Rhodococcus sp. ABRD24

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1A0Z1|A0A4V1A0Z1_9NOCA Multicopper oxidase family protein OS=Rhodococcus sp. ABRD24 OX=2507582 GN=ERC79_10020 PE=4 SV=1
MM1 pKa = 7.05QNKK4 pKa = 6.23TTRR7 pKa = 11.84STVLAAVAALALAGGGVANAAEE29 pKa = 4.77GSSGSLSGSSGSSGSSTGSSATPGTPYY56 pKa = 10.57EE57 pKa = 4.11FEE59 pKa = 4.1NWDD62 pKa = 3.62TCQLDD67 pKa = 4.02LVYY70 pKa = 11.26NPVTDD75 pKa = 5.19LGTCMTVIIRR85 pKa = 11.84DD86 pKa = 2.94GDD88 pKa = 3.64MRR90 pKa = 11.84IGNLDD95 pKa = 3.27VAVPDD100 pKa = 4.24GSLMIAGGVSQDD112 pKa = 3.85ADD114 pKa = 3.74DD115 pKa = 4.18PTIQTFVPAADD126 pKa = 3.85DD127 pKa = 4.11GKK129 pKa = 10.95FGVYY133 pKa = 10.23ANPITVPGGAFGAASAEE150 pKa = 4.23NFGPTAIQATVEE162 pKa = 4.26AVALPAVDD170 pKa = 4.78PYY172 pKa = 11.64NLAVQLPVRR181 pKa = 11.84MKK183 pKa = 10.86LSNPLLGDD191 pKa = 3.14NCYY194 pKa = 10.14IGSAANPINLSLDD207 pKa = 3.39LVEE210 pKa = 5.71AGTAEE215 pKa = 4.37WISTNGPDD223 pKa = 3.64VPGGVWPQATHH234 pKa = 6.13EE235 pKa = 4.33AADD238 pKa = 3.83FAVPGATGCGPLGSLNWAVNLRR260 pKa = 11.84AGLPSAGTGNSLSTTSAVYY279 pKa = 10.19NVAGWEE285 pKa = 3.97LLEE288 pKa = 4.25PP289 pKa = 4.68

Molecular weight: 28.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6U6B2|A0A4P6U6B2_9NOCA DNA-binding protein OS=Rhodococcus sp. ABRD24 OX=2507582 GN=ERC79_02070 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4379

0

4379

1486773

27

8958

339.5

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.836 ± 0.047

0.781 ± 0.011

6.204 ± 0.031

5.543 ± 0.035

2.996 ± 0.02

9.092 ± 0.037

2.133 ± 0.018

4.259 ± 0.026

2.031 ± 0.029

10.025 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.971 ± 0.016

2.042 ± 0.02

5.572 ± 0.027

2.778 ± 0.017

7.371 ± 0.038

5.818 ± 0.022

6.168 ± 0.026

8.967 ± 0.038

1.431 ± 0.013

1.98 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski