Boa constrictor papillomavirus 1
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346CL00|A0A346CL00_9PAPI Protein E6 OS=Boa constrictor papillomavirus 1 OX=2294156 GN=E6 PE=3 SV=1
MM1 pKa = 7.69 IGPKK5 pKa = 8.56 PTLLDD10 pKa = 3.18 IDD12 pKa = 4.64 LFCHH16 pKa = 5.72 EE17 pKa = 4.8 TLQLSDD23 pKa = 4.64 EE24 pKa = 4.33 EE25 pKa = 4.48 DD26 pKa = 3.77 TPVVQPAVDD35 pKa = 3.48 QLQQPFEE42 pKa = 4.18 IQLGCAYY49 pKa = 10.23 CSVGLRR55 pKa = 11.84 LVVAASPSAIRR66 pKa = 11.84 ILNRR70 pKa = 11.84 LLLLDD75 pKa = 5.76 FGFLCNDD82 pKa = 3.95 CSDD85 pKa = 4.75 SIQDD89 pKa = 3.74 GSNHH93 pKa = 6.23 GGG95 pKa = 3.18
Molecular weight: 10.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 0.846
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|A0A346CL02|A0A346CL02_9PAPI Minor capsid protein L2 OS=Boa constrictor papillomavirus 1 OX=2294156 GN=L2 PE=3 SV=1
MM1 pKa = 7.29 TMKK4 pKa = 10.33 CGKK7 pKa = 9.32 RR8 pKa = 11.84 QLGRR12 pKa = 11.84 LIIMAHH18 pKa = 6.2 ILYY21 pKa = 9.91 EE22 pKa = 4.05 VALNRR27 pKa = 11.84 IILILRR33 pKa = 11.84 LKK35 pKa = 8.76 PRR37 pKa = 11.84 RR38 pKa = 11.84 WVYY41 pKa = 11.24 SHH43 pKa = 6.36 GQLNIHH49 pKa = 6.73 ISYY52 pKa = 10.32 FLEE55 pKa = 4.22 NQPRR59 pKa = 11.84 PVAPVSLKK67 pKa = 10.41 EE68 pKa = 3.79 GQLRR72 pKa = 11.84 FRR74 pKa = 11.84 LLDD77 pKa = 3.97 EE78 pKa = 4.77 DD79 pKa = 4.73 TEE81 pKa = 4.82 DD82 pKa = 5.89 DD83 pKa = 3.76 PTAQDD88 pKa = 2.95 KK89 pKa = 10.62 RR90 pKa = 11.84 RR91 pKa = 11.84 QRR93 pKa = 11.84 EE94 pKa = 3.78 QKK96 pKa = 10.56 KK97 pKa = 10.52 EE98 pKa = 3.89 DD99 pKa = 3.71 FQSLLRR105 pKa = 11.84 TLEE108 pKa = 4.09 RR109 pKa = 11.84 LTQRR113 pKa = 11.84 LRR115 pKa = 11.84 QNIEE119 pKa = 3.65 TALNDD124 pKa = 3.63 YY125 pKa = 10.49 ALKK128 pKa = 10.15 QQIPQFF134 pKa = 3.84
Molecular weight: 16.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.196
IPC2_protein 9.311
IPC_protein 9.531
Toseland 10.16
ProMoST 9.809
Dawson 10.321
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.628
Grimsley 10.379
Solomon 10.379
Lehninger 10.35
Nozaki 10.145
DTASelect 9.984
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.233
Patrickios 10.335
IPC_peptide 10.379
IPC2_peptide 8.653
IPC2.peptide.svr19 8.412
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2314
95
617
330.6
37.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.748 ± 0.368
2.334 ± 1.056
5.618 ± 0.691
7.087 ± 0.493
4.062 ± 0.399
6.007 ± 1.058
1.772 ± 0.177
6.569 ± 0.429
5.143 ± 0.86
8.384 ± 1.103
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.685 ± 0.321
4.322 ± 0.29
5.704 ± 0.933
4.149 ± 0.7
6.396 ± 0.782
7.044 ± 0.642
6.742 ± 0.867
6.525 ± 0.825
1.08 ± 0.272
3.63 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here