± 0.524
4.145LeuAsn: 4.145 ± 0.724
3.507LeuPro: 3.507 ± 0.659
3.507LeuGln: 3.507 ± 0.567
5.207LeuArg: 5.207 ± 0.754
4.888LeuSer: 4.888 ± 0.618
4.782LeuThr: 4.782 ± 0.555
4.676LeuVal: 4.676 ± 0.665
1.913LeuTrp: 1.913 ± 0.545
2.976LeuTyr: 2.976 ± 0.541
0.0LeuXaa: 0.0 ± 0.0
Met
3.401MetAla: 3.401 ± 0.486
0.213MetCys: 0.213 ± 0.157
2.019MetAsp: 2.019 ± 0.73
2.019MetGlu: 2.019 ± 0.543
0.85MetPhe: 0.85 ± 0.293
2.338MetGly: 2.338 ± 0.533
0.319MetHis: 0.319 ± 0.184
1.382MetIle: 1.382 ± 0.302
0.85MetLys: 0.85 ± 0.285
3.507MetLeu: 3.507 ± 0.592
0.85MetMet: 0.85 ± 0.281
1.063MetAsn: 1.063 ± 0.241
1.488MetPro: 1.488 ± 0.41
1.488MetGln: 1.488 ± 0.547
0.956MetArg: 0.956 ± 0.262
1.7MetSer: 1.7 ± 0.356
1.7MetThr: 1.7 ± 0.435
2.125MetVal: 2.125 ± 0.482
0.0MetTrp: 0.0 ± 0.0
0.638MetTyr: 0.638 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
3.719AsnAla: 3.719 ± 0.569
0.213AsnCys: 0.213 ± 0.147
3.188AsnAsp: 3.188 ± 0.785
2.657AsnGlu: 2.657 ± 0.44
1.488AsnPhe: 1.488 ± 0.44
3.932AsnGly: 3.932 ± 0.629
0.531AsnHis: 0.531 ± 0.231
3.082AsnIle: 3.082 ± 0.873
2.657AsnLys: 2.657 ± 0.449
3.188AsnLeu: 3.188 ± 0.692
0.638AsnMet: 0.638 ± 0.238
2.976AsnAsn: 2.976 ± 0.737
2.869AsnPro: 2.869 ± 0.647
2.125AsnGln: 2.125 ± 0.502
3.294AsnArg: 3.294 ± 0.852
2.869AsnSer: 2.869 ± 0.746
1.594AsnThr: 1.594 ± 0.368
2.55AsnVal: 2.55 ± 0.677
0.531AsnTrp: 0.531 ± 0.23
2.444AsnTyr: 2.444 ± 0.503
0.0AsnXaa: 0.0 ± 0.0
Pro
2.763ProAla: 2.763 ± 0.559
0.319ProCys: 0.319 ± 0.207
2.869ProAsp: 2.869 ± 0.568
3.826ProGlu: 3.826 ± 0.944
1.063ProPhe: 1.063 ± 0.324
1.807ProGly: 1.807 ± 0.367
0.638ProHis: 0.638 ± 0.225
1.169ProIle: 1.169 ± 0.483
2.763ProLys: 2.763 ± 0.604
2.232ProLeu: 2.232 ± 0.526
1.063ProMet: 1.063 ± 0.385
2.763ProAsn: 2.763 ± 0.741
1.169ProPro: 1.169 ± 0.359
1.169ProGln: 1.169 ± 0.337
2.019ProArg: 2.019 ± 0.503
2.125ProSer: 2.125 ± 0.464
1.594ProThr: 1.594 ± 0.394
2.232ProVal: 2.232 ± 0.486
0.744ProTrp: 0.744 ± 0.171
1.488ProTyr: 1.488 ± 0.45
0.0ProXaa: 0.0 ± 0.0
Gln
4.995GlnAla: 4.995 ± 1.127
0.213GlnCys: 0.213 ± 0.147
2.019GlnAsp: 2.019 ± 0.524
3.401GlnGlu: 3.401 ± 0.529
1.7GlnPhe: 1.7 ± 0.46
2.657GlnGly: 2.657 ± 0.42
0.85GlnHis: 0.85 ± 0.282
2.232GlnIle: 2.232 ± 0.414
2.55GlnLys: 2.55 ± 0.667
3.294GlnLeu: 3.294 ± 0.672
0.744GlnMet: 0.744 ± 0.267
1.807GlnAsn: 1.807 ± 0.472
1.063GlnPro: 1.063 ± 0.326
1.7GlnGln: 1.7 ± 0.382
2.232GlnArg: 2.232 ± 0.551
2.338GlnSer: 2.338 ± 0.587
2.232GlnThr: 2.232 ± 0.549
2.657GlnVal: 2.657 ± 0.519
0.638GlnTrp: 0.638 ± 0.261
1.275GlnTyr: 1.275 ± 0.456
0.0GlnXaa: 0.0 ± 0.0
Arg
6.376ArgAla: 6.376 ± 0.896
0.85ArgCys: 0.85 ± 0.29
3.613ArgAsp: 3.613 ± 0.677
4.145ArgGlu: 4.145 ± 0.807
3.082ArgPhe: 3.082 ± 0.51
4.145ArgGly: 4.145 ± 0.546
0.744ArgHis: 0.744 ± 0.229
2.657ArgIle: 2.657 ± 0.606
3.188ArgLys: 3.188 ± 0.699
5.42ArgLeu: 5.42 ± 0.748
1.382ArgMet: 1.382 ± 0.37
2.763ArgAsn: 2.763 ± 0.507
1.382ArgPro: 1.382 ± 0.368
1.913ArgGln: 1.913 ± 0.424
2.869ArgArg: 2.869 ± 0.517
5.101ArgSer: 5.101 ± 0.842
2.976ArgThr: 2.976 ± 0.365
2.976ArgVal: 2.976 ± 0.435
0.85ArgTrp: 0.85 ± 0.27
1.063ArgTyr: 1.063 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
5.42SerAla: 5.42 ± 1.231
0.531SerCys: 0.531 ± 0.228
5.632SerAsp: 5.632 ± 0.609
3.932SerGlu: 3.932 ± 0.858
2.55SerPhe: 2.55 ± 0.543
5.101SerGly: 5.101 ± 0.72
1.169SerHis: 1.169 ± 0.311
3.401SerIle: 3.401 ± 0.742
2.869SerLys: 2.869 ± 0.63
3.507SerLeu: 3.507 ± 0.737
1.169SerMet: 1.169 ± 0.399
1.7SerAsn: 1.7 ± 0.374
2.444SerPro: 2.444 ± 0.675
2.019SerGln: 2.019 ± 0.454
3.826SerArg: 3.826 ± 0.585
4.251SerSer: 4.251 ± 0.8
3.188SerThr: 3.188 ± 0.47
4.463SerVal: 4.463 ± 0.559
0.531SerTrp: 0.531 ± 0.142
2.657SerTyr: 2.657 ± 0.727
0.0SerXaa: 0.0 ± 0.0
Thr
4.782ThrAla: 4.782 ± 0.811
0.319ThrCys: 0.319 ± 0.231
2.55ThrAsp: 2.55 ± 0.537
3.294ThrGlu: 3.294 ± 0.561
1.594ThrPhe: 1.594 ± 0.436
5.845ThrGly: 5.845 ± 0.907
1.382ThrHis: 1.382 ± 0.262
3.188ThrIle: 3.188 ± 0.586
3.401ThrLys: 3.401 ± 0.44
4.57ThrLeu: 4.57 ± 0.653
1.275ThrMet: 1.275 ± 0.413
1.7ThrAsn: 1.7 ± 0.57
2.976ThrPro: 2.976 ± 0.624
1.807ThrGln: 1.807 ± 0.379
2.019ThrArg: 2.019 ± 0.356
3.826ThrSer: 3.826 ± 0.681
2.55ThrThr: 2.55 ± 0.646
4.038ThrVal: 4.038 ± 0.89
0.213ThrTrp: 0.213 ± 0.145
2.019ThrTyr: 2.019 ± 0.472
0.0ThrXaa: 0.0 ± 0.0
Val
4.57ValAla: 4.57 ± 0.714
0.425ValCys: 0.425 ± 0.249
3.719ValAsp: 3.719 ± 0.563
4.676ValGlu: 4.676 ± 0.755
1.7ValPhe: 1.7 ± 0.549
6.164ValGly: 6.164 ± 0.976
0.638ValHis: 0.638 ± 0.323
2.55ValIle: 2.55 ± 0.486
4.888ValLys: 4.888 ± 0.629
4.782ValLeu: 4.782 ± 0.789
1.594ValMet: 1.594 ± 0.457
2.763ValAsn: 2.763 ± 0.53
2.125ValPro: 2.125 ± 0.462
2.976ValGln: 2.976 ± 0.516
4.782ValArg: 4.782 ± 0.783
3.826ValSer: 3.826 ± 0.558
5.42ValThr: 5.42 ± 0.635
5.101ValVal: 5.101 ± 0.855
0.956ValTrp: 0.956 ± 0.406
1.488ValTyr: 1.488 ± 0.464
0.0ValXaa: 0.0 ± 0.0
Trp
0.956TrpAla: 0.956 ± 0.209
0.425TrpCys: 0.425 ± 0.218
0.425TrpAsp: 0.425 ± 0.204
0.956TrpGlu: 0.956 ± 0.257
0.319TrpPhe: 0.319 ± 0.184
1.169TrpGly: 1.169 ± 0.324
0.319TrpHis: 0.319 ± 0.182
0.531TrpIle: 0.531 ± 0.351
0.85TrpLys: 0.85 ± 0.333
1.275TrpLeu: 1.275 ± 0.377
0.531TrpMet: 0.531 ± 0.217
1.382TrpAsn: 1.382 ± 0.411
0.319TrpPro: 0.319 ± 0.171
0.531TrpGln: 0.531 ± 0.261
0.85TrpArg: 0.85 ± 0.274
0.638TrpSer: 0.638 ± 0.4
0.85TrpThr: 0.85 ± 0.326
1.275TrpVal: 1.275 ± 0.531
0.319TrpTrp: 0.319 ± 0.176
0.213TrpTyr: 0.213 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.976TyrAla: 2.976 ± 0.617
0.319TyrCys: 0.319 ± 0.186
2.55TyrAsp: 2.55 ± 0.788
2.019TyrGlu: 2.019 ± 0.46
0.744TyrPhe: 0.744 ± 0.36
1.913TyrGly: 1.913 ± 0.38
0.531TyrHis: 0.531 ± 0.246
2.444TyrIle: 2.444 ± 0.603
1.594TyrLys: 1.594 ± 0.309
2.657TyrLeu: 2.657 ± 0.498
1.063TyrMet: 1.063 ± 0.363
1.488TyrAsn: 1.488 ± 0.304
0.956TyrPro: 0.956 ± 0.25
1.807TyrGln: 1.807 ± 0.613
2.763TyrArg: 2.763 ± 0.546
1.382TyrSer: 1.382 ± 0.318
2.019TyrThr: 2.019 ± 0.568
2.125TyrVal: 2.125 ± 0.419
0.319TyrTrp: 0.319 ± 0.18
0.425TyrTyr: 0.425 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (9411 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski