Avian metaavulavirus 5
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3X605|D3X605_9MONO Isoform of D3X603 W protein OS=Avian metaavulavirus 5 OX=2560315 GN=P PE=4 SV=1
MM1 pKa = 8.41 DD2 pKa = 3.94 ITDD5 pKa = 4.13 DD6 pKa = 4.33 QAILDD11 pKa = 4.9 LLTLSNDD18 pKa = 3.38 VIEE21 pKa = 5.2 SIQHH25 pKa = 5.86 AEE27 pKa = 4.29 SAGSQPPTYY36 pKa = 10.47 GRR38 pKa = 11.84 STIPKK43 pKa = 7.35 GTTMALTQAWEE54 pKa = 4.24 AEE56 pKa = 4.5 SNPTQPQPSISPSQLDD72 pKa = 4.19 HH73 pKa = 7.13 IRR75 pKa = 11.84 VDD77 pKa = 3.25 QDD79 pKa = 3.6 SNKK82 pKa = 9.89 NHH84 pKa = 7.36 DD85 pKa = 3.78 NLKK88 pKa = 9.73 NQFDD92 pKa = 4.3 SHH94 pKa = 6.21 HH95 pKa = 6.46 HH96 pKa = 5.82 TSQDD100 pKa = 3.6 SEE102 pKa = 4.47 THH104 pKa = 6.36 PNIQVNPSVNRR115 pKa = 11.84 TNEE118 pKa = 3.69 EE119 pKa = 4.21 DD120 pKa = 3.38 NSKK123 pKa = 10.42 LVQQRR128 pKa = 11.84 DD129 pKa = 3.4 PSTTWEE135 pKa = 4.53 GGSMLDD141 pKa = 4.4 RR142 pKa = 11.84 EE143 pKa = 4.4 LDD145 pKa = 4.25 AIQSKK150 pKa = 10.03 IKK152 pKa = 10.24 RR153 pKa = 11.84 GKK155 pKa = 4.42 TTKK158 pKa = 10.11 SAHH161 pKa = 5.58 QPSHH165 pKa = 5.93 TATHH169 pKa = 5.9 QSPNNLDD176 pKa = 4.28 DD177 pKa = 5.61 DD178 pKa = 4.3 IKK180 pKa = 11.25 KK181 pKa = 10.61 GGPDD185 pKa = 3.22 LRR187 pKa = 6.06
Molecular weight: 20.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.182
IPC2_protein 5.054
IPC_protein 5.029
Toseland 5.156
ProMoST 5.27
Dawson 5.13
Bjellqvist 5.219
Wikipedia 5.08
Rodwell 5.054
Grimsley 5.143
Solomon 5.13
Lehninger 5.105
Nozaki 5.308
DTASelect 5.525
Thurlkill 5.207
EMBOSS 5.232
Sillero 5.372
Patrickios 4.355
IPC_peptide 5.143
IPC2_peptide 5.372
IPC2.peptide.svr19 5.302
Protein with the highest isoelectric point:
>tr|D3X607|D3X607_9MONO Fusion glycoprotein F0 OS=Avian metaavulavirus 5 OX=2560315 GN=F PE=3 SV=1
MM1 pKa = 7.5 AQTQLKK7 pKa = 10.42 LYY9 pKa = 10.56 VNDD12 pKa = 4.07 GDD14 pKa = 3.83 PSNRR18 pKa = 11.84 LLAFPIVMKK27 pKa = 10.67 EE28 pKa = 3.8 SAQGGKK34 pKa = 9.32 VLQPQLRR41 pKa = 11.84 ISYY44 pKa = 10.09 LGDD47 pKa = 4.42 AIGGRR52 pKa = 11.84 NSVIFINCYY61 pKa = 10.47 GFIEE65 pKa = 4.45 SMKK68 pKa = 10.51 AGEE71 pKa = 4.93 GPFLDD76 pKa = 4.09 VNSDD80 pKa = 3.35 GKK82 pKa = 11.53 GEE84 pKa = 4.29 VITAAGLTLGSVTYY98 pKa = 10.66 DD99 pKa = 3.2 SDD101 pKa = 3.41 PTEE104 pKa = 4.21 IARR107 pKa = 11.84 SCYY110 pKa = 9.83 QLLVTVKK117 pKa = 9.94 KK118 pKa = 10.79 SADD121 pKa = 3.16 NTEE124 pKa = 3.97 RR125 pKa = 11.84 VVYY128 pKa = 8.99 TLASKK133 pKa = 10.6 PPALSSSRR141 pKa = 11.84 VVTSGGCILSAEE153 pKa = 4.41 EE154 pKa = 4.56 AVKK157 pKa = 10.72 CPSKK161 pKa = 10.42 LQSGIPYY168 pKa = 9.25 KK169 pKa = 10.46 FRR171 pKa = 11.84 IMFVSLTYY179 pKa = 10.14 IHH181 pKa = 6.69 QSTLYY186 pKa = 10.21 RR187 pKa = 11.84 VNNLIAKK194 pKa = 8.94 LRR196 pKa = 11.84 SPVFISVQLQVTLILDD212 pKa = 4.13 LPEE215 pKa = 3.91 KK216 pKa = 10.65 HH217 pKa = 6.83 PMGKK221 pKa = 9.87 YY222 pKa = 10.55 LINQDD227 pKa = 3.45 GVYY230 pKa = 9.88 KK231 pKa = 10.47 AHH233 pKa = 6.61 IWMHH237 pKa = 5.35 ICNFKK242 pKa = 9.0 KK243 pKa = 9.32 TNRR246 pKa = 11.84 KK247 pKa = 8.76 GADD250 pKa = 2.98 RR251 pKa = 11.84 SVLQIKK257 pKa = 9.92 EE258 pKa = 4.31 KK259 pKa = 8.93 VRR261 pKa = 11.84 KK262 pKa = 8.71 MGLKK266 pKa = 8.67 VTLADD271 pKa = 3.26 LWGPTVVVEE280 pKa = 4.44 ATGTMSKK287 pKa = 10.33 YY288 pKa = 10.41 AVGFFSEE295 pKa = 4.88 TKK297 pKa = 10.18 VSCHH301 pKa = 6.76 PISKK305 pKa = 9.91 ISPEE309 pKa = 3.8 VAKK312 pKa = 10.5 IIWACTTTIGQAVVIIQASSRR333 pKa = 11.84 SEE335 pKa = 3.92 LLTAEE340 pKa = 5.27 DD341 pKa = 4.56 IEE343 pKa = 5.03 CKK345 pKa = 9.86 GTTSIKK351 pKa = 10.21 KK352 pKa = 9.33 SAVKK356 pKa = 10.21 EE357 pKa = 3.82 FSLFSKK363 pKa = 9.75 PAKK366 pKa = 10.18
Molecular weight: 40.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.793
IPC2_protein 8.668
IPC_protein 8.536
Toseland 9.311
ProMoST 9.151
Dawson 9.589
Bjellqvist 9.37
Wikipedia 9.736
Rodwell 9.926
Grimsley 9.633
Solomon 9.633
Lehninger 9.589
Nozaki 9.56
DTASelect 9.282
Thurlkill 9.458
EMBOSS 9.765
Sillero 9.589
Patrickios 4.914
IPC_peptide 9.619
IPC2_peptide 8.024
IPC2.peptide.svr19 7.838
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5122
187
2263
640.3
71.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.111 ± 0.723
2.148 ± 0.337
4.979 ± 0.695
4.666 ± 0.249
3.182 ± 0.498
5.037 ± 0.361
2.382 ± 0.393
7.927 ± 0.598
4.861 ± 0.534
10.406 ± 0.996
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.245 ± 0.288
5.174 ± 0.637
4.861 ± 0.539
5.271 ± 0.575
4.627 ± 0.319
9.762 ± 0.428
7.321 ± 0.432
5.018 ± 0.672
1.132 ± 0.235
2.889 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here