Arsenophonus endosymbiont of Aleurodicus dispersus
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 381 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B1E2U7|A0A3B1E2U7_9GAMM 50S ribosomal protein L1 OS=Arsenophonus endosymbiont of Aleurodicus dispersus OX=235559 GN=rplA PE=3 SV=1
MM1 pKa = 7.29 VASTLNNPLTYY12 pKa = 10.07 CLLVTEE18 pKa = 4.9 SAYY21 pKa = 9.2 GTQAATNAWQFANTLIMEE39 pKa = 4.88 GYY41 pKa = 9.65 ILNKK45 pKa = 9.81 VFFSQDD51 pKa = 2.29 GVYY54 pKa = 10.72 NGNQLISPASDD65 pKa = 3.08 EE66 pKa = 4.33 FNLLIGWKK74 pKa = 8.27 TLAQQTGCKK83 pKa = 10.32 LNICVAAALRR93 pKa = 11.84 RR94 pKa = 11.84 GIIDD98 pKa = 3.51 ASEE101 pKa = 4.1 AKK103 pKa = 10.82 NNGLPCANLDD113 pKa = 3.52 EE114 pKa = 5.58 DD115 pKa = 4.63 FVLSGLASLAEE126 pKa = 4.11 AMLLCDD132 pKa = 3.92 RR133 pKa = 11.84 TVQFF137 pKa = 4.67
Molecular weight: 14.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.169
IPC2_protein 4.469
IPC_protein 4.304
Toseland 4.126
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.215
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 0.896
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.321
Protein with the highest isoelectric point:
>tr|A0A3B1E9T6|A0A3B1E9T6_9GAMM Pyridoxine 5'-phosphate synthase OS=Arsenophonus endosymbiont of Aleurodicus dispersus OX=235559 GN=pdxJ PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.58 RR12 pKa = 11.84 NCSHH16 pKa = 6.9 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 HH29 pKa = 3.91 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
381
0
381
119123
38
1408
312.7
35.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.35 ± 0.106
1.166 ± 0.04
5.056 ± 0.093
6.122 ± 0.108
3.727 ± 0.078
6.449 ± 0.108
2.334 ± 0.055
8.788 ± 0.117
6.725 ± 0.092
10.174 ± 0.148
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.528 ± 0.054
5.004 ± 0.069
3.647 ± 0.048
4.224 ± 0.092
5.19 ± 0.098
5.687 ± 0.071
5.146 ± 0.06
6.365 ± 0.099
1.035 ± 0.053
3.283 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here