Cochleicola gelatinilyticus
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A167G804|A0A167G804_9FLAO tRNA modification GTPase MnmE OS=Cochleicola gelatinilyticus OX=1763537 GN=mnmE PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.4 KK3 pKa = 10.27 LLYY6 pKa = 10.56 LFVSGLILASCSVDD20 pKa = 3.31 QPEE23 pKa = 4.39 TSEE26 pKa = 4.42 SNADD30 pKa = 3.64 LAYY33 pKa = 10.49 DD34 pKa = 4.46 ASTLDD39 pKa = 3.63 ASSLGNYY46 pKa = 9.35 KK47 pKa = 10.64 GVFTTNNSDD56 pKa = 3.05 YY57 pKa = 10.46 RR58 pKa = 11.84 ATVEE62 pKa = 3.88 IDD64 pKa = 3.24 VPYY67 pKa = 10.39 FNSEE71 pKa = 3.65 NTTKK75 pKa = 10.52 QKK77 pKa = 10.73 SYY79 pKa = 8.8 PTALVSLQNGKK90 pKa = 10.09 SFVATANRR98 pKa = 11.84 NISLGEE104 pKa = 4.29 EE105 pKa = 3.7 ITKK108 pKa = 10.73 LEE110 pKa = 4.12 FSGSEE115 pKa = 3.69 MSFKK119 pKa = 10.77 FSVNDD124 pKa = 4.34 DD125 pKa = 3.64 GTSPIITDD133 pKa = 3.33 VNYY136 pKa = 10.85 KK137 pKa = 9.35 NLKK140 pKa = 10.01 GDD142 pKa = 3.42 ILVAKK147 pKa = 7.87 NTSFAPVTTFTGTYY161 pKa = 9.93 QCFQCGNSEE170 pKa = 3.85 PRR172 pKa = 11.84 TWNVVISGDD181 pKa = 3.37 ATGDD185 pKa = 3.24 QTYY188 pKa = 8.26 ATQVEE193 pKa = 5.03 FNGNTYY199 pKa = 10.78 SGGQGLQEE207 pKa = 4.01 NCVVDD212 pKa = 4.01 GTNSDD217 pKa = 3.18 ITFCTSRR224 pKa = 11.84 SGDD227 pKa = 3.18 AGSNVGFMVGSNAVEE242 pKa = 4.15 WEE244 pKa = 4.01 GQMGYY249 pKa = 10.84 SNSAPDD255 pKa = 4.0 CSSQVGFWYY264 pKa = 9.82 FKK266 pKa = 10.68 RR267 pKa = 11.84 GLEE270 pKa = 4.18 GQKK273 pKa = 10.51 SGTFKK278 pKa = 10.45 TDD280 pKa = 2.43 AMNPEE285 pKa = 4.23 SPNCFTRR292 pKa = 11.84 LAFEE296 pKa = 4.25 NFEE299 pKa = 5.46 DD300 pKa = 3.92 DD301 pKa = 3.27 TLMYY305 pKa = 7.42 TTSEE309 pKa = 4.34 PEE311 pKa = 4.04 FTSGNDD317 pKa = 3.37 DD318 pKa = 3.58 YY319 pKa = 11.11 FTRR322 pKa = 11.84 TDD324 pKa = 3.25 GSNIGASFNNFSNLIGNGYY343 pKa = 9.23 FAAQDD348 pKa = 3.25 VDD350 pKa = 4.06 SQIGIPAYY358 pKa = 10.02 IMFEE362 pKa = 4.3 NINIASFSTVYY373 pKa = 10.68 VDD375 pKa = 5.22 ASFAEE380 pKa = 4.87 DD381 pKa = 3.5 DD382 pKa = 4.26 ASNNDD387 pKa = 3.78 EE388 pKa = 5.32 DD389 pKa = 4.84 WDD391 pKa = 3.93 NTDD394 pKa = 3.33 NVIMEE399 pKa = 4.01 YY400 pKa = 10.81 SFDD403 pKa = 3.78 GTNWTPILAITNDD416 pKa = 3.17 GSQFNSAPQIDD427 pKa = 3.95 TDD429 pKa = 3.97 FDD431 pKa = 4.22 GVGDD435 pKa = 3.85 GVIITEE441 pKa = 4.53 TFQDD445 pKa = 3.47 FRR447 pKa = 11.84 VAFNNNVANNPTASGTVAIRR467 pKa = 11.84 IAINLNAGDD476 pKa = 4.12 EE477 pKa = 4.88 DD478 pKa = 3.85 IAIDD482 pKa = 3.89 NILIRR487 pKa = 11.84 GYY489 pKa = 11.05
Molecular weight: 53.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.935
Patrickios 1.354
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A167H472|A0A167H472_9FLAO tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase MtaB OS=Cochleicola gelatinilyticus OX=1763537 GN=ULVI_12020 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.18 RR22 pKa = 11.84 MASVNGRR29 pKa = 11.84 KK30 pKa = 9.17 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.87 GRR40 pKa = 11.84 KK41 pKa = 8.56 KK42 pKa = 10.21 ISVSSEE48 pKa = 3.74 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2962
0
2962
1004104
50
3207
339.0
38.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.678 ± 0.033
0.714 ± 0.016
5.514 ± 0.034
6.986 ± 0.044
5.202 ± 0.037
6.527 ± 0.059
1.756 ± 0.022
7.589 ± 0.039
7.268 ± 0.069
9.208 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.154 ± 0.022
5.895 ± 0.046
3.47 ± 0.03
3.418 ± 0.028
3.594 ± 0.031
6.492 ± 0.033
6.309 ± 0.05
6.314 ± 0.035
0.977 ± 0.016
3.937 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here