Enterobacteria phage phi92

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7HXE7|I7HXE7_9CAUD Phi92_gp104 OS=Enterobacteria phage phi92 OX=948870 GN=PHI92_gene_104 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 5.62IYY4 pKa = 10.51SGEE7 pKa = 3.98GKK9 pKa = 10.71YY10 pKa = 8.23FTVVDD15 pKa = 3.53NGEE18 pKa = 4.17IIIYY22 pKa = 9.93RR23 pKa = 11.84YY24 pKa = 9.49GSEE27 pKa = 3.64WRR29 pKa = 11.84NEE31 pKa = 4.02TGDD34 pKa = 4.02GYY36 pKa = 10.6ILSLLRR42 pKa = 11.84LINDD46 pKa = 3.33LTEE49 pKa = 4.09EE50 pKa = 4.19LEE52 pKa = 4.27EE53 pKa = 4.31TKK55 pKa = 10.39RR56 pKa = 11.84SYY58 pKa = 11.51DD59 pKa = 3.61DD60 pKa = 3.87QVDD63 pKa = 3.69SLEE66 pKa = 4.92NIISDD71 pKa = 3.96LNEE74 pKa = 3.93EE75 pKa = 4.07VCYY78 pKa = 10.86LSGQVSEE85 pKa = 4.74LTLEE89 pKa = 4.48CEE91 pKa = 4.48DD92 pKa = 4.7LGDD95 pKa = 4.76VIADD99 pKa = 3.52LNEE102 pKa = 4.0RR103 pKa = 11.84LKK105 pKa = 11.13DD106 pKa = 3.47

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7HXB3|I7HXB3_9CAUD Phi92_gp029 OS=Enterobacteria phage phi92 OX=948870 GN=PHI92_gene_029 PE=4 SV=1
MM1 pKa = 6.67VRR3 pKa = 11.84NRR5 pKa = 11.84GLHH8 pKa = 5.2KK9 pKa = 10.21RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 4.29LTYY15 pKa = 8.46WHH17 pKa = 6.81RR18 pKa = 11.84KK19 pKa = 4.74VHH21 pKa = 5.92EE22 pKa = 4.29PVSKK26 pKa = 10.99KK27 pKa = 7.65EE28 pKa = 4.03WQYY31 pKa = 11.63LPYY34 pKa = 10.39GKK36 pKa = 9.78EE37 pKa = 3.43RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 10.47YY41 pKa = 9.81LLAHH45 pKa = 6.29LRR47 pKa = 11.84VHH49 pKa = 6.99GSLPVNSKK57 pKa = 8.75WQLQTAYY64 pKa = 10.46DD65 pKa = 3.97EE66 pKa = 4.32DD67 pKa = 3.64MKK69 pKa = 11.51YY70 pKa = 10.73LLKK73 pKa = 10.69RR74 pKa = 11.84GIICLVKK81 pKa = 9.74IGRR84 pKa = 11.84WKK86 pKa = 10.45SRR88 pKa = 11.84QSYY91 pKa = 9.95IVLKK95 pKa = 9.17QEE97 pKa = 3.92KK98 pKa = 9.55RR99 pKa = 3.43

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

250

0

250

45527

34

1104

182.1

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.508 ± 0.143

1.419 ± 0.085

6.532 ± 0.118

7.292 ± 0.213

4.509 ± 0.115

6.42 ± 0.177

1.887 ± 0.085

6.407 ± 0.114

7.051 ± 0.204

8.103 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.094

5.28 ± 0.136

3.38 ± 0.122

3.255 ± 0.114

3.976 ± 0.114

5.948 ± 0.126

6.155 ± 0.205

7.092 ± 0.172

1.535 ± 0.104

4.624 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski