Mink circovirus
Average proteome isoelectric point is 8.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8SQV8|W8SQV8_9CIRC Cap protein OS=Mink circovirus OX=1475143 GN=Cap PE=3 SV=1
MM1 pKa = 7.85 IMAGHH6 pKa = 6.6 PCKK9 pKa = 10.35 RR10 pKa = 11.84 YY11 pKa = 10.21 CFTINNYY18 pKa = 10.01 LPEE21 pKa = 6.0 DD22 pKa = 3.57 EE23 pKa = 5.18 AAVKK27 pKa = 10.46 EE28 pKa = 4.22 FLTEE32 pKa = 4.13 ANCVYY37 pKa = 10.74 AVVGKK42 pKa = 9.99 EE43 pKa = 3.89 VGEE46 pKa = 4.31 SGTPHH51 pKa = 6.55 LQGFCNLKK59 pKa = 10.0 KK60 pKa = 10.55 KK61 pKa = 9.69 MRR63 pKa = 11.84 FEE65 pKa = 3.98 PFKK68 pKa = 10.66 RR69 pKa = 11.84 AIGGRR74 pKa = 11.84 AHH76 pKa = 7.25 IEE78 pKa = 3.77 QSRR81 pKa = 11.84 GTDD84 pKa = 2.97 VDD86 pKa = 3.53 NKK88 pKa = 10.23 RR89 pKa = 11.84 YY90 pKa = 9.37 CSKK93 pKa = 11.18 GGDD96 pKa = 3.5 LLLEE100 pKa = 4.34 VGEE103 pKa = 4.99 PSAQGKK109 pKa = 10.21 RR110 pKa = 11.84 SDD112 pKa = 3.64 LKK114 pKa = 11.13 EE115 pKa = 3.98 AVTLLNNGGTMTDD128 pKa = 3.17 VARR131 pKa = 11.84 AHH133 pKa = 7.0 PEE135 pKa = 3.52 TFIRR139 pKa = 11.84 YY140 pKa = 8.49 GRR142 pKa = 11.84 GLRR145 pKa = 11.84 DD146 pKa = 3.4 YY147 pKa = 10.63 VIQAGLTKK155 pKa = 9.92 PRR157 pKa = 11.84 AWKK160 pKa = 8.99 TEE162 pKa = 3.42 VHH164 pKa = 6.47 VIVGPPGVGKK174 pKa = 10.24 SRR176 pKa = 11.84 HH177 pKa = 4.21 VQEE180 pKa = 4.47 TAGEE184 pKa = 3.95 NALYY188 pKa = 8.75 WKK190 pKa = 10.03 PRR192 pKa = 11.84 GKK194 pKa = 9.01 WWDD197 pKa = 3.55 GYY199 pKa = 8.86 TGQSHH204 pKa = 6.09 VVLDD208 pKa = 5.2 DD209 pKa = 4.05 FYY211 pKa = 11.9 GWLPYY216 pKa = 10.72 DD217 pKa = 4.39 DD218 pKa = 5.99 LLRR221 pKa = 11.84 LCDD224 pKa = 4.13 RR225 pKa = 11.84 YY226 pKa = 10.18 PLRR229 pKa = 11.84 VEE231 pKa = 4.35 TKK233 pKa = 8.08 GGTVEE238 pKa = 5.39 FVAKK242 pKa = 9.8 VIWITSNKK250 pKa = 7.52 QVKK253 pKa = 9.23 DD254 pKa = 3.0 WYY256 pKa = 10.23 DD257 pKa = 3.67 YY258 pKa = 11.91 EE259 pKa = 4.14 EE260 pKa = 4.86 LKK262 pKa = 10.89 VDD264 pKa = 3.46 ARR266 pKa = 11.84 ALYY269 pKa = 10.23 RR270 pKa = 11.84 RR271 pKa = 11.84 LTTYY275 pKa = 10.56 QVMRR279 pKa = 11.84 QGGEE283 pKa = 3.99 LYY285 pKa = 10.04 NVQMTGDD292 pKa = 3.53 NKK294 pKa = 10.49 INYY297 pKa = 8.4
Molecular weight: 33.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.881
IPC2_protein 8.053
IPC_protein 7.966
Toseland 7.966
ProMoST 8.551
Dawson 8.697
Bjellqvist 8.843
Wikipedia 8.712
Rodwell 8.741
Grimsley 7.922
Solomon 8.814
Lehninger 8.799
Nozaki 8.931
DTASelect 8.653
Thurlkill 8.712
EMBOSS 8.858
Sillero 8.975
Patrickios 4.291
IPC_peptide 8.799
IPC2_peptide 7.673
IPC2.peptide.svr19 7.848
Protein with the highest isoelectric point:
>tr|W8SQV8|W8SQV8_9CIRC Cap protein OS=Mink circovirus OX=1475143 GN=Cap PE=3 SV=1
MM1 pKa = 7.73 PVRR4 pKa = 11.84 SRR6 pKa = 11.84 YY7 pKa = 7.8 SRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 NRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 GPQRR24 pKa = 11.84 RR25 pKa = 11.84 YY26 pKa = 10.46 ARR28 pKa = 11.84 GGYY31 pKa = 8.14 RR32 pKa = 11.84 WRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 10.0 NGIFNARR43 pKa = 11.84 LQTEE47 pKa = 4.15 EE48 pKa = 4.3 TLTVASAATPSWCVGHH64 pKa = 6.73 VNVTLKK70 pKa = 11.3 SMMPKK75 pKa = 10.66 GPGTTTNLYY84 pKa = 10.72 GMPFSYY90 pKa = 10.84 YY91 pKa = 10.36 RR92 pKa = 11.84 IRR94 pKa = 11.84 KK95 pKa = 8.94 LKK97 pKa = 10.45 IEE99 pKa = 4.52 FLPQIGITSNRR110 pKa = 11.84 SYY112 pKa = 11.36 GGTAIILDD120 pKa = 3.65 GDD122 pKa = 4.31 YY123 pKa = 10.79 ISEE126 pKa = 4.45 GKK128 pKa = 8.66 NQTYY132 pKa = 10.76 DD133 pKa = 3.31 PLANHH138 pKa = 6.51 SSRR141 pKa = 11.84 HH142 pKa = 4.93 NLTNIHH148 pKa = 5.45 RR149 pKa = 11.84 HH150 pKa = 4.68 SRR152 pKa = 11.84 YY153 pKa = 7.57 FTPKK157 pKa = 8.81 PQHH160 pKa = 5.74 SSKK163 pKa = 11.04 NISTYY168 pKa = 9.15 FQPNNKK174 pKa = 8.63 QLQEE178 pKa = 4.39 WISMNQQDD186 pKa = 4.21 RR187 pKa = 11.84 VHH189 pKa = 6.92 HH190 pKa = 5.97 GLQYY194 pKa = 10.78 SIQNSNFIQTWTIRR208 pKa = 11.84 KK209 pKa = 5.44 TAYY212 pKa = 9.0 VQFRR216 pKa = 11.84 EE217 pKa = 3.82 FDD219 pKa = 3.58 LVNFPKK225 pKa = 9.35 QTT227 pKa = 3.27
Molecular weight: 26.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 10.292
IPC_protein 11.257
Toseland 11.213
ProMoST 11.491
Dawson 11.272
Bjellqvist 11.169
Wikipedia 11.652
Rodwell 11.242
Grimsley 11.33
Solomon 11.608
Lehninger 11.535
Nozaki 11.199
DTASelect 11.155
Thurlkill 11.213
EMBOSS 11.667
Sillero 11.242
Patrickios 10.935
IPC_peptide 11.608
IPC2_peptide 10.511
IPC2.peptide.svr19 8.464
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
524
227
297
262.0
30.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.962 ± 1.0
1.336 ± 0.623
4.198 ± 1.389
4.962 ± 1.613
3.244 ± 0.501
8.397 ± 1.551
2.863 ± 0.46
4.58 ± 1.104
6.298 ± 1.01
6.679 ± 0.969
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.099 ± 0.072
5.534 ± 1.36
4.58 ± 0.491
4.771 ± 1.278
9.351 ± 2.076
4.962 ± 2.065
6.87 ± 0.737
6.298 ± 1.93
2.29 ± 0.061
5.725 ± 0.001
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here