Amino acid dipepetide frequency for Bifidobacterium minimum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.933AlaAla: 11.933 ± 0.194	1.054AlaCys: 1.054 ± 0.052	7.149AlaAsp: 7.149 ± 0.124	4.94AlaGlu: 4.94 ± 0.106	3.14AlaPhe: 3.14 ± 0.091	9.042AlaGly: 9.042 ± 0.144	2.174AlaHis: 2.174 ± 0.065	5.587AlaIle: 5.587 ± 0.114	3.237AlaLys: 3.237 ± 0.081	9.974AlaLeu: 9.974 ± 0.154	3.144AlaMet: 3.144 ± 0.071	2.456AlaAsn: 2.456 ± 0.072	3.897AlaPro: 3.897 ± 0.099	3.585AlaGln: 3.585 ± 0.084	7.736AlaArg: 7.736 ± 0.137	7.776AlaSer: 7.776 ± 0.137	6.113AlaThr: 6.113 ± 0.107	9.283AlaVal: 9.283 ± 0.158	1.305AlaTrp: 1.305 ± 0.053	2.343AlaTyr: 2.343 ± 0.063	0.0AlaXaa: 0.0 ± 0.001
Cys	1.041CysAla: 1.041 ± 0.053	0.117CysCys: 0.117 ± 0.016	0.631CysAsp: 0.631 ± 0.037	0.412CysGlu: 0.412 ± 0.029	0.255CysPhe: 0.255 ± 0.018	1.065CysGly: 1.065 ± 0.05	0.233CysHis: 0.233 ± 0.021	0.323CysIle: 0.323 ± 0.024	0.139CysLys: 0.139 ± 0.017	0.79CysLeu: 0.79 ± 0.042	0.22CysMet: 0.22 ± 0.02	0.161CysAsn: 0.161 ± 0.016	0.513CysPro: 0.513 ± 0.031	0.189CysGln: 0.189 ± 0.018	0.643CysArg: 0.643 ± 0.034	0.645CysSer: 0.645 ± 0.04	0.447CysThr: 0.447 ± 0.031	0.777CysVal: 0.777 ± 0.042	0.104CysTrp: 0.104 ± 0.012	0.174CysTyr: 0.174 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	8.21AspAla: 8.21 ± 0.161	0.513AspCys: 0.513 ± 0.028	6.049AspAsp: 6.049 ± 0.126	4.696AspGlu: 4.696 ± 0.099	2.084AspPhe: 2.084 ± 0.071	7.039AspGly: 7.039 ± 0.14	1.743AspHis: 1.743 ± 0.069	3.16AspIle: 3.16 ± 0.08	1.752AspLys: 1.752 ± 0.071	5.552AspLeu: 5.552 ± 0.113	1.796AspMet: 1.796 ± 0.064	1.421AspAsn: 1.421 ± 0.057	4.194AspPro: 4.194 ± 0.097	1.751AspGln: 1.751 ± 0.058	4.55AspArg: 4.55 ± 0.122	4.471AspSer: 4.471 ± 0.092	3.083AspThr: 3.083 ± 0.092	6.08AspVal: 6.08 ± 0.109	0.874AspTrp: 0.874 ± 0.044	1.78AspTyr: 1.78 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	5.292GluAla: 5.292 ± 0.123	0.444GluCys: 0.444 ± 0.031	3.419GluAsp: 3.419 ± 0.1	2.799GluGlu: 2.799 ± 0.083	1.622GluPhe: 1.622 ± 0.049	4.071GluGly: 4.071 ± 0.095	1.301GluHis: 1.301 ± 0.043	2.482GluIle: 2.482 ± 0.07	1.782GluLys: 1.782 ± 0.071	4.462GluLeu: 4.462 ± 0.098	1.349GluMet: 1.349 ± 0.052	1.582GluAsn: 1.582 ± 0.059	2.344GluPro: 2.344 ± 0.073	1.692GluGln: 1.692 ± 0.065	4.442GluArg: 4.442 ± 0.11	3.888GluSer: 3.888 ± 0.091	2.724GluThr: 2.724 ± 0.071	3.479GluVal: 3.479 ± 0.101	0.609GluTrp: 0.609 ± 0.035	1.274GluTyr: 1.274 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.36PheAla: 3.36 ± 0.093	0.277PheCys: 0.277 ± 0.022	2.291PheAsp: 2.291 ± 0.065	1.556PheGlu: 1.556 ± 0.055	1.016PhePhe: 1.016 ± 0.045	2.828PheGly: 2.828 ± 0.076	0.753PheHis: 0.753 ± 0.032	1.518PheIle: 1.518 ± 0.059	0.647PheLys: 0.647 ± 0.039	2.779PheLeu: 2.779 ± 0.075	0.746PheMet: 0.746 ± 0.039	0.922PheAsn: 0.922 ± 0.037	1.411PhePro: 1.411 ± 0.055	0.731PheGln: 0.731 ± 0.04	1.778PheArg: 1.778 ± 0.05	2.286PheSer: 2.286 ± 0.074	1.994PheThr: 1.994 ± 0.068	2.482PheVal: 2.482 ± 0.068	0.348PheTrp: 0.348 ± 0.027	0.741PheTyr: 0.741 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	7.598GlyAla: 7.598 ± 0.146	0.858GlyCys: 0.858 ± 0.046	5.475GlyAsp: 5.475 ± 0.121	4.238GlyGlu: 4.238 ± 0.109	3.135GlyPhe: 3.135 ± 0.064	6.507GlyGly: 6.507 ± 0.13	2.06GlyHis: 2.06 ± 0.064	4.804GlyIle: 4.804 ± 0.108	3.05GlyLys: 3.05 ± 0.091	7.448GlyLeu: 7.448 ± 0.134	2.46GlyMet: 2.46 ± 0.079	2.359GlyAsn: 2.359 ± 0.064	3.113GlyPro: 3.113 ± 0.083	2.363GlyGln: 2.363 ± 0.068	6.471GlyArg: 6.471 ± 0.117	6.705GlySer: 6.705 ± 0.126	5.485GlyThr: 5.485 ± 0.116	7.123GlyVal: 7.123 ± 0.116	1.224GlyTrp: 1.224 ± 0.046	2.44GlyTyr: 2.44 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	2.443HisAla: 2.443 ± 0.066	0.198HisCys: 0.198 ± 0.019	2.051HisAsp: 2.051 ± 0.061	1.234HisGlu: 1.234 ± 0.05	0.499HisPhe: 0.499 ± 0.029	2.251HisGly: 2.251 ± 0.066	0.621HisHis: 0.621 ± 0.041	1.019HisIle: 1.019 ± 0.05	0.445HisLys: 0.445 ± 0.027	1.653HisLeu: 1.653 ± 0.051	0.566HisMet: 0.566 ± 0.029	0.563HisAsn: 0.563 ± 0.033	1.575HisPro: 1.575 ± 0.058	0.62HisGln: 0.62 ± 0.034	1.879HisArg: 1.879 ± 0.062	1.157HisSer: 1.157 ± 0.045	1.104HisThr: 1.104 ± 0.042	1.971HisVal: 1.971 ± 0.059	0.312HisTrp: 0.312 ± 0.026	0.563HisTyr: 0.563 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.084IleAla: 6.084 ± 0.113	0.455IleCys: 0.455 ± 0.028	4.135IleAsp: 4.135 ± 0.098	2.755IleGlu: 2.755 ± 0.073	1.21IlePhe: 1.21 ± 0.051	4.396IleGly: 4.396 ± 0.091	1.144IleHis: 1.144 ± 0.044	2.953IleIle: 2.953 ± 0.091	1.206IleLys: 1.206 ± 0.056	4.071IleLeu: 4.071 ± 0.099	1.21IleMet: 1.21 ± 0.045	1.411IleAsn: 1.411 ± 0.052	2.935IlePro: 2.935 ± 0.073	1.158IleGln: 1.158 ± 0.047	3.556IleArg: 3.556 ± 0.09	3.136IleSer: 3.136 ± 0.083	3.215IleThr: 3.215 ± 0.081	5.006IleVal: 5.006 ± 0.118	0.554IleTrp: 0.554 ± 0.036	0.931IleTyr: 0.931 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	3.78LysAla: 3.78 ± 0.108	0.115LysCys: 0.115 ± 0.013	2.033LysAsp: 2.033 ± 0.06	1.52LysGlu: 1.52 ± 0.058	0.636LysPhe: 0.636 ± 0.043	2.399LysGly: 2.399 ± 0.074	0.475LysHis: 0.475 ± 0.026	1.309LysIle: 1.309 ± 0.065	1.146LysLys: 1.146 ± 0.058	2.148LysLeu: 2.148 ± 0.08	0.532LysMet: 0.532 ± 0.029	0.948LysAsn: 0.948 ± 0.048	1.481LysPro: 1.481 ± 0.058	0.874LysGln: 0.874 ± 0.05	1.837LysArg: 1.837 ± 0.061	1.923LysSer: 1.923 ± 0.076	1.939LysThr: 1.939 ± 0.063	2.132LysVal: 2.132 ± 0.077	0.282LysTrp: 0.282 ± 0.024	0.719LysTyr: 0.719 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	9.565LeuAla: 9.565 ± 0.164	0.909LeuCys: 0.909 ± 0.045	6.333LeuAsp: 6.333 ± 0.111	3.947LeuGlu: 3.947 ± 0.106	2.707LeuPhe: 2.707 ± 0.071	7.035LeuGly: 7.035 ± 0.118	1.905LeuHis: 1.905 ± 0.066	4.592LeuIle: 4.592 ± 0.128	2.381LeuLys: 2.381 ± 0.075	7.528LeuLeu: 7.528 ± 0.15	2.295LeuMet: 2.295 ± 0.07	2.224LeuAsn: 2.224 ± 0.062	4.372LeuPro: 4.372 ± 0.089	1.923LeuGln: 1.923 ± 0.064	6.451LeuArg: 6.451 ± 0.121	6.863LeuSer: 6.863 ± 0.124	5.514LeuThr: 5.514 ± 0.112	7.054LeuVal: 7.054 ± 0.14	1.056LeuTrp: 1.056 ± 0.048	1.785LeuTyr: 1.785 ± 0.069	0.002LeuXaa: 0.002 ± 0.001
Met	3.146MetAla: 3.146 ± 0.081	0.203MetCys: 0.203 ± 0.02	1.743MetAsp: 1.743 ± 0.057	1.215MetGlu: 1.215 ± 0.053	0.733MetPhe: 0.733 ± 0.041	2.18MetGly: 2.18 ± 0.064	0.515MetHis: 0.515 ± 0.029	1.303MetIle: 1.303 ± 0.049	0.742MetLys: 0.742 ± 0.043	2.332MetLeu: 2.332 ± 0.066	0.797MetMet: 0.797 ± 0.043	0.779MetAsn: 0.779 ± 0.036	1.461MetPro: 1.461 ± 0.057	0.598MetGln: 0.598 ± 0.035	2.176MetArg: 2.176 ± 0.061	2.22MetSer: 2.22 ± 0.067	2.141MetThr: 2.141 ± 0.066	2.137MetVal: 2.137 ± 0.069	0.288MetTrp: 0.288 ± 0.024	0.471MetTyr: 0.471 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.781AsnAla: 2.781 ± 0.075	0.189AsnCys: 0.189 ± 0.019	1.774AsnAsp: 1.774 ± 0.067	1.268AsnGlu: 1.268 ± 0.044	0.693AsnPhe: 0.693 ± 0.042	2.711AsnGly: 2.711 ± 0.088	0.57AsnHis: 0.57 ± 0.037	1.243AsnIle: 1.243 ± 0.053	0.73AsnLys: 0.73 ± 0.046	2.136AsnLeu: 2.136 ± 0.068	0.649AsnMet: 0.649 ± 0.036	0.746AsnAsn: 0.746 ± 0.051	1.938AsnPro: 1.938 ± 0.062	0.722AsnGln: 0.722 ± 0.043	1.811AsnArg: 1.811 ± 0.053	1.516AsnSer: 1.516 ± 0.064	1.52AsnThr: 1.52 ± 0.057	2.055AsnVal: 2.055 ± 0.061	0.315AsnTrp: 0.315 ± 0.028	0.735AsnTyr: 0.735 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	4.594ProAla: 4.594 ± 0.096	0.379ProCys: 0.379 ± 0.027	3.926ProAsp: 3.926 ± 0.091	2.99ProGlu: 2.99 ± 0.077	1.521ProPhe: 1.521 ± 0.054	3.965ProGly: 3.965 ± 0.094	1.169ProHis: 1.169 ± 0.046	2.106ProIle: 2.106 ± 0.064	1.202ProLys: 1.202 ± 0.054	3.901ProLeu: 3.901 ± 0.084	1.191ProMet: 1.191 ± 0.046	1.144ProAsn: 1.144 ± 0.044	1.545ProPro: 1.545 ± 0.069	1.73ProGln: 1.73 ± 0.058	3.58ProArg: 3.58 ± 0.104	3.824ProSer: 3.824 ± 0.083	3.125ProThr: 3.125 ± 0.091	4.205ProVal: 4.205 ± 0.092	0.719ProTrp: 0.719 ± 0.034	1.234ProTyr: 1.234 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	3.105GlnAla: 3.105 ± 0.085	0.231GlnCys: 0.231 ± 0.019	1.521GlnAsp: 1.521 ± 0.048	1.567GlnGlu: 1.567 ± 0.054	0.808GlnPhe: 0.808 ± 0.039	2.39GlnGly: 2.39 ± 0.076	0.546GlnHis: 0.546 ± 0.035	1.707GlnIle: 1.707 ± 0.056	0.929GlnLys: 0.929 ± 0.049	2.531GlnLeu: 2.531 ± 0.075	0.821GlnMet: 0.821 ± 0.038	0.821GlnAsn: 0.821 ± 0.046	1.129GlnPro: 1.129 ± 0.051	1.074GlnGln: 1.074 ± 0.054	2.311GlnArg: 2.311 ± 0.075	1.938GlnSer: 1.938 ± 0.066	1.716GlnThr: 1.716 ± 0.054	2.073GlnVal: 2.073 ± 0.06	0.539GlnTrp: 0.539 ± 0.032	0.753GlnTyr: 0.753 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	6.322ArgAla: 6.322 ± 0.128	0.64ArgCys: 0.64 ± 0.045	4.936ArgAsp: 4.936 ± 0.12	4.106ArgGlu: 4.106 ± 0.11	2.552ArgPhe: 2.552 ± 0.072	5.16ArgGly: 5.16 ± 0.111	2.141ArgHis: 2.141 ± 0.071	4.333ArgIle: 4.333 ± 0.097	2.178ArgLys: 2.178 ± 0.064	6.713ArgLeu: 6.713 ± 0.156	2.429ArgMet: 2.429 ± 0.068	1.985ArgAsn: 1.985 ± 0.062	3.49ArgPro: 3.49 ± 0.082	2.381ArgGln: 2.381 ± 0.074	7.409ArgArg: 7.409 ± 0.187	5.127ArgSer: 5.127 ± 0.121	4.146ArgThr: 4.146 ± 0.092	5.36ArgVal: 5.36 ± 0.101	1.094ArgTrp: 1.094 ± 0.048	2.108ArgTyr: 2.108 ± 0.063	0.0ArgXaa: 0.0 ± 0.0
Ser	7.481SerAla: 7.481 ± 0.123	0.631SerCys: 0.631 ± 0.037	4.9SerAsp: 4.9 ± 0.108	3.025SerGlu: 3.025 ± 0.08	2.051SerPhe: 2.051 ± 0.063	6.859SerGly: 6.859 ± 0.129	1.622SerHis: 1.622 ± 0.054	3.556SerIle: 3.556 ± 0.086	1.785SerLys: 1.785 ± 0.055	6.249SerLeu: 6.249 ± 0.106	2.044SerMet: 2.044 ± 0.065	1.752SerAsn: 1.752 ± 0.069	3.338SerPro: 3.338 ± 0.091	2.398SerGln: 2.398 ± 0.077	5.499SerArg: 5.499 ± 0.114	6.856SerSer: 6.856 ± 0.23	4.834SerThr: 4.834 ± 0.128	5.664SerVal: 5.664 ± 0.112	0.972SerTrp: 0.972 ± 0.046	1.705SerTyr: 1.705 ± 0.066	0.0SerXaa: 0.0 ± 0.0
Thr	6.419ThrAla: 6.419 ± 0.122	0.466ThrCys: 0.466 ± 0.03	3.734ThrAsp: 3.734 ± 0.091	2.489ThrGlu: 2.489 ± 0.079	1.773ThrPhe: 1.773 ± 0.064	5.474ThrGly: 5.474 ± 0.116	1.283ThrHis: 1.283 ± 0.056	3.461ThrIle: 3.461 ± 0.086	1.602ThrLys: 1.602 ± 0.058	5.21ThrLeu: 5.21 ± 0.102	1.672ThrMet: 1.672 ± 0.061	1.633ThrAsn: 1.633 ± 0.075	3.521ThrPro: 3.521 ± 0.088	1.648ThrGln: 1.648 ± 0.06	3.925ThrArg: 3.925 ± 0.1	4.236ThrSer: 4.236 ± 0.105	4.52ThrThr: 4.52 ± 0.127	5.756ThrVal: 5.756 ± 0.112	0.753ThrTrp: 0.753 ± 0.039	1.417ThrTyr: 1.417 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	9.028ValAla: 9.028 ± 0.155	0.841ValCys: 0.841 ± 0.037	6.181ValAsp: 6.181 ± 0.124	4.449ValGlu: 4.449 ± 0.1	2.751ValPhe: 2.751 ± 0.082	6.135ValGly: 6.135 ± 0.14	1.659ValHis: 1.659 ± 0.058	4.449ValIle: 4.449 ± 0.087	2.246ValLys: 2.246 ± 0.073	7.567ValLeu: 7.567 ± 0.147	2.247ValMet: 2.247 ± 0.074	2.169ValAsn: 2.169 ± 0.063	4.073ValPro: 4.073 ± 0.085	1.855ValGln: 1.855 ± 0.063	5.56ValArg: 5.56 ± 0.11	6.19ValSer: 6.19 ± 0.103	5.186ValThr: 5.186 ± 0.101	8.177ValVal: 8.177 ± 0.165	1.006ValTrp: 1.006 ± 0.045	1.73ValTyr: 1.73 ± 0.066	0.002ValXaa: 0.002 ± 0.002
Trp	1.061TrpAla: 1.061 ± 0.047	0.134TrpCys: 0.134 ± 0.014	0.702TrpAsp: 0.702 ± 0.038	0.5TrpGlu: 0.5 ± 0.037	0.493TrpPhe: 0.493 ± 0.033	1.001TrpGly: 1.001 ± 0.045	0.29TrpHis: 0.29 ± 0.026	0.693TrpIle: 0.693 ± 0.033	0.444TrpLys: 0.444 ± 0.031	1.323TrpLeu: 1.323 ± 0.052	0.502TrpMet: 0.502 ± 0.033	0.455TrpAsn: 0.455 ± 0.033	0.546TrpPro: 0.546 ± 0.03	0.438TrpGln: 0.438 ± 0.032	1.104TrpArg: 1.104 ± 0.047	0.999TrpSer: 0.999 ± 0.042	0.891TrpThr: 0.891 ± 0.045	0.761TrpVal: 0.761 ± 0.036	0.28TrpTrp: 0.28 ± 0.024	0.37TrpTyr: 0.37 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.623TyrAla: 2.623 ± 0.066	0.227TyrCys: 0.227 ± 0.023	1.815TyrAsp: 1.815 ± 0.062	1.349TyrGlu: 1.349 ± 0.056	0.788TyrPhe: 0.788 ± 0.044	2.407TyrGly: 2.407 ± 0.082	0.517TyrHis: 0.517 ± 0.03	0.924TyrIle: 0.924 ± 0.042	0.583TyrLys: 0.583 ± 0.041	2.073TyrLeu: 2.073 ± 0.059	0.508TyrMet: 0.508 ± 0.031	0.57TyrAsn: 0.57 ± 0.043	1.175TyrPro: 1.175 ± 0.047	0.783TyrGln: 0.783 ± 0.037	1.895TyrArg: 1.895 ± 0.065	1.465TyrSer: 1.465 ± 0.05	1.292TyrThr: 1.292 ± 0.064	1.927TyrVal: 1.927 ± 0.062	0.352TyrTrp: 0.352 ± 0.026	0.665TyrTyr: 0.665 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1589 proteins (545536 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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