Capybara microvirus Cap3_SP_437
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W501|A0A4P8W501_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_437 OX=2585452 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.23 VYY4 pKa = 10.79 EE5 pKa = 4.48 GFEE8 pKa = 4.5 KK9 pKa = 10.56 INSFRR14 pKa = 11.84 IVAIPDD20 pKa = 3.46 TKK22 pKa = 10.77 RR23 pKa = 11.84 LFEE26 pKa = 5.7 LYY28 pKa = 9.77 RR29 pKa = 11.84 QSPNLVGNEE38 pKa = 4.43 DD39 pKa = 3.6 EE40 pKa = 5.07 TIDD43 pKa = 4.56 LALEE47 pKa = 4.21 SAQRR51 pKa = 11.84 PTDD54 pKa = 3.44 SMALYY59 pKa = 9.47 EE60 pKa = 4.52 LYY62 pKa = 10.62 SRR64 pKa = 11.84 SIDD67 pKa = 3.75 SEE69 pKa = 4.3
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.62
IPC2_protein 4.609
IPC_protein 4.431
Toseland 4.279
ProMoST 4.444
Dawson 4.368
Bjellqvist 4.596
Wikipedia 4.24
Rodwell 4.266
Grimsley 4.19
Solomon 4.368
Lehninger 4.317
Nozaki 4.482
DTASelect 4.609
Thurlkill 4.291
EMBOSS 4.253
Sillero 4.533
Patrickios 3.948
IPC_peptide 4.368
IPC2_peptide 4.533
IPC2.peptide.svr19 4.471
Protein with the highest isoelectric point:
>tr|A0A4P8W542|A0A4P8W542_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_437 OX=2585452 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.14 EE3 pKa = 3.95 STKK6 pKa = 10.33 TALKK10 pKa = 10.26 EE11 pKa = 3.56 LAKK14 pKa = 10.75 CILTALIAFITAILSSSCGTTRR36 pKa = 11.84 AVVTNKK42 pKa = 10.22 AEE44 pKa = 4.09 QTNTEE49 pKa = 3.99 IKK51 pKa = 9.3 ITTNNPSTISVDD63 pKa = 3.32 PNTTIDD69 pKa = 3.97 LYY71 pKa = 11.48 KK72 pKa = 10.56 KK73 pKa = 10.55
Molecular weight: 7.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.442
IPC2_protein 8.2
IPC_protein 8.287
Toseland 8.916
ProMoST 9.004
Dawson 9.092
Bjellqvist 9.063
Wikipedia 9.107
Rodwell 9.341
Grimsley 8.639
Solomon 9.399
Lehninger 9.414
Nozaki 9.399
DTASelect 8.902
Thurlkill 9.092
EMBOSS 9.341
Sillero 9.297
Patrickios 7.629
IPC_peptide 9.399
IPC2_peptide 7.819
IPC2.peptide.svr19 7.792
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1626
69
635
271.0
31.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.273 ± 1.484
1.415 ± 0.62
6.027 ± 0.605
5.351 ± 1.082
3.875 ± 1.086
4.121 ± 0.473
2.276 ± 0.747
6.581 ± 0.737
6.335 ± 1.464
7.688 ± 0.461
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.337
8.18 ± 0.433
4.059 ± 0.653
4.182 ± 0.683
4.736 ± 0.403
9.102 ± 1.354
5.351 ± 1.494
4.736 ± 0.77
0.677 ± 0.28
6.581 ± 0.903
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here