Staphylococcus phage StB20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9A136|H9A136_9CAUD Uncharacterized protein OS=Staphylococcus phage StB20 OX=1147043 PE=4 SV=1
MM1 pKa = 7.48IIKK4 pKa = 9.28MGEE7 pKa = 4.13VEE9 pKa = 4.1THH11 pKa = 5.32INPRR15 pKa = 11.84NVDD18 pKa = 2.84IGDD21 pKa = 3.47IGSRR25 pKa = 11.84FYY27 pKa = 11.1TEE29 pKa = 4.84DD30 pKa = 3.4EE31 pKa = 3.81NTAFIRR37 pKa = 11.84IRR39 pKa = 11.84INYY42 pKa = 8.26NGSPVDD48 pKa = 3.83LTKK51 pKa = 10.78TDD53 pKa = 4.04MKK55 pKa = 10.73PKK57 pKa = 10.28LDD59 pKa = 4.12LFMEE63 pKa = 5.06DD64 pKa = 2.93GSVFIDD70 pKa = 3.4EE71 pKa = 4.83TIEE74 pKa = 3.88VLIPEE79 pKa = 4.76IGLIKK84 pKa = 10.39YY85 pKa = 7.56DD86 pKa = 3.39IPSNVIKK93 pKa = 10.76HH94 pKa = 5.58IGKK97 pKa = 9.58VNCKK101 pKa = 10.24LFMDD105 pKa = 4.14SLEE108 pKa = 4.64HH109 pKa = 6.13SVHH112 pKa = 5.37VANFSFTIVDD122 pKa = 3.71SGVEE126 pKa = 3.71EE127 pKa = 4.33AVAKK131 pKa = 9.68EE132 pKa = 3.88VNLNIVKK139 pKa = 9.1DD140 pKa = 3.84TVKK143 pKa = 10.76QIMSEE148 pKa = 4.09DD149 pKa = 3.76LVDD152 pKa = 4.66LLNDD156 pKa = 3.75GFKK159 pKa = 11.11DD160 pKa = 3.39KK161 pKa = 10.67LTGDD165 pKa = 4.03LEE167 pKa = 4.89NYY169 pKa = 10.29LSTHH173 pKa = 5.32NEE175 pKa = 3.68EE176 pKa = 4.48FKK178 pKa = 11.15GPKK181 pKa = 8.91GDD183 pKa = 4.28KK184 pKa = 10.59GPQGEE189 pKa = 4.41QGPQGLQGEE198 pKa = 4.34QGIQGEE204 pKa = 4.55KK205 pKa = 10.64GEE207 pKa = 4.51QGPQGATGPQGPPGKK222 pKa = 9.91DD223 pKa = 2.99GRR225 pKa = 11.84DD226 pKa = 3.61GVDD229 pKa = 3.2GKK231 pKa = 11.42SFAYY235 pKa = 10.31DD236 pKa = 3.42DD237 pKa = 4.07FTPEE241 pKa = 4.13QLDD244 pKa = 3.49KK245 pKa = 11.32LKK247 pKa = 10.9PEE249 pKa = 4.82LPDD252 pKa = 3.74FSKK255 pKa = 8.78WQKK258 pKa = 11.08YY259 pKa = 9.85KK260 pKa = 10.09LTNDD264 pKa = 4.49DD265 pKa = 3.88GNQIATFDD273 pKa = 3.58SDD275 pKa = 4.19NRR277 pKa = 11.84MTNEE281 pKa = 4.13NLLSTPTGFYY291 pKa = 11.0YY292 pKa = 10.54NYY294 pKa = 10.2EE295 pKa = 4.09YY296 pKa = 10.9DD297 pKa = 3.88DD298 pKa = 4.53SPNGSEE304 pKa = 3.88GVVYY308 pKa = 10.94VMEE311 pKa = 4.3YY312 pKa = 10.21TDD314 pKa = 3.53EE315 pKa = 4.3CKK317 pKa = 10.21KK318 pKa = 10.85VYY320 pKa = 9.99YY321 pKa = 10.13SSYY324 pKa = 10.36RR325 pKa = 11.84YY326 pKa = 9.94NDD328 pKa = 4.11LYY330 pKa = 11.42VKK332 pKa = 9.67TYY334 pKa = 10.57HH335 pKa = 7.45PDD337 pKa = 3.54FGWLEE342 pKa = 3.93WEE344 pKa = 4.74EE345 pKa = 3.94ISNGYY350 pKa = 7.89TDD352 pKa = 4.0TGWIPLAIKK361 pKa = 10.37NDD363 pKa = 3.62YY364 pKa = 10.77EE365 pKa = 5.27IITNLNYY372 pKa = 8.77EE373 pKa = 3.61QSYY376 pKa = 9.94RR377 pKa = 11.84VIDD380 pKa = 3.43YY381 pKa = 11.49GNFKK385 pKa = 10.17KK386 pKa = 11.05VSVRR390 pKa = 11.84LGVIDD395 pKa = 4.32NSPSNHH401 pKa = 5.79SVVASIPEE409 pKa = 4.0YY410 pKa = 10.77LNPYY414 pKa = 10.17DD415 pKa = 3.53VFGYY419 pKa = 10.42GVSTTQGGRR428 pKa = 11.84PVTVTIVDD436 pKa = 3.97GDD438 pKa = 3.46IMLYY442 pKa = 10.41SNSFASSMNTYY453 pKa = 9.9YY454 pKa = 10.37QGEE457 pKa = 4.34WIII460 pKa = 4.24

Molecular weight: 51.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9A102|H9A102_9CAUD Uncharacterized protein OS=Staphylococcus phage StB20 OX=1147043 PE=4 SV=1
MM1 pKa = 7.45ILSKK5 pKa = 9.87TINQRR10 pKa = 11.84YY11 pKa = 9.27KK12 pKa = 11.01YY13 pKa = 8.05NTQGKK18 pKa = 7.18TPTEE22 pKa = 4.06VQRR25 pKa = 11.84EE26 pKa = 3.98LRR28 pKa = 11.84QLGVKK33 pKa = 10.18GFVVKK38 pKa = 10.62VAGNRR43 pKa = 11.84VTMKK47 pKa = 10.57VNEE50 pKa = 3.91NDD52 pKa = 2.91IKK54 pKa = 11.02KK55 pKa = 9.99NRR57 pKa = 11.84EE58 pKa = 3.66FLRR61 pKa = 4.98

Molecular weight: 7.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12593

34

620

193.7

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.289 ± 0.497

0.604 ± 0.099

6.313 ± 0.375

7.52 ± 0.508

3.947 ± 0.224

6.218 ± 0.506

1.803 ± 0.181

7.25 ± 0.328

9.426 ± 0.42

7.552 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.193

6.885 ± 0.365

3.121 ± 0.226

3.748 ± 0.212

4.066 ± 0.256

5.892 ± 0.282

6.003 ± 0.236

6.194 ± 0.174

1.247 ± 0.133

4.185 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski