Legionella septentrionalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0V9U0|A0A3S0V9U0_9GAMM Uncharacterized protein OS=Legionella septentrionalis OX=2498109 GN=EKM59_08970 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.37RR3 pKa = 11.84ILLLSFFTCFSLTVNAADD21 pKa = 4.88DD22 pKa = 4.27PPNIADD28 pKa = 5.3AEE30 pKa = 4.13NLDD33 pKa = 4.7AKK35 pKa = 10.36MCVDD39 pKa = 3.77NTANDD44 pKa = 4.07CVNTVCLNSEE54 pKa = 3.9EE55 pKa = 4.3RR56 pKa = 11.84DD57 pKa = 4.21CIEE60 pKa = 3.8QCQADD65 pKa = 4.0AQAKK69 pKa = 10.19CNVQADD75 pKa = 4.07EE76 pKa = 4.26

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433JMH5|A0A433JMH5_9GAMM Chaperone protein DnaK OS=Legionella septentrionalis OX=2498109 GN=dnaK PE=2 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84DD15 pKa = 3.36HH16 pKa = 6.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84TGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2276

0

2276

750551

28

3998

329.8

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.741 ± 0.052

1.211 ± 0.02

4.581 ± 0.035

6.175 ± 0.049

4.46 ± 0.036

6.112 ± 0.048

2.616 ± 0.029

7.059 ± 0.048

6.018 ± 0.054

11.171 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.023

4.464 ± 0.045

4.203 ± 0.031

4.811 ± 0.04

4.591 ± 0.039

5.887 ± 0.037

5.07 ± 0.045

5.98 ± 0.047

1.152 ± 0.021

3.321 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski