Amino acid dipepetide frequency for Streptomyces sp. 70

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.374AlaAla: 20.374 ± 0.108	1.103AlaCys: 1.103 ± 0.021	8.118AlaAsp: 8.118 ± 0.066	8.52AlaGlu: 8.52 ± 0.067	3.522AlaPhe: 3.522 ± 0.04	12.512AlaGly: 12.512 ± 0.093	2.915AlaHis: 2.915 ± 0.036	3.555AlaIle: 3.555 ± 0.041	2.922AlaLys: 2.922 ± 0.04	14.274AlaLeu: 14.274 ± 0.092	2.415AlaMet: 2.415 ± 0.029	1.979AlaAsn: 1.979 ± 0.029	6.785AlaPro: 6.785 ± 0.071	3.945AlaGln: 3.945 ± 0.039	9.843AlaArg: 9.843 ± 0.072	6.077AlaSer: 6.077 ± 0.046	7.028AlaThr: 7.028 ± 0.054	12.17AlaVal: 12.17 ± 0.079	1.887AlaTrp: 1.887 ± 0.026	2.801AlaTyr: 2.801 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.068CysAla: 1.068 ± 0.022	0.103CysCys: 0.103 ± 0.006	0.478CysAsp: 0.478 ± 0.014	0.419CysGlu: 0.419 ± 0.013	0.21CysPhe: 0.21 ± 0.009	0.966CysGly: 0.966 ± 0.02	0.203CysHis: 0.203 ± 0.009	0.193CysIle: 0.193 ± 0.007	0.149CysLys: 0.149 ± 0.007	0.793CysLeu: 0.793 ± 0.017	0.132CysMet: 0.132 ± 0.006	0.154CysAsn: 0.154 ± 0.008	0.49CysPro: 0.49 ± 0.014	0.175CysGln: 0.175 ± 0.008	0.633CysArg: 0.633 ± 0.015	0.478CysSer: 0.478 ± 0.013	0.573CysThr: 0.573 ± 0.015	0.661CysVal: 0.661 ± 0.015	0.13CysTrp: 0.13 ± 0.007	0.172CysTyr: 0.172 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.295AspAla: 7.295 ± 0.057	0.442AspCys: 0.442 ± 0.014	3.437AspAsp: 3.437 ± 0.033	3.802AspGlu: 3.802 ± 0.041	1.696AspPhe: 1.696 ± 0.025	6.303AspGly: 6.303 ± 0.051	1.458AspHis: 1.458 ± 0.02	1.903AspIle: 1.903 ± 0.027	1.26AspLys: 1.26 ± 0.023	6.162AspLeu: 6.162 ± 0.045	0.833AspMet: 0.833 ± 0.02	1.052AspAsn: 1.052 ± 0.018	4.359AspPro: 4.359 ± 0.039	1.589AspGln: 1.589 ± 0.027	4.702AspArg: 4.702 ± 0.044	2.645AspSer: 2.645 ± 0.033	3.271AspThr: 3.271 ± 0.034	4.727AspVal: 4.727 ± 0.039	1.062AspTrp: 1.062 ± 0.018	1.172AspTyr: 1.172 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.255GluAla: 7.255 ± 0.069	0.385GluCys: 0.385 ± 0.011	2.798GluAsp: 2.798 ± 0.03	3.516GluGlu: 3.516 ± 0.043	1.481GluPhe: 1.481 ± 0.021	4.22GluGly: 4.22 ± 0.036	1.522GluHis: 1.522 ± 0.023	2.209GluIle: 2.209 ± 0.031	1.474GluLys: 1.474 ± 0.028	6.745GluLeu: 6.745 ± 0.063	0.864GluMet: 0.864 ± 0.016	1.045GluAsn: 1.045 ± 0.02	3.316GluPro: 3.316 ± 0.039	2.363GluGln: 2.363 ± 0.032	5.186GluArg: 5.186 ± 0.046	2.542GluSer: 2.542 ± 0.034	2.862GluThr: 2.862 ± 0.031	4.382GluVal: 4.382 ± 0.042	0.765GluTrp: 0.765 ± 0.017	1.136GluTyr: 1.136 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.614PheAla: 3.614 ± 0.041	0.273PheCys: 0.273 ± 0.01	1.982PheAsp: 1.982 ± 0.026	1.454PheGlu: 1.454 ± 0.023	0.907PhePhe: 0.907 ± 0.019	3.055PheGly: 3.055 ± 0.032	0.651PheHis: 0.651 ± 0.015	0.723PheIle: 0.723 ± 0.017	0.576PheLys: 0.576 ± 0.015	2.61PheLeu: 2.61 ± 0.034	0.401PheMet: 0.401 ± 0.013	0.588PheAsn: 0.588 ± 0.014	1.399PhePro: 1.399 ± 0.02	0.719PheGln: 0.719 ± 0.016	1.842PheArg: 1.842 ± 0.026	1.549PheSer: 1.549 ± 0.024	2.03PheThr: 2.03 ± 0.03	2.249PheVal: 2.249 ± 0.029	0.438PheTrp: 0.438 ± 0.012	0.597PheTyr: 0.597 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	10.616GlyAla: 10.616 ± 0.076	0.848GlyCys: 0.848 ± 0.019	4.949GlyAsp: 4.949 ± 0.044	4.931GlyGlu: 4.931 ± 0.044	2.874GlyPhe: 2.874 ± 0.037	8.583GlyGly: 8.583 ± 0.077	2.431GlyHis: 2.431 ± 0.034	3.581GlyIle: 3.581 ± 0.04	2.489GlyLys: 2.489 ± 0.036	9.423GlyLeu: 9.423 ± 0.069	1.976GlyMet: 1.976 ± 0.028	1.922GlyAsn: 1.922 ± 0.031	4.976GlyPro: 4.976 ± 0.045	2.733GlyGln: 2.733 ± 0.034	7.569GlyArg: 7.569 ± 0.053	5.673GlySer: 5.673 ± 0.049	6.466GlyThr: 6.466 ± 0.063	7.32GlyVal: 7.32 ± 0.057	1.725GlyTrp: 1.725 ± 0.026	2.282GlyTyr: 2.282 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.702HisAla: 2.702 ± 0.028	0.216HisCys: 0.216 ± 0.01	1.378HisAsp: 1.378 ± 0.023	1.256HisGlu: 1.256 ± 0.021	0.684HisPhe: 0.684 ± 0.016	2.496HisGly: 2.496 ± 0.026	0.709HisHis: 0.709 ± 0.016	0.719HisIle: 0.719 ± 0.018	0.396HisLys: 0.396 ± 0.011	2.477HisLeu: 2.477 ± 0.034	0.361HisMet: 0.361 ± 0.011	0.418HisAsn: 0.418 ± 0.011	1.898HisPro: 1.898 ± 0.03	0.664HisGln: 0.664 ± 0.015	2.142HisArg: 2.142 ± 0.029	1.107HisSer: 1.107 ± 0.02	1.407HisThr: 1.407 ± 0.023	1.801HisVal: 1.801 ± 0.024	0.397HisTrp: 0.397 ± 0.013	0.516HisTyr: 0.516 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.809IleAla: 4.809 ± 0.048	0.297IleCys: 0.297 ± 0.009	2.255IleAsp: 2.255 ± 0.03	1.969IleGlu: 1.969 ± 0.028	0.69IlePhe: 0.69 ± 0.017	3.54IleGly: 3.54 ± 0.036	0.66IleHis: 0.66 ± 0.017	0.874IleIle: 0.874 ± 0.022	0.792IleLys: 0.792 ± 0.018	2.464IleLeu: 2.464 ± 0.033	0.442IleMet: 0.442 ± 0.014	0.743IleAsn: 0.743 ± 0.019	1.874IlePro: 1.874 ± 0.025	0.759IleGln: 0.759 ± 0.017	2.366IleArg: 2.366 ± 0.028	1.73IleSer: 1.73 ± 0.027	2.29IleThr: 2.29 ± 0.032	2.728IleVal: 2.728 ± 0.033	0.417IleTrp: 0.417 ± 0.01	0.547IleTyr: 0.547 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.138LysAla: 3.138 ± 0.041	0.128LysCys: 0.128 ± 0.007	1.411LysAsp: 1.411 ± 0.026	1.232LysGlu: 1.232 ± 0.023	0.483LysPhe: 0.483 ± 0.014	1.943LysGly: 1.943 ± 0.033	0.467LysHis: 0.467 ± 0.013	0.906LysIle: 0.906 ± 0.016	0.936LysLys: 0.936 ± 0.027	2.105LysLeu: 2.105 ± 0.029	0.39LysMet: 0.39 ± 0.013	0.598LysAsn: 0.598 ± 0.017	1.444LysPro: 1.444 ± 0.028	0.789LysGln: 0.789 ± 0.018	1.429LysArg: 1.429 ± 0.025	1.235LysSer: 1.235 ± 0.019	1.386LysThr: 1.386 ± 0.026	2.034LysVal: 2.034 ± 0.03	0.314LysTrp: 0.314 ± 0.01	0.521LysTyr: 0.521 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.836LeuAla: 14.836 ± 0.102	0.896LeuCys: 0.896 ± 0.018	6.582LeuAsp: 6.582 ± 0.048	4.633LeuGlu: 4.633 ± 0.043	2.662LeuPhe: 2.662 ± 0.033	9.114LeuGly: 9.114 ± 0.056	2.417LeuHis: 2.417 ± 0.03	3.279LeuIle: 3.279 ± 0.042	2.209LeuLys: 2.209 ± 0.032	11.271LeuLeu: 11.271 ± 0.089	1.633LeuMet: 1.633 ± 0.026	1.767LeuAsn: 1.767 ± 0.029	6.528LeuPro: 6.528 ± 0.058	2.442LeuGln: 2.442 ± 0.029	8.471LeuArg: 8.471 ± 0.068	5.326LeuSer: 5.326 ± 0.043	6.968LeuThr: 6.968 ± 0.047	8.779LeuVal: 8.779 ± 0.064	1.346LeuTrp: 1.346 ± 0.022	1.925LeuTyr: 1.925 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.185MetAla: 2.185 ± 0.03	0.139MetCys: 0.139 ± 0.007	0.866MetAsp: 0.866 ± 0.017	0.737MetGlu: 0.737 ± 0.017	0.448MetPhe: 0.448 ± 0.012	1.314MetGly: 1.314 ± 0.024	0.375MetHis: 0.375 ± 0.01	0.648MetIle: 0.648 ± 0.016	0.449MetLys: 0.449 ± 0.014	1.64MetLeu: 1.64 ± 0.024	0.276MetMet: 0.276 ± 0.011	0.452MetAsn: 0.452 ± 0.012	1.125MetPro: 1.125 ± 0.019	0.47MetGln: 0.47 ± 0.014	1.375MetArg: 1.375 ± 0.024	1.358MetSer: 1.358 ± 0.024	1.589MetThr: 1.589 ± 0.026	1.283MetVal: 1.283 ± 0.021	0.223MetTrp: 0.223 ± 0.008	0.33MetTyr: 0.33 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.283AsnAla: 2.283 ± 0.032	0.181AsnCys: 0.181 ± 0.008	1.028AsnAsp: 1.028 ± 0.018	0.849AsnGlu: 0.849 ± 0.016	0.53AsnPhe: 0.53 ± 0.015	2.079AsnGly: 2.079 ± 0.03	0.436AsnHis: 0.436 ± 0.013	0.697AsnIle: 0.697 ± 0.018	0.468AsnLys: 0.468 ± 0.012	1.713AsnLeu: 1.713 ± 0.028	0.301AsnMet: 0.301 ± 0.01	0.499AsnAsn: 0.499 ± 0.016	1.422AsnPro: 1.422 ± 0.022	0.592AsnGln: 0.592 ± 0.016	1.339AsnArg: 1.339 ± 0.021	1.094AsnSer: 1.094 ± 0.025	1.208AsnThr: 1.208 ± 0.024	1.442AsnVal: 1.442 ± 0.023	0.334AsnTrp: 0.334 ± 0.012	0.455AsnTyr: 0.455 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.379ProAla: 8.379 ± 0.068	0.371ProCys: 0.371 ± 0.013	4.441ProAsp: 4.441 ± 0.038	4.247ProGlu: 4.247 ± 0.043	1.544ProPhe: 1.544 ± 0.022	6.362ProGly: 6.362 ± 0.053	1.441ProHis: 1.441 ± 0.024	1.31ProIle: 1.31 ± 0.024	1.279ProLys: 1.279 ± 0.022	5.381ProLeu: 5.381 ± 0.051	0.997ProMet: 0.997 ± 0.019	0.958ProAsn: 0.958 ± 0.02	3.433ProPro: 3.433 ± 0.051	1.919ProGln: 1.919 ± 0.035	3.804ProArg: 3.804 ± 0.041	3.333ProSer: 3.333 ± 0.038	3.287ProThr: 3.287 ± 0.038	5.433ProVal: 5.433 ± 0.048	0.92ProTrp: 0.92 ± 0.018	1.429ProTyr: 1.429 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.921GlnAla: 3.921 ± 0.043	0.192GlnCys: 0.192 ± 0.008	1.515GlnAsp: 1.515 ± 0.024	1.526GlnGlu: 1.526 ± 0.027	0.74GlnPhe: 0.74 ± 0.017	2.431GlnGly: 2.431 ± 0.031	0.737GlnHis: 0.737 ± 0.015	1.139GlnIle: 1.139 ± 0.024	0.656GlnLys: 0.656 ± 0.018	3.236GlnLeu: 3.236 ± 0.037	0.504GlnMet: 0.504 ± 0.013	0.566GlnAsn: 0.566 ± 0.016	1.842GlnPro: 1.842 ± 0.031	1.378GlnGln: 1.378 ± 0.029	2.392GlnArg: 2.392 ± 0.027	1.415GlnSer: 1.415 ± 0.021	1.491GlnThr: 1.491 ± 0.023	2.472GlnVal: 2.472 ± 0.031	0.53GlnTrp: 0.53 ± 0.015	0.663GlnTyr: 0.663 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.297ArgAla: 9.297 ± 0.079	0.598ArgCys: 0.598 ± 0.014	4.033ArgAsp: 4.033 ± 0.039	4.612ArgGlu: 4.612 ± 0.042	2.3ArgPhe: 2.3 ± 0.027	5.574ArgGly: 5.574 ± 0.053	2.153ArgHis: 2.153 ± 0.026	3.278ArgIle: 3.278 ± 0.033	1.711ArgLys: 1.711 ± 0.026	8.726ArgLeu: 8.726 ± 0.069	1.671ArgMet: 1.671 ± 0.021	1.409ArgAsn: 1.409 ± 0.021	4.902ArgPro: 4.902 ± 0.051	2.422ArgGln: 2.422 ± 0.033	7.541ArgArg: 7.541 ± 0.062	4.093ArgSer: 4.093 ± 0.037	5.277ArgThr: 5.277 ± 0.044	5.614ArgVal: 5.614 ± 0.045	1.387ArgTrp: 1.387 ± 0.024	1.782ArgTyr: 1.782 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.931SerAla: 6.931 ± 0.05	0.443SerCys: 0.443 ± 0.012	2.861SerAsp: 2.861 ± 0.03	2.464SerGlu: 2.464 ± 0.033	1.58SerPhe: 1.58 ± 0.025	6.18SerGly: 6.18 ± 0.061	1.08SerHis: 1.08 ± 0.019	1.523SerIle: 1.523 ± 0.024	1.154SerLys: 1.154 ± 0.021	4.998SerLeu: 4.998 ± 0.039	1.132SerMet: 1.132 ± 0.019	1.009SerAsn: 1.009 ± 0.019	3.286SerPro: 3.286 ± 0.041	1.362SerGln: 1.362 ± 0.023	3.679SerArg: 3.679 ± 0.043	3.198SerSer: 3.198 ± 0.044	3.259SerThr: 3.259 ± 0.039	4.386SerVal: 4.386 ± 0.034	0.958SerTrp: 0.958 ± 0.019	1.321SerTyr: 1.321 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	8.991ThrAla: 8.991 ± 0.062	0.468ThrCys: 0.468 ± 0.014	3.646ThrAsp: 3.646 ± 0.039	3.23ThrGlu: 3.23 ± 0.033	1.677ThrPhe: 1.677 ± 0.027	6.748ThrGly: 6.748 ± 0.05	1.288ThrHis: 1.288 ± 0.022	1.741ThrIle: 1.741 ± 0.027	1.267ThrLys: 1.267 ± 0.026	5.753ThrLeu: 5.753 ± 0.045	0.976ThrMet: 0.976 ± 0.019	1.119ThrAsn: 1.119 ± 0.025	4.168ThrPro: 4.168 ± 0.044	1.475ThrGln: 1.475 ± 0.021	3.901ThrArg: 3.901 ± 0.037	3.389ThrSer: 3.389 ± 0.036	3.979ThrThr: 3.979 ± 0.05	6.112ThrVal: 6.112 ± 0.047	0.964ThrTrp: 0.964 ± 0.019	1.446ThrTyr: 1.446 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	10.537ValAla: 10.537 ± 0.071	0.749ValCys: 0.749 ± 0.017	4.925ValAsp: 4.925 ± 0.042	4.652ValGlu: 4.652 ± 0.045	2.435ValPhe: 2.435 ± 0.031	6.445ValGly: 6.445 ± 0.05	1.991ValHis: 1.991 ± 0.027	2.871ValIle: 2.871 ± 0.034	1.831ValLys: 1.831 ± 0.034	9.415ValLeu: 9.415 ± 0.082	1.405ValMet: 1.405 ± 0.024	1.763ValAsn: 1.763 ± 0.029	5.107ValPro: 5.107 ± 0.044	2.245ValGln: 2.245 ± 0.024	6.972ValArg: 6.972 ± 0.055	4.492ValSer: 4.492 ± 0.044	5.642ValThr: 5.642 ± 0.038	7.864ValVal: 7.864 ± 0.065	1.201ValTrp: 1.201 ± 0.024	1.621ValTyr: 1.621 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.709TrpAla: 1.709 ± 0.028	0.155TrpCys: 0.155 ± 0.007	0.866TrpAsp: 0.866 ± 0.017	0.713TrpGlu: 0.713 ± 0.016	0.513TrpPhe: 0.513 ± 0.012	1.144TrpGly: 1.144 ± 0.023	0.399TrpHis: 0.399 ± 0.013	0.576TrpIle: 0.576 ± 0.012	0.412TrpLys: 0.412 ± 0.012	1.806TrpLeu: 1.806 ± 0.029	0.302TrpMet: 0.302 ± 0.009	0.453TrpAsn: 0.453 ± 0.014	0.831TrpPro: 0.831 ± 0.017	0.651TrpGln: 0.651 ± 0.016	1.365TrpArg: 1.365 ± 0.019	1.016TrpSer: 1.016 ± 0.02	1.085TrpThr: 1.085 ± 0.02	0.992TrpVal: 0.992 ± 0.017	0.346TrpTrp: 0.346 ± 0.01	0.391TrpTyr: 0.391 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.806TyrAla: 2.806 ± 0.033	0.193TyrCys: 0.193 ± 0.008	1.603TyrAsp: 1.603 ± 0.028	1.307TyrGlu: 1.307 ± 0.022	0.682TyrPhe: 0.682 ± 0.015	2.357TyrGly: 2.357 ± 0.035	0.411TyrHis: 0.411 ± 0.012	0.522TyrIle: 0.522 ± 0.013	0.457TyrLys: 0.457 ± 0.014	2.092TyrLeu: 2.092 ± 0.03	0.271TyrMet: 0.271 ± 0.01	0.455TyrAsn: 0.455 ± 0.014	1.083TyrPro: 1.083 ± 0.022	0.651TyrGln: 0.651 ± 0.015	1.83TyrArg: 1.83 ± 0.024	1.052TyrSer: 1.052 ± 0.019	1.242TyrThr: 1.242 ± 0.021	1.715TyrVal: 1.715 ± 0.025	0.379TyrTrp: 0.379 ± 0.013	0.488TyrTyr: 0.488 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9364 proteins (3091077 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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