Mycobacterium phage Newman

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; Mycobacterium virus Suffolk

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TAN8|R4TAN8_9CAUD Uncharacterized protein OS=Mycobacterium phage Newman OX=1327766 GN=67 PE=4 SV=1
MM1 pKa = 6.88FTDD4 pKa = 3.48PRR6 pKa = 11.84IRR8 pKa = 11.84SAFLAGVAAGAALGVTVGAALVLTALPDD36 pKa = 3.92DD37 pKa = 4.34VSVPRR42 pKa = 11.84PGLSVTEE49 pKa = 4.16TAATKK54 pKa = 10.15QFAMWPADD62 pKa = 3.56PAPTPEE68 pKa = 5.55DD69 pKa = 4.42DD70 pKa = 3.69PAFDD74 pKa = 4.19CRR76 pKa = 11.84YY77 pKa = 9.45HH78 pKa = 6.82GNHH81 pKa = 5.92ICGPLNVDD89 pKa = 4.11GYY91 pKa = 11.9APGLYY96 pKa = 9.09IDD98 pKa = 4.99GVLVDD103 pKa = 4.79PWPVDD108 pKa = 3.43PASTPTIEE116 pKa = 4.27VPP118 pKa = 3.66

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4TRS2|R4TRS2_9CAUD Uncharacterized protein OS=Mycobacterium phage Newman OX=1327766 GN=71 PE=4 SV=1
MM1 pKa = 7.7CPPRR5 pKa = 11.84YY6 pKa = 9.56ILIYY10 pKa = 8.99MVNPKK15 pKa = 9.91RR16 pKa = 11.84PHH18 pKa = 6.48RR19 pKa = 11.84AFQQTPAKK27 pKa = 9.24PATILLPIKK36 pKa = 10.03CPPDD40 pKa = 3.02VRR42 pKa = 11.84QRR44 pKa = 11.84LKK46 pKa = 10.42IAAAEE51 pKa = 4.46DD52 pKa = 3.82GLTYY56 pKa = 10.82AQLLVSLLDD65 pKa = 3.85LRR67 pKa = 11.84DD68 pKa = 3.63DD69 pKa = 3.33RR70 pKa = 11.84HH71 pKa = 5.51RR72 pKa = 11.84RR73 pKa = 11.84QRR75 pKa = 11.84AAQASPLHH83 pKa = 6.43RR84 pKa = 11.84PRR86 pKa = 11.84AEE88 pKa = 3.93TAA90 pKa = 3.09

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

21499

38

1992

221.6

23.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.168 ± 0.342

1.074 ± 0.124

6.572 ± 0.257

5.875 ± 0.347

2.302 ± 0.097

9.642 ± 0.65

2.051 ± 0.175

4.516 ± 0.156

2.921 ± 0.156

8.047 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.051 ± 0.107

2.861 ± 0.211

6.121 ± 0.286

3.089 ± 0.122

6.865 ± 0.354

4.903 ± 0.209

7.0 ± 0.169

7.568 ± 0.173

1.865 ± 0.142

2.507 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski