Cellulomonas marina
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3460 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I0VWE3|A0A1I0VWE3_9CELL Deoxyguanosinetriphosphate triphosphohydrolase-like protein OS=Cellulomonas marina OX=988821 GN=SAMN05421867_1026 PE=3 SV=1
MM1 pKa = 7.54 RR2 pKa = 11.84 RR3 pKa = 11.84 ITSTVAMMAGVTLLAAACSGGGGSTSAEE31 pKa = 4.01 EE32 pKa = 4.53 SPSSSAAAEE41 pKa = 4.07 PSASEE46 pKa = 3.7 SAPPVRR52 pKa = 11.84 GDD54 pKa = 3.45 EE55 pKa = 4.27 EE56 pKa = 4.42 LVIWTDD62 pKa = 3.26 ALKK65 pKa = 10.74 LDD67 pKa = 4.01 AVRR70 pKa = 11.84 AVADD74 pKa = 4.0 QFAEE78 pKa = 4.4 DD79 pKa = 3.34 NGITVGVQAIVNTRR93 pKa = 11.84 TDD95 pKa = 4.61 FITANAAGNGPDD107 pKa = 3.73 VVVGAHH113 pKa = 6.32 DD114 pKa = 4.31 WIGQLVQNGAIDD126 pKa = 4.17 PLQLSAADD134 pKa = 3.58 LGGYY138 pKa = 9.69 AEE140 pKa = 4.35 NAIQAVTYY148 pKa = 10.18 DD149 pKa = 3.52 GQIYY153 pKa = 8.83 GLPYY157 pKa = 10.14 GVEE160 pKa = 4.19 ALGLYY165 pKa = 9.65 CNKK168 pKa = 10.25 AYY170 pKa = 10.42 APDD173 pKa = 4.16 TYY175 pKa = 11.55 ASLDD179 pKa = 3.66 DD180 pKa = 4.73 VIAAGQAAVDD190 pKa = 3.93 AGQVEE195 pKa = 4.59 TSLNVAQGDD204 pKa = 3.87 VGDD207 pKa = 4.78 AYY209 pKa = 11.03 HH210 pKa = 6.15 MQPILTSMGGYY221 pKa = 10.64 LFGQNPDD228 pKa = 2.88 GTYY231 pKa = 10.68 NPEE234 pKa = 4.24 DD235 pKa = 4.26 LGLASPGGLAAAQKK249 pKa = 10.37 IFDD252 pKa = 4.55 LGEE255 pKa = 3.83 QGSNVLRR262 pKa = 11.84 RR263 pKa = 11.84 SISGDD268 pKa = 2.89 NSIALFAEE276 pKa = 4.75 GRR278 pKa = 11.84 AACLISGPWALADD291 pKa = 3.48 VRR293 pKa = 11.84 EE294 pKa = 4.25 GLGEE298 pKa = 4.59 DD299 pKa = 5.04 GYY301 pKa = 9.33 TLQPIPGFAGQQPAQPFLGAQAFYY325 pKa = 11.1 VAANGQNKK333 pKa = 7.95 TFAQEE338 pKa = 3.93 FVTNGVNNAEE348 pKa = 4.49 GMTTMYY354 pKa = 10.51 EE355 pKa = 4.15 LANLPPALTEE365 pKa = 4.0 VRR367 pKa = 11.84 QSIAADD373 pKa = 3.42 NPDD376 pKa = 3.61 FEE378 pKa = 5.38 VFAQAADD385 pKa = 3.65 NGNPMPAVPAMAEE398 pKa = 3.68 VWAPLGQAYY407 pKa = 10.14 SAIIGGADD415 pKa = 3.58 PASTMQQAQDD425 pKa = 3.79 TIAAAIAASS434 pKa = 3.48
Molecular weight: 44.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 0.922
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1I0XJB0|A0A1I0XJB0_9CELL 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Cellulomonas marina OX=988821 GN=menD PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3460
0
3460
1193049
30
2100
344.8
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.438 ± 0.07
0.535 ± 0.011
6.375 ± 0.031
5.198 ± 0.039
2.227 ± 0.025
9.9 ± 0.045
2.095 ± 0.019
2.104 ± 0.029
1.113 ± 0.021
10.608 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.355 ± 0.017
1.224 ± 0.018
6.364 ± 0.042
2.501 ± 0.023
8.361 ± 0.043
4.462 ± 0.03
6.347 ± 0.042
10.65 ± 0.047
1.505 ± 0.018
1.638 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here