Aggregicoccus sp. 17bor-14
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5829 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I2GT76|A0A6I2GT76_9DELT GGDEF domain-containing protein OS=Aggregicoccus sp. 17bor-14 OX=2583808 GN=FGE12_25110 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 AALLCAGLLALAGCGGSPDD21 pKa = 4.14 PEE23 pKa = 4.19 PVKK26 pKa = 9.29 PTPPVTPAPPVVVDD40 pKa = 4.28 ADD42 pKa = 3.78 HH43 pKa = 7.51 DD44 pKa = 4.61 GVPSTADD51 pKa = 3.65 CADD54 pKa = 4.19 DD55 pKa = 4.1 DD56 pKa = 4.12 ATRR59 pKa = 11.84 FQYY62 pKa = 11.15 VSGHH66 pKa = 6.47 RR67 pKa = 11.84 DD68 pKa = 3.01 ADD70 pKa = 3.85 GDD72 pKa = 4.42 GVGADD77 pKa = 3.91 ALEE80 pKa = 4.23 QVCAGAALPQGWVSTGGDD98 pKa = 3.74 CATYY102 pKa = 10.7 DD103 pKa = 3.31 ATRR106 pKa = 11.84 WRR108 pKa = 11.84 EE109 pKa = 3.67 LAVYY113 pKa = 9.49 EE114 pKa = 4.6 DD115 pKa = 3.76 WDD117 pKa = 3.76 GDD119 pKa = 3.57 GRR121 pKa = 11.84 TRR123 pKa = 11.84 PYY125 pKa = 11.19 AQTLCIGAQVPTGYY139 pKa = 8.47 VTQRR143 pKa = 11.84 GEE145 pKa = 4.15 DD146 pKa = 3.53 DD147 pKa = 4.94 CSDD150 pKa = 3.73 FDD152 pKa = 3.38 ATAWHH157 pKa = 6.27 EE158 pKa = 4.24 VPLYY162 pKa = 10.7 FDD164 pKa = 4.91 LDD166 pKa = 3.75 GDD168 pKa = 4.76 GVGDD172 pKa = 4.29 DD173 pKa = 4.64 YY174 pKa = 12.1 AMSMCLGSAPPPTYY188 pKa = 9.96 MVATGGDD195 pKa = 3.97 CAPRR199 pKa = 11.84 DD200 pKa = 3.61 ATLYY204 pKa = 10.58 TMLPYY209 pKa = 10.3 AYY211 pKa = 9.4 RR212 pKa = 11.84 DD213 pKa = 3.47 ADD215 pKa = 3.64 GDD217 pKa = 4.16 GATVPQQGSVCSGFYY232 pKa = 10.76 LPAGYY237 pKa = 9.86 RR238 pKa = 11.84 EE239 pKa = 4.22 SAQGLDD245 pKa = 4.71 CNDD248 pKa = 4.39 ADD250 pKa = 3.86 PSVYY254 pKa = 10.76 SMQPGFPDD262 pKa = 3.85 PDD264 pKa = 4.08 GDD266 pKa = 4.2 GVGSGEE272 pKa = 4.21 SFEE275 pKa = 4.53 VCAGVAMPRR284 pKa = 11.84 YY285 pKa = 9.17 SSRR288 pKa = 11.84 RR289 pKa = 11.84 SDD291 pKa = 3.32 DD292 pKa = 3.78 CAPQDD297 pKa = 3.5 SSRR300 pKa = 11.84 WEE302 pKa = 3.64 QRR304 pKa = 11.84 EE305 pKa = 3.82 YY306 pKa = 11.38 RR307 pKa = 11.84 LGDD310 pKa = 3.36 ADD312 pKa = 3.82 GDD314 pKa = 4.01 GRR316 pKa = 11.84 TVPLAEE322 pKa = 4.28 PASFCVGNTDD332 pKa = 3.18 PQGYY336 pKa = 9.63 SRR338 pKa = 11.84 GTPWPDD344 pKa = 3.35 DD345 pKa = 4.19 CDD347 pKa = 4.01 DD348 pKa = 4.42 ADD350 pKa = 3.54 AARR353 pKa = 11.84 YY354 pKa = 8.42 QVLAYY359 pKa = 10.09 AYY361 pKa = 10.04 RR362 pKa = 11.84 DD363 pKa = 3.5 ADD365 pKa = 3.59 GDD367 pKa = 4.21 GATVPATGSLCSGASLPAGYY387 pKa = 8.17 ATQSRR392 pKa = 11.84 GADD395 pKa = 3.64 CDD397 pKa = 4.08 DD398 pKa = 4.7 ADD400 pKa = 3.72 AQRR403 pKa = 11.84 FVQLSGFADD412 pKa = 3.39 VDD414 pKa = 3.72 ADD416 pKa = 3.94 GVGAGEE422 pKa = 4.03 AQAFCTAGALPAGFVASSTDD442 pKa = 3.62 CAAQDD447 pKa = 3.38 AARR450 pKa = 11.84 WRR452 pKa = 11.84 TVTPGFLDD460 pKa = 3.28 QDD462 pKa = 3.56 GDD464 pKa = 4.11 GYY466 pKa = 9.48 TVVDD470 pKa = 4.41 PAPTAQCIGTAPEE483 pKa = 4.32 APSVLAARR491 pKa = 11.84 GNDD494 pKa = 4.27 CEE496 pKa = 4.7 DD497 pKa = 3.51 TDD499 pKa = 3.74 PTRR502 pKa = 11.84 FLWRR506 pKa = 11.84 VFYY509 pKa = 10.68 RR510 pKa = 11.84 DD511 pKa = 3.2 EE512 pKa = 6.4 DD513 pKa = 4.37 GDD515 pKa = 4.45 GVGAAPRR522 pKa = 11.84 LLRR525 pKa = 11.84 CLATGAAPAGEE536 pKa = 4.7 SPYY539 pKa = 11.33 GWDD542 pKa = 5.03 SDD544 pKa = 4.27 DD545 pKa = 5.07 ADD547 pKa = 3.94 PAVQQSEE554 pKa = 4.07 EE555 pKa = 4.3 DD556 pKa = 3.73 EE557 pKa = 5.08 AVLQLLLEE565 pKa = 4.42 TT566 pKa = 4.86
Molecular weight: 58.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.541
Grimsley 3.376
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.151
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.846
Patrickios 0.439
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A6I2GLG1|A0A6I2GLG1_9DELT TIGR02266 family protein OS=Aggregicoccus sp. 17bor-14 OX=2583808 GN=FGE12_00710 PE=4 SV=1
MM1 pKa = 7.76 AARR4 pKa = 11.84 KK5 pKa = 7.51 TSRR8 pKa = 11.84 KK9 pKa = 8.11 STAKK13 pKa = 10.09 RR14 pKa = 11.84 STGTRR19 pKa = 11.84 SKK21 pKa = 10.82 RR22 pKa = 11.84 SGGAKK27 pKa = 9.55 RR28 pKa = 11.84 RR29 pKa = 11.84 TGGTAAKK36 pKa = 9.97 RR37 pKa = 11.84 ATAKK41 pKa = 10.47 RR42 pKa = 11.84 STAKK46 pKa = 10.4 RR47 pKa = 11.84 GTARR51 pKa = 11.84 KK52 pKa = 9.0 SASRR56 pKa = 11.84 GKK58 pKa = 8.93 RR59 pKa = 11.84 TAKK62 pKa = 10.2 RR63 pKa = 11.84 STAGKK68 pKa = 9.63 RR69 pKa = 11.84 VTARR73 pKa = 11.84 KK74 pKa = 8.82 PGARR78 pKa = 11.84 RR79 pKa = 11.84 TAAKK83 pKa = 8.34 RR84 pKa = 11.84 TSTRR88 pKa = 11.84 RR89 pKa = 11.84 ARR91 pKa = 11.84 RR92 pKa = 3.31
Molecular weight: 9.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.454
IPC2_protein 11.594
IPC_protein 13.188
Toseland 13.349
ProMoST 13.846
Dawson 13.349
Bjellqvist 13.349
Wikipedia 13.832
Rodwell 13.056
Grimsley 13.393
Solomon 13.846
Lehninger 13.744
Nozaki 13.349
DTASelect 13.349
Thurlkill 13.349
EMBOSS 13.846
Sillero 13.349
Patrickios 12.764
IPC_peptide 13.846
IPC2_peptide 12.837
IPC2.peptide.svr19 9.446
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5829
0
5829
2075293
38
4099
356.0
38.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.817 ± 0.055
0.871 ± 0.015
4.643 ± 0.023
6.348 ± 0.038
3.174 ± 0.02
9.064 ± 0.033
2.082 ± 0.016
2.416 ± 0.027
2.594 ± 0.031
11.991 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.645 ± 0.013
1.798 ± 0.019
6.227 ± 0.033
3.591 ± 0.021
8.38 ± 0.041
5.215 ± 0.025
4.85 ± 0.028
8.001 ± 0.028
1.243 ± 0.015
2.049 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here