Cyanidioschyzon merolae (strain 10D) (Red alga)

Taxonomy: cellular organisms; Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae; Cyanidioschyzon; Cyanidioschyzon merolae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q85G19|Q85G19_CYAM1 Ycf40 protein OS=Cyanidioschyzon merolae (strain 10D) OX=280699 GN=ycf40 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 10.23KK3 pKa = 9.74IQLVNQKK10 pKa = 10.08EE11 pKa = 4.58GIDD14 pKa = 3.7VTIQCAGDD22 pKa = 3.73QYY24 pKa = 11.48ILDD27 pKa = 3.98AAEE30 pKa = 4.09EE31 pKa = 4.17QGVDD35 pKa = 3.62LPYY38 pKa = 10.51SCRR41 pKa = 11.84AGACSTCAGKK51 pKa = 10.02LVKK54 pKa = 10.7GSVDD58 pKa = 3.25QSDD61 pKa = 3.39QSFLDD66 pKa = 3.48EE67 pKa = 4.45DD68 pKa = 4.05QISKK72 pKa = 10.78GFILTCVAYY81 pKa = 7.23PTSDD85 pKa = 3.65CVIQTHH91 pKa = 5.54QEE93 pKa = 3.65EE94 pKa = 4.2ALYY97 pKa = 10.88

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1V5Y0|M1V5Y0_CYAM1 DUF1995 domain-containing protein OS=Cyanidioschyzon merolae (strain 10D) OX=280699 GN=CYME_CMO077C PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.97RR15 pKa = 11.84KK16 pKa = 8.56RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.39

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4995

0

4995

2516387

25

6020

503.8

55.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.003 ± 0.041

1.773 ± 0.016

4.899 ± 0.021

6.298 ± 0.03

3.301 ± 0.022

6.398 ± 0.028

2.49 ± 0.016

3.695 ± 0.022

2.813 ± 0.025

10.32 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.849 ± 0.012

2.582 ± 0.018

5.504 ± 0.029

4.339 ± 0.02

8.684 ± 0.028

7.877 ± 0.038

5.589 ± 0.032

6.747 ± 0.022

1.532 ± 0.015

2.305 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski