Carpinus fangiana
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6RI55|A0A5N6RI55_9ROSI Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_017081 PE=3 SV=1
MM1 pKa = 7.81 DD2 pKa = 4.07 VSSCLLFEE10 pKa = 6.08 DD11 pKa = 4.49 IADD14 pKa = 4.14 SEE16 pKa = 4.37 TDD18 pKa = 3.41 AAGPDD23 pKa = 3.9 HH24 pKa = 7.32 KK25 pKa = 10.85 IHH27 pKa = 7.66 ADD29 pKa = 3.53 DD30 pKa = 4.93 DD31 pKa = 4.03 QDD33 pKa = 5.63 AEE35 pKa = 4.52 SCSCDD40 pKa = 3.84 DD41 pKa = 4.72 ACDD44 pKa = 3.79 DD45 pKa = 3.57 HH46 pKa = 6.73 HH47 pKa = 7.21 TPGFDD52 pKa = 3.12 EE53 pKa = 6.39 ALDD56 pKa = 4.17 CDD58 pKa = 4.36 EE59 pKa = 5.26 KK60 pKa = 11.58 DD61 pKa = 3.39 SGASNGGDD69 pKa = 3.22 AGDD72 pKa = 4.73 DD73 pKa = 3.28 HH74 pKa = 6.82 HH75 pKa = 7.58 QCGRR79 pKa = 11.84 PCASCCLSSDD89 pKa = 3.41 HH90 pKa = 6.93 AALGSLSSEE99 pKa = 3.88 EE100 pKa = 4.48 GEE102 pKa = 4.54 GEE104 pKa = 4.07 EE105 pKa = 4.17 EE106 pKa = 4.42 TNMGEE111 pKa = 4.31 INKK114 pKa = 9.82 DD115 pKa = 3.4 DD116 pKa = 4.1 PSKK119 pKa = 10.91 KK120 pKa = 10.16 KK121 pKa = 10.44 EE122 pKa = 3.84 INKK125 pKa = 10.43 DD126 pKa = 3.38 EE127 pKa = 4.33 MEE129 pKa = 5.97 DD130 pKa = 3.54 GLFWEE135 pKa = 4.6 TCMAVGYY142 pKa = 8.94 PP143 pKa = 3.59
Molecular weight: 15.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.516
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A5N6QZ34|A0A5N6QZ34_9ROSI Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_007833 PE=4 SV=1
MM1 pKa = 7.47 PRR3 pKa = 11.84 HH4 pKa = 6.18 RR5 pKa = 11.84 PPLLQRR11 pKa = 11.84 RR12 pKa = 11.84 PQPRR16 pKa = 11.84 SLLSSQSLSSSLLSLTHH33 pKa = 5.97 SLSRR37 pKa = 11.84 SSAVALSPARR47 pKa = 11.84 QPPLSLPLASRR58 pKa = 11.84 SSLFHH63 pKa = 6.56 SPAAALSPARR73 pKa = 4.24
Molecular weight: 7.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27308
1231
28539
12421239
50
6047
435.2
48.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.619 ± 0.016
1.749 ± 0.007
5.343 ± 0.01
6.315 ± 0.015
3.996 ± 0.009
6.827 ± 0.013
2.458 ± 0.006
4.998 ± 0.01
5.591 ± 0.012
9.55 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.006
4.043 ± 0.009
5.331 ± 0.017
3.758 ± 0.01
5.723 ± 0.013
8.792 ± 0.016
5.08 ± 0.009
6.484 ± 0.011
1.336 ± 0.005
2.669 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here