Bacteroidetes bacterium SCGC AAA795-G10
Average proteome isoelectric point is 7.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1767 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2S0H3|A0A2U2S0H3_9BACT Uncharacterized protein OS=Bacteroidetes bacterium SCGC AAA795-G10 OX=2175803 GN=DEJ39_01680 PE=3 SV=1
MM1 pKa = 7.54 KK2 pKa = 9.83 EE3 pKa = 3.98 VKK5 pKa = 10.35 SLLFCLLSIPSFIFGQIVTDD25 pKa = 4.19 GLILNLDD32 pKa = 3.76 TRR34 pKa = 11.84 DD35 pKa = 3.62 SNSYY39 pKa = 10.17 SGSGNTWNDD48 pKa = 2.6 ISGNNYY54 pKa = 9.28 NATIVGGTSFSNNQFTLDD72 pKa = 3.44 GSNNYY77 pKa = 9.34 ISTPAIPSVGISTQDD92 pKa = 3.11 YY93 pKa = 8.36 SLEE96 pKa = 4.31 VIVTPTSTSGNVINMSNQSDD116 pKa = 3.69 NLGWNMPPLASANSTFYY133 pKa = 11.27 AQLHH137 pKa = 5.78 SSANGVTNGISSSFNVNQKK156 pKa = 9.58 YY157 pKa = 10.39 HH158 pKa = 6.91 LVLTWDD164 pKa = 3.51 YY165 pKa = 11.59 DD166 pKa = 3.75 ANANNRR172 pKa = 11.84 VFKK175 pKa = 10.84 LYY177 pKa = 10.91 VNNVLVGQRR186 pKa = 11.84 SNITYY191 pKa = 10.44 SSSGSNNYY199 pKa = 9.1 FFLGKK204 pKa = 9.98 DD205 pKa = 3.33 NPGCCDD211 pKa = 3.05 EE212 pKa = 4.49 FNGVEE217 pKa = 4.09 SRR219 pKa = 11.84 DD220 pKa = 3.56 FAGKK224 pKa = 9.55 YY225 pKa = 7.41 EE226 pKa = 3.98 AFRR229 pKa = 11.84 VYY231 pKa = 10.82 SKK233 pKa = 11.13 ALSAQEE239 pKa = 3.8 VAQNYY244 pKa = 7.38 TSSSNQTTTTLSISNEE260 pKa = 3.39 TRR262 pKa = 11.84 TFDD265 pKa = 3.6 EE266 pKa = 5.08 LNFNLSPTSNSSQAFNYY283 pKa = 10.04 LISDD287 pKa = 3.96 TNVATVNGNTVTIVGVGTTSVTVSQAAGGGYY318 pKa = 7.43 TAATATMTLTINKK331 pKa = 7.15 GTPTISFSNLTKK343 pKa = 10.38 TYY345 pKa = 10.88 GDD347 pKa = 3.8 ANFDD351 pKa = 3.71 LSATSSSTGGLTYY364 pKa = 10.58 SIADD368 pKa = 3.58 ANIASLSVSNVTIVGAGTTSITVTQAADD396 pKa = 3.16 ANYY399 pKa = 10.68 NSATSTITLTVSKK412 pKa = 10.67 LNTTITLSNITKK424 pKa = 10.26 SALDD428 pKa = 3.42 ADD430 pKa = 4.6 FNLDD434 pKa = 4.21 AISSRR439 pKa = 11.84 PGTQVVTSGLVLHH452 pKa = 7.01 LDD454 pKa = 3.69 ASDD457 pKa = 3.37 TDD459 pKa = 4.46 SYY461 pKa = 12.04 DD462 pKa = 3.43 SASGGNTWYY471 pKa = 10.37 DD472 pKa = 2.85 ISGNSNDD479 pKa = 3.03 FTLYY483 pKa = 10.82 GNPTFRR489 pKa = 11.84 SDD491 pKa = 3.55 LNSGTFEE498 pKa = 3.82 FDD500 pKa = 3.18 EE501 pKa = 4.76 NNDD504 pKa = 3.62 YY505 pKa = 11.15 AQSNSTTVLNSTQYY519 pKa = 10.44 TKK521 pKa = 10.6 IAVYY525 pKa = 10.01 YY526 pKa = 9.07 PKK528 pKa = 10.67 SKK530 pKa = 9.68 TKK532 pKa = 10.81 NIVSGGNEE540 pKa = 3.65 AAHH543 pKa = 6.39 AFWMANSTNTIRR555 pKa = 11.84 AGHH558 pKa = 5.22 NTSWSQVSYY567 pKa = 11.38 SPGNMLNSWHH577 pKa = 6.18 YY578 pKa = 10.62 SAVSFSNSDD587 pKa = 3.39 GFKK590 pKa = 10.62 LYY592 pKa = 11.25 YY593 pKa = 10.21 NGNLVDD599 pKa = 5.06 SDD601 pKa = 4.1 NFTNTPNGNSPGLVRR616 pKa = 11.84 IASYY620 pKa = 11.24 NGGNLFDD627 pKa = 4.91 GYY629 pKa = 10.63 IPVVLIYY636 pKa = 10.95 NRR638 pKa = 11.84 VLTSSEE644 pKa = 3.41 IQNNYY649 pKa = 9.38 NNLAQRR655 pKa = 11.84 YY656 pKa = 8.32 GLMNIGNYY664 pKa = 10.52 SNVDD668 pKa = 3.62 LTYY671 pKa = 10.72 SIADD675 pKa = 3.56 TSIATLNGNKK685 pKa = 9.59 VSIVGAGTTTITVSQAADD703 pKa = 3.42 SNFNSGSKK711 pKa = 9.67 TITLTVNTVTPTITFSNEE729 pKa = 2.81 SRR731 pKa = 11.84 TFGDD735 pKa = 3.55 SNFDD739 pKa = 3.62 FSTTSNSSGTFSYY752 pKa = 10.39 IISDD756 pKa = 3.77 TNIATVSGNNVTIRR770 pKa = 11.84 GAGTTSVTVIQAANGIYY787 pKa = 10.02 SSGVATMTLTINKK800 pKa = 9.26 ADD802 pKa = 3.73 PTFSFSDD809 pKa = 3.47 LTKK812 pKa = 10.69 TFGDD816 pKa = 3.87 PNFDD820 pKa = 4.17 LSTSSNSNGIITFTISNTSVARR842 pKa = 11.84 LNPITGGLLLHH853 pKa = 6.58 VDD855 pKa = 3.41 SRR857 pKa = 11.84 DD858 pKa = 3.43 PTSYY862 pKa = 10.02 PGSGNTVYY870 pKa = 10.88 DD871 pKa = 3.9 LSGNNHH877 pKa = 7.15 DD878 pKa = 4.38 FTISGNMNYY887 pKa = 10.7 DD888 pKa = 3.23 NTNGFTFEE896 pKa = 4.19 RR897 pKa = 11.84 GQTAKK902 pKa = 10.77 YY903 pKa = 9.93 LKK905 pKa = 8.73 QTNFPHH911 pKa = 6.48 PTTTITNEE919 pKa = 3.85 ILVKK923 pKa = 10.22 TSSNDD928 pKa = 3.25 NAGLLSYY935 pKa = 11.32 NKK937 pKa = 9.32 TDD939 pKa = 3.46 NDD941 pKa = 3.75 NANLIYY947 pKa = 9.49 ITSGQVRR954 pKa = 11.84 THH956 pKa = 5.98 HH957 pKa = 6.06 TPGFNRR963 pKa = 11.84 NTNVNLSDD971 pKa = 4.02 NQWHH975 pKa = 6.67 HH976 pKa = 6.61 FVQTIDD982 pKa = 3.31 KK983 pKa = 10.65 SSGVDD988 pKa = 3.4 KK989 pKa = 10.77 IYY991 pKa = 11.01 LDD993 pKa = 3.82 GVLVLTYY1000 pKa = 8.92 NTNNTLIPTNGCLAIGQDD1018 pKa = 4.04 FDD1020 pKa = 4.11 TTCGGFNANDD1030 pKa = 3.5 AFGGYY1035 pKa = 9.91 LPLVRR1040 pKa = 11.84 MYY1042 pKa = 11.37 DD1043 pKa = 3.66 RR1044 pKa = 11.84 ILTAEE1049 pKa = 4.22 EE1050 pKa = 3.9 VAQNYY1055 pKa = 9.66 NSLSGATGTGSNTRR1069 pKa = 11.84 IVGAGQTVITFTQAATNNYY1088 pKa = 8.8 NAVTASMTLTVNKK1101 pKa = 8.12 ATPTISISDD1110 pKa = 3.72 EE1111 pKa = 3.5 NRR1113 pKa = 11.84 TFGDD1117 pKa = 3.36 PAFNLSATSSSTGAFTYY1134 pKa = 9.72 TIADD1138 pKa = 3.66 ANVATVIGNTVTIEE1152 pKa = 4.23 GGGTTSVTVTQAADD1166 pKa = 3.42 SNYY1169 pKa = 10.55 SSATSTITLTVNKK1182 pKa = 9.47 FDD1184 pKa = 3.53 PTIIFTGVVTRR1195 pKa = 11.84 TYY1197 pKa = 11.26 NDD1199 pKa = 3.03 SDD1201 pKa = 4.19 FNLSALSSSTASFTYY1216 pKa = 10.36 SIADD1220 pKa = 3.33 ASVATVSGNTVTIVGAGTTTITVSQLANANFNSATASASLIISKK1264 pKa = 10.44 DD1265 pKa = 3.39 NPTLTNFNPIVRR1277 pKa = 11.84 TFGDD1281 pKa = 3.22 ASFEE1285 pKa = 4.12 IVEE1288 pKa = 4.2 PTKK1291 pKa = 11.19 NGDD1294 pKa = 3.31 NTGSFSYY1301 pKa = 10.25 TLLNQNIANISGNTVSITNSGQTSITATLSADD1333 pKa = 3.33 NNYY1336 pKa = 10.49 KK1337 pKa = 10.54 SGTISTSFVVNKK1349 pKa = 9.85 KK1350 pKa = 9.52 NQTITVGALPTTKK1363 pKa = 9.8 PLKK1366 pKa = 10.47 DD1367 pKa = 3.57 FTSIPITASSSSGSPIVISLSAGSAASVSGTVGNYY1402 pKa = 9.55 EE1403 pKa = 4.38 LVSIQQTGLVTITFTTDD1420 pKa = 2.87 DD1421 pKa = 4.29 SGNPNYY1427 pKa = 10.76 NSATTTVVIDD1437 pKa = 3.96 VVKK1440 pKa = 9.71 TNQNISFNTTPTTQLTYY1457 pKa = 10.77 TEE1459 pKa = 4.76 NLDD1462 pKa = 3.62 LTLDD1466 pKa = 3.82 AVASSGLALSYY1477 pKa = 10.97 SIVAGNNATLNNATLSFNDD1496 pKa = 3.48 SGQLTVEE1503 pKa = 4.39 ITQQGNNLYY1512 pKa = 9.41 NQAVPVRR1519 pKa = 11.84 VVFVVGQGNTSLSNFTIPTKK1539 pKa = 10.68 TNLDD1543 pKa = 3.21 NDD1545 pKa = 4.28 FNLTPPTSNRR1555 pKa = 11.84 SGNITYY1561 pKa = 10.47 SSSNLNVATLSGTLISINGIGNTTITATQLANSRR1595 pKa = 11.84 YY1596 pKa = 8.86 KK1597 pKa = 10.45 SASISAVFRR1606 pKa = 11.84 VLVGDD1611 pKa = 3.76 SDD1613 pKa = 4.53 GDD1615 pKa = 4.04 GLIDD1619 pKa = 4.71 SQDD1622 pKa = 3.41 NCPTVANPDD1631 pKa = 3.53 QADD1634 pKa = 3.49 NDD1636 pKa = 3.56 QDD1638 pKa = 4.06 GVGNVCDD1645 pKa = 3.53 NCVDD1649 pKa = 3.83 VNNPLQLDD1657 pKa = 4.23 LDD1659 pKa = 4.15 GDD1661 pKa = 4.69 GYY1663 pKa = 11.58 GDD1665 pKa = 3.58 SCDD1668 pKa = 4.96 AFTLDD1673 pKa = 4.0 ASEE1676 pKa = 4.95 HH1677 pKa = 6.39 ADD1679 pKa = 3.53 SDD1681 pKa = 3.99 GDD1683 pKa = 4.58 GIGDD1687 pKa = 4.38 NADD1690 pKa = 3.46 TDD1692 pKa = 4.28 DD1693 pKa = 6.69 DD1694 pKa = 4.33 NDD1696 pKa = 3.47 NWSDD1700 pKa = 3.82 TIEE1703 pKa = 4.34 IACGTNPRR1711 pKa = 11.84 DD1712 pKa = 4.17 SADD1715 pKa = 3.74 KK1716 pKa = 10.86 PIDD1719 pKa = 3.69 SDD1721 pKa = 3.96 NDD1723 pKa = 3.59 GDD1725 pKa = 4.77 PDD1727 pKa = 5.16 CLDD1730 pKa = 4.67 PDD1732 pKa = 4.94 DD1733 pKa = 6.46 DD1734 pKa = 4.54 NDD1736 pKa = 4.53 GYY1738 pKa = 11.65 LDD1740 pKa = 3.94 TEE1742 pKa = 4.63 DD1743 pKa = 5.81 LFPFDD1748 pKa = 3.68 NQEE1751 pKa = 3.8 WADD1754 pKa = 3.68 NDD1756 pKa = 3.81 LDD1758 pKa = 5.2 GIGDD1762 pKa = 3.82 NADD1765 pKa = 3.44 TDD1767 pKa = 4.27 DD1768 pKa = 6.91 DD1769 pKa = 4.2 NDD1771 pKa = 3.4 QYY1773 pKa = 11.76 LDD1775 pKa = 3.65 QDD1777 pKa = 4.66 EE1778 pKa = 4.98 IDD1780 pKa = 4.53 CLTDD1784 pKa = 3.78 PFDD1787 pKa = 4.4 SASTPDD1793 pKa = 5.51 DD1794 pKa = 3.88 FDD1796 pKa = 5.98 KK1797 pKa = 11.44 DD1798 pKa = 4.76 LIPDD1802 pKa = 5.15 CIDD1805 pKa = 3.96 PNDD1808 pKa = 5.06 DD1809 pKa = 4.3 NDD1811 pKa = 4.17 SCPDD1815 pKa = 3.6 TEE1817 pKa = 6.26 DD1818 pKa = 4.08 EE1819 pKa = 5.09 FPLDD1823 pKa = 4.56 PEE1825 pKa = 4.75 FCQDD1829 pKa = 3.17 TDD1831 pKa = 3.7 GDD1833 pKa = 4.53 GIDD1836 pKa = 4.76 DD1837 pKa = 4.5 RR1838 pKa = 11.84 FDD1840 pKa = 3.97 FDD1842 pKa = 4.95 SDD1844 pKa = 3.38 NDD1846 pKa = 4.81 GIPDD1850 pKa = 3.82 HH1851 pKa = 7.37 RR1852 pKa = 11.84 DD1853 pKa = 3.17 QFPQDD1858 pKa = 3.76 PNASADD1864 pKa = 3.56 GDD1866 pKa = 3.94 GDD1868 pKa = 5.02 GIPDD1872 pKa = 4.54 SQDD1875 pKa = 2.67 TDD1877 pKa = 3.55 KK1878 pKa = 11.79 NNDD1881 pKa = 3.52 GFPDD1885 pKa = 3.63 DD1886 pKa = 4.22 QIIVSSVVTPNQPGIEE1902 pKa = 4.25 EE1903 pKa = 4.03 TWKK1906 pKa = 10.68 VINIEE1911 pKa = 4.32 DD1912 pKa = 3.69 YY1913 pKa = 9.46 PYY1915 pKa = 10.48 TSVRR1919 pKa = 11.84 VYY1921 pKa = 10.88 APDD1924 pKa = 3.62 GSRR1927 pKa = 11.84 VFQSINYY1934 pKa = 8.31 KK1935 pKa = 10.36 NDD1937 pKa = 2.62 WRR1939 pKa = 11.84 GTNIRR1944 pKa = 11.84 TGRR1947 pKa = 11.84 PLPTGPYY1954 pKa = 8.88 YY1955 pKa = 10.97 YY1956 pKa = 10.11 RR1957 pKa = 11.84 IEE1959 pKa = 4.29 LGGTSGEE1966 pKa = 4.78 IIDD1969 pKa = 3.52 GWLYY1973 pKa = 10.67 IFNN1976 pKa = 4.53
Molecular weight: 210.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.795
IPC_protein 3.872
Toseland 3.63
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.859
Rodwell 3.694
Grimsley 3.528
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.317
Thurlkill 3.681
EMBOSS 3.859
Sillero 3.999
Patrickios 1.125
IPC_peptide 3.872
IPC2_peptide 3.973
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A2U2RXD9|A0A2U2RXD9_9BACT Acetolactate synthase OS=Bacteroidetes bacterium SCGC AAA795-G10 OX=2175803 GN=ilvB PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.33 KK3 pKa = 8.37 STKK6 pKa = 9.95 VRR8 pKa = 11.84 IFFEE12 pKa = 4.41 RR13 pKa = 11.84 NGFAVCSRR21 pKa = 11.84 LAEE24 pKa = 4.01 ILGMRR29 pKa = 11.84 VKK31 pKa = 10.36 SVRR34 pKa = 11.84 LFFIYY39 pKa = 10.45 ASFTTLVGGFVLYY52 pKa = 10.79 LILALWLKK60 pKa = 10.61 LNNLIYY66 pKa = 10.12 TKK68 pKa = 10.18 RR69 pKa = 11.84 ASVFDD74 pKa = 3.9 LL75 pKa = 4.15
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.911
IPC_protein 10.526
Toseland 10.73
ProMoST 10.35
Dawson 10.833
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.228
Grimsley 10.877
Solomon 10.906
Lehninger 10.877
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.994
IPC_peptide 10.906
IPC2_peptide 9.385
IPC2.peptide.svr19 8.442
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1767
0
1767
599355
33
5491
339.2
38.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.139 ± 0.057
0.783 ± 0.017
5.445 ± 0.107
6.07 ± 0.066
5.577 ± 0.062
6.498 ± 0.072
1.615 ± 0.031
8.955 ± 0.07
8.531 ± 0.116
9.465 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.012 ± 0.027
6.737 ± 0.066
3.513 ± 0.032
3.096 ± 0.03
3.493 ± 0.039
7.506 ± 0.074
5.065 ± 0.088
5.436 ± 0.04
1.145 ± 0.023
3.918 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here