Bacillus sp. CAG:988

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1397 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7F2U9|R7F2U9_9BACI Uncharacterized protein OS=Bacillus sp. CAG:988 OX=1262708 GN=BN822_00577 PE=4 SV=1
MM1 pKa = 6.63NQKK4 pKa = 10.37SVVRR8 pKa = 11.84KK9 pKa = 9.77NKK11 pKa = 9.39LFLWIGLAGVLVIILALVLIFVLDD35 pKa = 4.01GKK37 pKa = 10.82KK38 pKa = 10.32EE39 pKa = 4.14NNGSGTGQDD48 pKa = 3.59VLFEE52 pKa = 5.23DD53 pKa = 4.75IYY55 pKa = 11.41NNTVNATNYY64 pKa = 9.78KK65 pKa = 9.48IQFTISKK72 pKa = 9.48DD73 pKa = 3.37DD74 pKa = 3.49SSLVFTSNIDD84 pKa = 3.38DD85 pKa = 4.05SYY87 pKa = 12.09QEE89 pKa = 3.65ITEE92 pKa = 4.12QKK94 pKa = 5.96TTPEE98 pKa = 3.72EE99 pKa = 4.67SILSRR104 pKa = 11.84YY105 pKa = 8.46FVDD108 pKa = 3.69YY109 pKa = 11.47EE110 pKa = 4.24NMNLYY115 pKa = 10.12YY116 pKa = 10.77LSDD119 pKa = 4.03AGNMMDD125 pKa = 4.34HH126 pKa = 6.84FEE128 pKa = 4.75SIYY131 pKa = 10.36DD132 pKa = 3.65LKK134 pKa = 11.17SIYY137 pKa = 9.36TALNQNSAKK146 pKa = 10.62NVVDD150 pKa = 4.76DD151 pKa = 4.23DD152 pKa = 3.97TVEE155 pKa = 3.98YY156 pKa = 10.6VIPNLQAYY164 pKa = 10.03NYY166 pKa = 7.98MFTYY170 pKa = 10.73LDD172 pKa = 3.41IVKK175 pKa = 9.85EE176 pKa = 3.73IADD179 pKa = 3.77IDD181 pKa = 4.15FEE183 pKa = 5.39SITDD187 pKa = 3.68DD188 pKa = 3.85VVITVNSNGEE198 pKa = 4.2YY199 pKa = 10.39LSSLTFQLDD208 pKa = 3.79GVNFEE213 pKa = 4.8YY214 pKa = 11.1VFSDD218 pKa = 3.91FNAVTDD224 pKa = 3.81LTLPEE229 pKa = 4.21VTDD232 pKa = 3.74VEE234 pKa = 4.4QKK236 pKa = 10.69EE237 pKa = 4.34DD238 pKa = 3.44EE239 pKa = 4.33TGIILPEE246 pKa = 3.97EE247 pKa = 4.38DD248 pKa = 3.15

Molecular weight: 28.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7F0P1|R7F0P1_9BACI Uncharacterized protein OS=Bacillus sp. CAG:988 OX=1262708 GN=BN822_00145 PE=4 SV=1
MM1 pKa = 7.33IQEE4 pKa = 3.93QVKK7 pKa = 9.18QKK9 pKa = 10.3RR10 pKa = 11.84VKK12 pKa = 10.41RR13 pKa = 11.84LIALVMSLFAYY24 pKa = 10.27AFILKK29 pKa = 9.87VMTWLFRR36 pKa = 11.84SFQIRR41 pKa = 11.84NFGYY45 pKa = 10.63CILATLIIYY54 pKa = 8.19GLNVTVKK61 pKa = 10.07PVLFQITLPLTGISFGLFYY80 pKa = 10.65FVLNVFVLKK89 pKa = 9.68LTDD92 pKa = 4.25WILGPAFTVANIWVVFFIAILITICFQVFQSMILKK127 pKa = 9.75PIVRR131 pKa = 11.84RR132 pKa = 11.84IRR134 pKa = 11.84IHH136 pKa = 5.23EE137 pKa = 3.95

Molecular weight: 16.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1397

0

1397

420405

30

2664

300.9

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.25 ± 0.053

1.107 ± 0.026

6.093 ± 0.056

7.43 ± 0.073

4.487 ± 0.056

5.486 ± 0.063

1.807 ± 0.03

8.224 ± 0.067

7.775 ± 0.091

9.382 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.608 ± 0.032

5.422 ± 0.058

3.107 ± 0.04

3.347 ± 0.047

3.736 ± 0.047

6.516 ± 0.063

5.975 ± 0.071

6.624 ± 0.049

0.705 ± 0.022

4.918 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski