Pacific flying fox faeces associated circular DNA virus-2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTV3|A0A140CTV3_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-2 OX=1796011 PE=3 SV=1
MM1 pKa = 7.86ASPPSRR7 pKa = 11.84QRR9 pKa = 11.84QARR12 pKa = 11.84NIIKK16 pKa = 10.18GLAVCRR22 pKa = 11.84VPPAPEE28 pKa = 3.72TSFQMVGPIRR38 pKa = 11.84QRR40 pKa = 11.84NFRR43 pKa = 11.84INATRR48 pKa = 11.84FLLTYY53 pKa = 8.65PQSTFDD59 pKa = 3.8VQLVWDD65 pKa = 5.11FFNSLAIKK73 pKa = 9.38PKK75 pKa = 10.1RR76 pKa = 11.84AILCRR81 pKa = 11.84EE82 pKa = 3.82HH83 pKa = 7.25HH84 pKa = 6.22QDD86 pKa = 3.04GSEE89 pKa = 4.13HH90 pKa = 4.38VHH92 pKa = 5.35VAIEE96 pKa = 3.84FEE98 pKa = 4.22RR99 pKa = 11.84AVNTTRR105 pKa = 11.84VSIFDD110 pKa = 3.49FGGRR114 pKa = 11.84HH115 pKa = 6.13PNIQSARR122 pKa = 11.84NWAACVNYY130 pKa = 9.52CRR132 pKa = 11.84KK133 pKa = 10.16EE134 pKa = 4.1GVIEE138 pKa = 3.82TAYY141 pKa = 10.45FGCTAEE147 pKa = 4.44DD148 pKa = 3.55ATVASAPGEE157 pKa = 4.04RR158 pKa = 11.84APAEE162 pKa = 4.17SPYY165 pKa = 11.2ARR167 pKa = 11.84AEE169 pKa = 3.9SARR172 pKa = 11.84TIRR175 pKa = 11.84EE176 pKa = 3.71WFEE179 pKa = 3.43YY180 pKa = 10.4CIAEE184 pKa = 4.8GIGYY188 pKa = 10.15AYY190 pKa = 10.54ANAIWNQIHH199 pKa = 5.82SVRR202 pKa = 11.84PPTYY206 pKa = 10.17FEE208 pKa = 4.77NDD210 pKa = 2.77FSGIVSGPTLSHH222 pKa = 6.6LRR224 pKa = 11.84WDD226 pKa = 4.6PEE228 pKa = 3.41WHH230 pKa = 6.71TIFLCGPSGLGKK242 pKa = 10.53SSWALANAPVPFLFVTDD259 pKa = 3.71IDD261 pKa = 4.11DD262 pKa = 5.1LGFFDD267 pKa = 5.39PEE269 pKa = 3.87VHH271 pKa = 6.57KK272 pKa = 11.1ALVFDD277 pKa = 5.39EE278 pKa = 4.47IRR280 pKa = 11.84CTGEE284 pKa = 3.93PNDD287 pKa = 4.15NSAKK291 pKa = 10.43GKK293 pKa = 9.58WPLTSQIKK301 pKa = 9.82LATWDD306 pKa = 3.81TPVSIRR312 pKa = 11.84IRR314 pKa = 11.84YY315 pKa = 8.61KK316 pKa = 9.69IAHH319 pKa = 6.92LPAHH323 pKa = 5.71VPKK326 pKa = 10.59IFTSCDD332 pKa = 3.08WFPMNGDD339 pKa = 3.26EE340 pKa = 5.19QIRR343 pKa = 11.84RR344 pKa = 11.84RR345 pKa = 11.84IKK347 pKa = 10.73AFNLYY352 pKa = 10.16EE353 pKa = 4.72DD354 pKa = 4.13GRR356 pKa = 11.84STEE359 pKa = 4.26SLWLL363 pKa = 3.9

Molecular weight: 41.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTV3|A0A140CTV3_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-2 OX=1796011 PE=3 SV=1
MM1 pKa = 7.86PGQLQRR7 pKa = 11.84YY8 pKa = 8.31RR9 pKa = 11.84RR10 pKa = 11.84GSLALGSAYY19 pKa = 10.52RR20 pKa = 11.84LAKK23 pKa = 10.52AGWKK27 pKa = 9.61AGRR30 pKa = 11.84YY31 pKa = 7.78LAQNINRR38 pKa = 11.84GRR40 pKa = 11.84SMSTLRR46 pKa = 11.84RR47 pKa = 11.84SSGTGGTSRR56 pKa = 11.84RR57 pKa = 11.84GTKK60 pKa = 9.4RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84TSSGGRR69 pKa = 11.84RR70 pKa = 11.84GSYY73 pKa = 10.74QMGSMKK79 pKa = 10.39KK80 pKa = 8.46MRR82 pKa = 11.84GGFSGSRR89 pKa = 11.84DD90 pKa = 3.46GVSSFSTKK98 pKa = 9.56RR99 pKa = 11.84KK100 pKa = 9.79RR101 pKa = 11.84GTKK104 pKa = 10.02RR105 pKa = 11.84GWKK108 pKa = 9.45KK109 pKa = 10.39VLAPQVLRR117 pKa = 11.84TVTSGRR123 pKa = 11.84ILRR126 pKa = 11.84NDD128 pKa = 3.26SGLQTVHH135 pKa = 6.08EE136 pKa = 4.47VLMTNAIPNAVNTTALMSGTTDD158 pKa = 4.56LEE160 pKa = 3.79RR161 pKa = 11.84LYY163 pKa = 11.05QLLRR167 pKa = 11.84VKK169 pKa = 10.68DD170 pKa = 3.92PNTEE174 pKa = 3.86FGQAANATITRR185 pKa = 11.84RR186 pKa = 11.84FFVDD190 pKa = 3.16SCRR193 pKa = 11.84VKK195 pKa = 9.82ITFKK199 pKa = 10.48NQTSIPVEE207 pKa = 3.77LTLYY211 pKa = 10.72DD212 pKa = 3.17ITSRR216 pKa = 11.84KK217 pKa = 10.3DD218 pKa = 3.1NTSALPRR225 pKa = 11.84YY226 pKa = 7.3TPAGSWLEE234 pKa = 3.87GLTNEE239 pKa = 4.39SATFSSEE246 pKa = 4.02PNDD249 pKa = 3.23NAQYY253 pKa = 9.77STFPGSKK260 pKa = 9.18PFEE263 pKa = 4.18SVKK266 pKa = 10.15FCQLFNVRR274 pKa = 11.84KK275 pKa = 8.93SKK277 pKa = 10.71HH278 pKa = 4.92MLLGAGEE285 pKa = 4.15THH287 pKa = 5.62IHH289 pKa = 6.26YY290 pKa = 10.67INLNFKK296 pKa = 10.81YY297 pKa = 9.46MFNRR301 pKa = 11.84EE302 pKa = 4.12EE303 pKa = 3.88FSGFYY308 pKa = 8.8QLRR311 pKa = 11.84NVTQWCLAVLKK322 pKa = 10.65GGIVSSGAAITGPAQVSSSYY342 pKa = 11.21GIVDD346 pKa = 3.58YY347 pKa = 8.41TTEE350 pKa = 3.57AVYY353 pKa = 10.51KK354 pKa = 7.48FKK356 pKa = 11.06CMEE359 pKa = 4.18KK360 pKa = 9.32STTVYY365 pKa = 10.68QSFNALPAIAPAQQTSILEE384 pKa = 4.38DD385 pKa = 3.33TDD387 pKa = 3.73LRR389 pKa = 11.84AVLQTAA395 pKa = 3.89

Molecular weight: 43.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

758

363

395

379.0

42.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.443 ± 0.869

1.715 ± 0.554

3.694 ± 0.717

4.749 ± 0.95

5.145 ± 0.664

7.256 ± 1.266

1.979 ± 0.762

5.277 ± 1.367

4.617 ± 0.951

6.86 ± 0.979

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.715 ± 0.644

4.617 ± 0.152

5.145 ± 1.462

3.826 ± 0.577

8.311 ± 0.433

8.443 ± 1.328

7.52 ± 1.457

5.409 ± 0.073

1.979 ± 0.762

3.298 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski