Bacillus sp. UFRGS-B20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N8J3I2|A0A2N8J3I2_9BACI Uncharacterized protein OS=Bacillus sp. UFRGS-B20 OX=2070341 GN=CW304_32805 PE=4 SV=1
MM1 pKa = 7.6VDD3 pKa = 4.9LIFEE7 pKa = 4.41SVMDD11 pKa = 4.43LFLLFFKK18 pKa = 10.57MNLSYY23 pKa = 10.5PVISNSPDD31 pKa = 2.56GCMVVEE37 pKa = 4.16YY38 pKa = 10.96GSLLLSLRR46 pKa = 11.84FGFLLTLFGFLLFLACCSFQLNATT70 pKa = 4.0

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N8J6Z4|A0A2N8J6Z4_9BACI Uncharacterized protein OS=Bacillus sp. UFRGS-B20 OX=2070341 GN=CW304_11815 PE=4 SV=1
MM1 pKa = 7.73ISIINALLTVPNRR14 pKa = 11.84KK15 pKa = 8.45MFPPLPNTNKK25 pKa = 8.33ITIRR29 pKa = 11.84KK30 pKa = 6.36TRR32 pKa = 11.84ANAKK36 pKa = 9.78LGSFLSQSNIFASASRR52 pKa = 11.84ASFFVLNPHH61 pKa = 6.91ALLFF65 pKa = 4.53

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1976

0

1976

159495

14

258

80.7

9.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.532 ± 0.063

3.246 ± 0.055

2.472 ± 0.042

2.917 ± 0.052

8.825 ± 0.099

3.562 ± 0.064

3.305 ± 0.046

7.571 ± 0.077

5.523 ± 0.071

11.725 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.036

4.89 ± 0.061

4.595 ± 0.066

3.373 ± 0.043

5.46 ± 0.066

9.223 ± 0.087

5.344 ± 0.062

5.292 ± 0.062

1.214 ± 0.031

4.179 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski