Escherichia phage nom

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Vequintavirus; unclassified Vequintavirus

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9XH99|A0A6B9XH99_9CAUD Uncharacterized protein OS=Escherichia phage nom OX=2696434 GN=nom_140 PE=4 SV=1
MM1 pKa = 7.21SHH3 pKa = 5.63NSKK6 pKa = 10.11WKK8 pKa = 10.08DD9 pKa = 3.03LGFASRR15 pKa = 11.84AEE17 pKa = 4.05YY18 pKa = 10.81VGWLWFNGLTNDD30 pKa = 2.83IKK32 pKa = 10.85MYY34 pKa = 10.94DD35 pKa = 3.37DD36 pKa = 3.82VYY38 pKa = 10.64YY39 pKa = 10.79DD40 pKa = 3.95EE41 pKa = 4.54YY42 pKa = 9.79TGKK45 pKa = 10.35IINISVEE52 pKa = 3.99EE53 pKa = 4.12DD54 pKa = 3.28TFIQQDD60 pKa = 3.24TDD62 pKa = 3.45TCLDD66 pKa = 3.57KK67 pKa = 11.34YY68 pKa = 10.71VSEE71 pKa = 4.33VEE73 pKa = 4.15EE74 pKa = 4.43ANN76 pKa = 3.5

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9XG33|A0A6B9XG33_9CAUD DNA polymerase OS=Escherichia phage nom OX=2696434 GN=nom_199 PE=3 SV=1
MM1 pKa = 7.81KK2 pKa = 10.33INFEE6 pKa = 4.46KK7 pKa = 10.36IVKK10 pKa = 9.65RR11 pKa = 11.84SHH13 pKa = 6.58ASEE16 pKa = 4.09EE17 pKa = 4.14LPEE20 pKa = 3.9RR21 pKa = 11.84RR22 pKa = 11.84QRR24 pKa = 11.84NNKK27 pKa = 8.77LNKK30 pKa = 8.62PKK32 pKa = 10.53RR33 pKa = 11.84FGKK36 pKa = 9.67IWRR39 pKa = 11.84NHH41 pKa = 5.54YY42 pKa = 9.72DD43 pKa = 3.21RR44 pKa = 11.84KK45 pKa = 10.86LPGLLLEE52 pKa = 4.87RR53 pKa = 11.84EE54 pKa = 4.29RR55 pKa = 11.84QAEE58 pKa = 4.29RR59 pKa = 11.84YY60 pKa = 7.52RR61 pKa = 11.84HH62 pKa = 5.78KK63 pKa = 11.17GKK65 pKa = 10.56

Molecular weight: 8.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

208

0

208

40161

32

1875

193.1

21.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.972 ± 0.188

1.646 ± 0.085

6.845 ± 0.13

7.101 ± 0.278

4.235 ± 0.094

7.009 ± 0.215

1.977 ± 0.078

5.871 ± 0.133

6.92 ± 0.234

7.677 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.101

4.995 ± 0.169

3.85 ± 0.134

3.23 ± 0.127

4.499 ± 0.113

5.513 ± 0.14

5.657 ± 0.207

7.134 ± 0.136

1.793 ± 0.09

4.221 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski