Mulberry vein banding virus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5H8S9|A0A0C5H8S9_9VIRU NSs OS=Mulberry vein banding virus OX=1266451 GN=NSs PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.1 KK3 pKa = 10.38 YY4 pKa = 10.43 YY5 pKa = 10.4 LPSYY9 pKa = 10.57 CLGLVLLFLVSEE21 pKa = 4.82 VYY23 pKa = 10.75 LINQMEE29 pKa = 4.77 HH30 pKa = 6.7 DD31 pKa = 4.13 VQVKK35 pKa = 9.64 RR36 pKa = 11.84 FQSRR40 pKa = 11.84 YY41 pKa = 9.7 KK42 pKa = 10.84 ADD44 pKa = 4.28 DD45 pKa = 4.21 PDD47 pKa = 4.95 DD48 pKa = 3.86 LVEE51 pKa = 4.99 IEE53 pKa = 5.14 EE54 pKa = 4.24 PTEE57 pKa = 4.46 SIVHH61 pKa = 5.02 EE62 pKa = 4.16 TRR64 pKa = 11.84 FQKK67 pKa = 10.34 KK68 pKa = 9.38 KK69 pKa = 9.36 KK70 pKa = 9.79 LSRR73 pKa = 11.84 ILRR76 pKa = 11.84 DD77 pKa = 4.03 DD78 pKa = 3.63 EE79 pKa = 4.44 ATTQSSASKK88 pKa = 10.31 LDD90 pKa = 3.3 CDD92 pKa = 3.78 KK93 pKa = 11.35 FEE95 pKa = 5.52 KK96 pKa = 10.22 RR97 pKa = 11.84 YY98 pKa = 10.3 CLIKK102 pKa = 10.68 GVSDD106 pKa = 4.67 FNAHH110 pKa = 5.16 YY111 pKa = 10.54 QLDD114 pKa = 3.85 NGEE117 pKa = 4.69 EE118 pKa = 4.11 IISCISNSASIFEE131 pKa = 4.18 IYY133 pKa = 10.16 QYY135 pKa = 11.12 EE136 pKa = 4.28 KK137 pKa = 10.36 EE138 pKa = 4.09 FKK140 pKa = 10.25 KK141 pKa = 10.84 IKK143 pKa = 10.5 FKK145 pKa = 11.29 DD146 pKa = 3.72 FLVVPVLKK154 pKa = 10.98 LEE156 pKa = 4.19 NKK158 pKa = 9.6 KK159 pKa = 10.37 VLEE162 pKa = 4.28 VGTKK166 pKa = 9.75 FFFVDD171 pKa = 3.86 KK172 pKa = 11.02 SNNPVNIDD180 pKa = 3.23 PKK182 pKa = 11.28 VNLKK186 pKa = 10.65 SPTVSKK192 pKa = 10.95 LSVRR196 pKa = 11.84 LSGDD200 pKa = 3.35 CKK202 pKa = 10.31 INQVSMSTPYY212 pKa = 10.12 QIKK215 pKa = 10.18 LRR217 pKa = 11.84 SEE219 pKa = 4.07 EE220 pKa = 4.14 NIGVLVKK227 pKa = 10.53 NVKK230 pKa = 9.88 DD231 pKa = 3.74 PKK233 pKa = 10.28 SGNVKK238 pKa = 9.78 TIFGDD243 pKa = 3.53 STINFQPEE251 pKa = 4.21 EE252 pKa = 3.9 LDD254 pKa = 3.63 GNHH257 pKa = 6.69 FLLCGDD263 pKa = 3.78 KK264 pKa = 10.89 SSLIMKK270 pKa = 9.73 VDD272 pKa = 3.41 VAVRR276 pKa = 11.84 NCVSKK281 pKa = 11.45 YY282 pKa = 9.76 SDD284 pKa = 3.57 EE285 pKa = 4.08 PKK287 pKa = 10.64 KK288 pKa = 10.85 IFFCTNFSYY297 pKa = 10.01 FKK299 pKa = 9.94 WIFVFLMVAFPISWLVWKK317 pKa = 8.41 TKK319 pKa = 9.99 NALSIWYY326 pKa = 8.68 DD327 pKa = 2.89 IVGIVTYY334 pKa = 9.66 PILWILNWFWPYY346 pKa = 10.73 FPFKK350 pKa = 10.72 CRR352 pKa = 11.84 ICGCISFLTHH362 pKa = 6.29 TCTEE366 pKa = 4.1 KK367 pKa = 10.85 CVCNQSEE374 pKa = 4.06 ASKK377 pKa = 11.09 DD378 pKa = 3.68 HH379 pKa = 6.31 TSEE382 pKa = 4.64 CYY384 pKa = 10.31 LFSRR388 pKa = 11.84 DD389 pKa = 3.45 KK390 pKa = 11.21 KK391 pKa = 10.74 DD392 pKa = 3.35 WNEE395 pKa = 3.79 LTLLQQFQFTINTKK409 pKa = 9.64 ISTNFLVFVTKK420 pKa = 10.06 MIIASILISYY430 pKa = 9.28 IPSSIALQQSNLCVEE445 pKa = 4.06 KK446 pKa = 10.54 CYY448 pKa = 11.35 YY449 pKa = 10.34 NLNLDD454 pKa = 3.69 SLTTDD459 pKa = 3.39 RR460 pKa = 11.84 FGLSKK465 pKa = 11.33 NNFEE469 pKa = 4.33 TCEE472 pKa = 3.98 CSIGNVITEE481 pKa = 4.44 TIYY484 pKa = 11.04 RR485 pKa = 11.84 EE486 pKa = 4.45 GIPVSRR492 pKa = 11.84 ATSLNDD498 pKa = 4.04 CIVGSDD504 pKa = 3.24 TCMVSDD510 pKa = 4.39 NQAQNLFACRR520 pKa = 11.84 NGCNSLNSIKK530 pKa = 10.59 NIPDD534 pKa = 3.3 TKK536 pKa = 10.39 FSKK539 pKa = 10.47 FYY541 pKa = 9.96 KK542 pKa = 10.08 GKK544 pKa = 10.02 SFRR547 pKa = 11.84 GNLTSLKK554 pKa = 9.48 IANRR558 pKa = 11.84 LRR560 pKa = 11.84 EE561 pKa = 4.28 GYY563 pKa = 8.76 MDD565 pKa = 4.73 SPTEE569 pKa = 4.08 SKK571 pKa = 10.44 ILEE574 pKa = 4.26 EE575 pKa = 4.12 EE576 pKa = 3.94 STRR579 pKa = 11.84 EE580 pKa = 3.72 YY581 pKa = 11.24 KK582 pKa = 10.42 FYY584 pKa = 11.36 SSLKK588 pKa = 10.0 VDD590 pKa = 5.69 DD591 pKa = 4.95 IPPEE595 pKa = 3.94 NLMPRR600 pKa = 11.84 QSLVFSTEE608 pKa = 3.11 VDD610 pKa = 3.09 GKK612 pKa = 10.74 YY613 pKa = 9.97 RR614 pKa = 11.84 YY615 pKa = 9.71 LLEE618 pKa = 4.11 MDD620 pKa = 4.09 IKK622 pKa = 11.06 ASTGSVYY629 pKa = 10.98 LLNDD633 pKa = 4.31 DD634 pKa = 4.88 ASHH637 pKa = 7.11 SPMEE641 pKa = 3.71 FMVYY645 pKa = 9.25 VKK647 pKa = 10.53 SVGVEE652 pKa = 3.2 YY653 pKa = 10.4 DD654 pKa = 2.72 IRR656 pKa = 11.84 YY657 pKa = 9.56 KK658 pKa = 11.11 YY659 pKa = 9.59 STAKK663 pKa = 10.07 IDD665 pKa = 3.86 TTVSDD670 pKa = 4.23 YY671 pKa = 11.57 LVTCTGKK678 pKa = 10.63 CADD681 pKa = 5.18 CIKK684 pKa = 10.37 QKK686 pKa = 10.68 PKK688 pKa = 10.93 VGVLDD693 pKa = 4.06 FCVTPTSWWGCEE705 pKa = 3.82 EE706 pKa = 4.53 LGCLAINEE714 pKa = 4.33 GAICGHH720 pKa = 6.11 CTNIYY725 pKa = 10.33 DD726 pKa = 4.66 LSSLINIYY734 pKa = 8.6 QVIEE738 pKa = 4.08 SHH740 pKa = 5.38 VTAEE744 pKa = 4.13 ICVKK748 pKa = 10.77 SLDD751 pKa = 3.93 GYY753 pKa = 9.1 SCKK756 pKa = 10.14 KK757 pKa = 10.17 HH758 pKa = 6.52 SDD760 pKa = 3.26 RR761 pKa = 11.84 SPIQTDD767 pKa = 3.94 HH768 pKa = 6.5 YY769 pKa = 9.97 QLDD772 pKa = 3.87 MSIDD776 pKa = 3.46 LHH778 pKa = 7.51 NDD780 pKa = 2.92 YY781 pKa = 10.78 MSTDD785 pKa = 3.08 KK786 pKa = 11.27 LFAVNKK792 pKa = 6.17 QQKK795 pKa = 9.51 VLTGNIADD803 pKa = 4.67 LGDD806 pKa = 3.62 FAGSSFGHH814 pKa = 5.78 PQITVDD820 pKa = 4.2 GVPLAVPAALTQNDD834 pKa = 4.24 FSWSCSAVGEE844 pKa = 4.14 KK845 pKa = 10.15 KK846 pKa = 10.59 INIRR850 pKa = 11.84 QCGLYY855 pKa = 8.68 TYY857 pKa = 11.35 SMVYY861 pKa = 10.1 VLSPSKK867 pKa = 10.88 DD868 pKa = 3.33 NSHH871 pKa = 6.49 LQEE874 pKa = 5.2 DD875 pKa = 4.13 KK876 pKa = 11.39 NKK878 pKa = 10.92 LYY880 pKa = 9.99 MEE882 pKa = 4.78 KK883 pKa = 10.73 DD884 pKa = 3.46 FLVGKK889 pKa = 9.98 LKK891 pKa = 10.25 MVIDD895 pKa = 3.96 MPKK898 pKa = 10.62 EE899 pKa = 3.83 MFKK902 pKa = 10.59 KK903 pKa = 10.72 VPTKK907 pKa = 10.34 PVLSEE912 pKa = 3.96 TKK914 pKa = 7.94 MTCSGCTQCAVGIDD928 pKa = 3.83 CNITYY933 pKa = 9.74 TSDD936 pKa = 3.04 TTFSSRR942 pKa = 11.84 IMMDD946 pKa = 2.76 SCSFKK951 pKa = 10.68 SDD953 pKa = 3.31 QLGTFLGPNEE963 pKa = 4.17 KK964 pKa = 9.49 TIKK967 pKa = 10.0 AYY969 pKa = 10.5 CSEE972 pKa = 4.54 SIVDD976 pKa = 3.9 KK977 pKa = 10.63 SLKK980 pKa = 10.17 FIPEE984 pKa = 4.16 DD985 pKa = 3.94 QEE987 pKa = 4.81 DD988 pKa = 4.31 LTVDD992 pKa = 3.24 IQVDD996 pKa = 3.66 EE997 pKa = 4.77 FVQVDD1002 pKa = 3.77 QDD1004 pKa = 4.31 TIIHH1008 pKa = 6.59 FDD1010 pKa = 3.87 DD1011 pKa = 5.37 KK1012 pKa = 11.3 SAHH1015 pKa = 6.98 DD1016 pKa = 4.17 EE1017 pKa = 4.3 NKK1019 pKa = 9.65 HH1020 pKa = 6.34 HH1021 pKa = 7.3 SDD1023 pKa = 3.33 TSISSLWDD1031 pKa = 3.04 WVKK1034 pKa = 11.29 APFNWVASFFGSFFDD1049 pKa = 4.86 LIRR1052 pKa = 11.84 IILVVLAICVGIYY1065 pKa = 10.04 ILNCIFKK1072 pKa = 10.54 LSKK1075 pKa = 9.55 TYY1077 pKa = 10.93 YY1078 pKa = 8.39 IDD1080 pKa = 3.39 KK1081 pKa = 10.01 RR1082 pKa = 11.84 RR1083 pKa = 11.84 QRR1085 pKa = 11.84 MEE1087 pKa = 4.01 DD1088 pKa = 3.04 AVEE1091 pKa = 4.48 SIEE1094 pKa = 5.12 SSVLLTNYY1102 pKa = 9.12 TGLDD1106 pKa = 3.16 QTRR1109 pKa = 11.84 KK1110 pKa = 9.82 RR1111 pKa = 11.84 KK1112 pKa = 9.84 SPPKK1116 pKa = 9.65 GYY1118 pKa = 10.27 EE1119 pKa = 3.6 FSLEE1123 pKa = 4.06 VV1124 pKa = 3.32
Molecular weight: 128.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.576
IPC2_protein 5.715
IPC_protein 5.741
Toseland 5.893
ProMoST 6.071
Dawson 5.97
Bjellqvist 6.008
Wikipedia 5.957
Rodwell 5.944
Grimsley 5.931
Solomon 5.97
Lehninger 5.957
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.376
EMBOSS 6.338
Sillero 6.326
Patrickios 3.795
IPC_peptide 5.982
IPC2_peptide 6.262
IPC2.peptide.svr19 6.348
Protein with the highest isoelectric point:
>tr|K9UTA1|K9UTA1_9VIRU Nucleoprotein OS=Mulberry vein banding virus OX=1266451 GN=N PE=3 SV=1
MM1 pKa = 6.79 STVRR5 pKa = 11.84 QLSEE9 pKa = 4.2 SKK11 pKa = 8.9 ITALLAGGKK20 pKa = 9.91 ADD22 pKa = 3.89 VEE24 pKa = 4.42 IEE26 pKa = 4.37 TEE28 pKa = 3.97 EE29 pKa = 4.13 STPGFNFKK37 pKa = 11.0 AFFDD41 pKa = 4.0 KK42 pKa = 11.31 NHH44 pKa = 6.51 DD45 pKa = 3.77 VEE47 pKa = 4.81 ITFATCLNILKK58 pKa = 10.14 SRR60 pKa = 11.84 KK61 pKa = 9.54 QIFAACKK68 pKa = 7.51 NGKK71 pKa = 9.75 YY72 pKa = 10.09 NFCGHH77 pKa = 6.77 NIIATTANAGIDD89 pKa = 3.23 DD90 pKa = 3.94 WTFKK94 pKa = 9.99 RR95 pKa = 11.84 TEE97 pKa = 3.48 ALIRR101 pKa = 11.84 VKK103 pKa = 9.93 MIRR106 pKa = 11.84 MVEE109 pKa = 3.92 KK110 pKa = 10.42 AASNEE115 pKa = 4.25 SKK117 pKa = 10.56 LAMYY121 pKa = 9.46 QKK123 pKa = 10.8 VMEE126 pKa = 4.83 LPLVSAYY133 pKa = 10.41 GLNAPPEE140 pKa = 4.26 FNACALRR147 pKa = 11.84 LMLCIGGPLSLLSSFKK163 pKa = 10.84 ALAPIVFPLAYY174 pKa = 8.49 YY175 pKa = 10.63 QNVKK179 pKa = 10.31 KK180 pKa = 10.66 EE181 pKa = 3.8 KK182 pKa = 10.6 LGIKK186 pKa = 10.06 NFSTYY191 pKa = 9.83 EE192 pKa = 3.92 QVCKK196 pKa = 9.96 VAKK199 pKa = 9.93 VLSAAQFDD207 pKa = 4.1 FQGDD211 pKa = 4.14 FKK213 pKa = 10.94 TLFEE217 pKa = 4.59 EE218 pKa = 4.34 AVKK221 pKa = 9.97 MLSEE225 pKa = 4.61 SNPGTASSISLKK237 pKa = 10.73 KK238 pKa = 10.13 YY239 pKa = 10.29 DD240 pKa = 3.9 EE241 pKa = 4.12 QVKK244 pKa = 10.67 YY245 pKa = 8.93 MDD247 pKa = 4.24 KK248 pKa = 10.69 VFSSSLVVDD257 pKa = 4.91 DD258 pKa = 4.72 YY259 pKa = 12.27 GEE261 pKa = 4.01 NSKK264 pKa = 10.83 KK265 pKa = 10.26 KK266 pKa = 9.67 SQPSTSNSIEE276 pKa = 3.93 VV277 pKa = 3.25
Molecular weight: 30.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.52
IPC2_protein 7.849
IPC_protein 7.746
Toseland 7.541
ProMoST 8.287
Dawson 8.536
Bjellqvist 8.726
Wikipedia 8.478
Rodwell 8.595
Grimsley 7.439
Solomon 8.653
Lehninger 8.668
Nozaki 8.96
DTASelect 8.521
Thurlkill 8.624
EMBOSS 8.741
Sillero 8.902
Patrickios 4.52
IPC_peptide 8.653
IPC2_peptide 7.454
IPC2.peptide.svr19 7.828
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5026
277
2877
1005.2
114.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.278 ± 1.055
2.189 ± 0.506
5.969 ± 0.417
6.526 ± 0.395
4.895 ± 0.287
4.019 ± 0.383
1.83 ± 0.26
7.461 ± 0.364
8.894 ± 0.256
9.272 ± 0.575
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.402 ± 0.585
6.327 ± 0.441
2.865 ± 0.284
2.945 ± 0.168
3.243 ± 0.299
9.471 ± 0.384
5.591 ± 0.158
5.949 ± 0.699
0.816 ± 0.174
4.059 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here