CRESS associated satellite molecule
Average proteome isoelectric point is 7.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W814|A0A5Q2W814_9VIRU ATP-dependent helicase Rep OS=CRESS associated satellite molecule OX=2656732 PE=3 SV=1
MM1 pKa = 7.26 LRR3 pKa = 11.84 SRR5 pKa = 11.84 NWCFTLNNYY14 pKa = 7.68 DD15 pKa = 3.65 TTEE18 pKa = 3.92 EE19 pKa = 3.9 QALQEE24 pKa = 4.15 VDD26 pKa = 3.82 CKK28 pKa = 11.29 YY29 pKa = 10.22 MIVGRR34 pKa = 11.84 EE35 pKa = 3.97 TGEE38 pKa = 4.15 LTWTHH43 pKa = 6.57 HH44 pKa = 5.53 LQGFIVFKK52 pKa = 10.07 TMKK55 pKa = 10.08 SLNQMKK61 pKa = 9.74 EE62 pKa = 4.07 LNPRR66 pKa = 11.84 AHH68 pKa = 6.65 WEE70 pKa = 4.05 IANGTAEE77 pKa = 4.24 QNIAYY82 pKa = 9.87 CSKK85 pKa = 10.17 QDD87 pKa = 3.95 KK88 pKa = 10.63 IPFIKK93 pKa = 10.37 GVPPKK98 pKa = 9.97 SQKK101 pKa = 10.51 EE102 pKa = 3.82 KK103 pKa = 11.18 GEE105 pKa = 4.15 MNKK108 pKa = 9.45 RR109 pKa = 11.84 RR110 pKa = 11.84 YY111 pKa = 9.62 EE112 pKa = 3.97 EE113 pKa = 3.96 AFIAAKK119 pKa = 9.79 EE120 pKa = 3.98 GRR122 pKa = 11.84 IEE124 pKa = 5.88 DD125 pKa = 3.77 IPKK128 pKa = 10.95 DD129 pKa = 3.49 MLTRR133 pKa = 11.84 HH134 pKa = 5.8 YY135 pKa = 9.82 NTYY138 pKa = 11.07 LKK140 pKa = 10.0 IQRR143 pKa = 11.84 DD144 pKa = 4.26 YY145 pKa = 11.33 QPNPAPLEE153 pKa = 4.2 DD154 pKa = 3.5 TCGIWIYY161 pKa = 10.84 GPPGVGKK168 pKa = 9.47 SHH170 pKa = 6.12 EE171 pKa = 4.32 VCEE174 pKa = 4.76 KK175 pKa = 11.13 YY176 pKa = 10.36 PDD178 pKa = 4.13 AYY180 pKa = 10.8 QKK182 pKa = 11.02 NLNKK186 pKa = 9.53 WWDD189 pKa = 3.78 GYY191 pKa = 10.55 RR192 pKa = 11.84 GHH194 pKa = 7.24 DD195 pKa = 3.81 VVWLDD200 pKa = 4.41 EE201 pKa = 4.3 IAPEE205 pKa = 4.03 HH206 pKa = 6.56 ASWIVPFLKK215 pKa = 10.16 KK216 pKa = 9.13 WACKK220 pKa = 10.07 FPFPAEE226 pKa = 4.13 FKK228 pKa = 10.78 GSQLQIRR235 pKa = 11.84 PKK237 pKa = 10.48 KK238 pKa = 10.76 VIVTSNYY245 pKa = 9.82 SLEE248 pKa = 4.3 DD249 pKa = 3.66 LGLSAVDD256 pKa = 3.48 YY257 pKa = 10.44 DD258 pKa = 3.62 ALARR262 pKa = 11.84 RR263 pKa = 11.84 FSQRR267 pKa = 11.84 KK268 pKa = 8.34 KK269 pKa = 8.76 NARR272 pKa = 11.84 EE273 pKa = 3.87 IKK275 pKa = 10.23 VLQQ278 pKa = 3.73
Molecular weight: 32.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.824
IPC2_protein 8.024
IPC_protein 7.907
Toseland 7.995
ProMoST 8.419
Dawson 8.712
Bjellqvist 8.843
Wikipedia 8.712
Rodwell 8.785
Grimsley 7.922
Solomon 8.829
Lehninger 8.814
Nozaki 8.99
DTASelect 8.653
Thurlkill 8.741
EMBOSS 8.887
Sillero 8.99
Patrickios 4.469
IPC_peptide 8.829
IPC2_peptide 7.6
IPC2.peptide.svr19 7.765
Protein with the highest isoelectric point:
>tr|A0A5Q2W814|A0A5Q2W814_9VIRU ATP-dependent helicase Rep OS=CRESS associated satellite molecule OX=2656732 PE=3 SV=1
MM1 pKa = 7.26 LRR3 pKa = 11.84 SRR5 pKa = 11.84 NWCFTLNNYY14 pKa = 7.68 DD15 pKa = 3.65 TTEE18 pKa = 3.92 EE19 pKa = 3.9 QALQEE24 pKa = 4.15 VDD26 pKa = 3.82 CKK28 pKa = 11.29 YY29 pKa = 10.22 MIVGRR34 pKa = 11.84 EE35 pKa = 3.97 TGEE38 pKa = 4.15 LTWTHH43 pKa = 6.57 HH44 pKa = 5.53 LQGFIVFKK52 pKa = 10.07 TMKK55 pKa = 10.08 SLNQMKK61 pKa = 9.74 EE62 pKa = 4.07 LNPRR66 pKa = 11.84 AHH68 pKa = 6.65 WEE70 pKa = 4.05 IANGTAEE77 pKa = 4.24 QNIAYY82 pKa = 9.87 CSKK85 pKa = 10.17 QDD87 pKa = 3.95 KK88 pKa = 10.63 IPFIKK93 pKa = 10.37 GVPPKK98 pKa = 9.97 SQKK101 pKa = 10.51 EE102 pKa = 3.82 KK103 pKa = 11.18 GEE105 pKa = 4.15 MNKK108 pKa = 9.45 RR109 pKa = 11.84 RR110 pKa = 11.84 YY111 pKa = 9.62 EE112 pKa = 3.97 EE113 pKa = 3.96 AFIAAKK119 pKa = 9.79 EE120 pKa = 3.98 GRR122 pKa = 11.84 IEE124 pKa = 5.88 DD125 pKa = 3.77 IPKK128 pKa = 10.95 DD129 pKa = 3.49 MLTRR133 pKa = 11.84 HH134 pKa = 5.8 YY135 pKa = 9.82 NTYY138 pKa = 11.07 LKK140 pKa = 10.0 IQRR143 pKa = 11.84 DD144 pKa = 4.26 YY145 pKa = 11.33 QPNPAPLEE153 pKa = 4.2 DD154 pKa = 3.5 TCGIWIYY161 pKa = 10.84 GPPGVGKK168 pKa = 9.47 SHH170 pKa = 6.12 EE171 pKa = 4.32 VCEE174 pKa = 4.76 KK175 pKa = 11.13 YY176 pKa = 10.36 PDD178 pKa = 4.13 AYY180 pKa = 10.8 QKK182 pKa = 11.02 NLNKK186 pKa = 9.53 WWDD189 pKa = 3.78 GYY191 pKa = 10.55 RR192 pKa = 11.84 GHH194 pKa = 7.24 DD195 pKa = 3.81 VVWLDD200 pKa = 4.41 EE201 pKa = 4.3 IAPEE205 pKa = 4.03 HH206 pKa = 6.56 ASWIVPFLKK215 pKa = 10.16 KK216 pKa = 9.13 WACKK220 pKa = 10.07 FPFPAEE226 pKa = 4.13 FKK228 pKa = 10.78 GSQLQIRR235 pKa = 11.84 PKK237 pKa = 10.48 KK238 pKa = 10.76 VIVTSNYY245 pKa = 9.82 SLEE248 pKa = 4.3 DD249 pKa = 3.66 LGLSAVDD256 pKa = 3.48 YY257 pKa = 10.44 DD258 pKa = 3.62 ALARR262 pKa = 11.84 RR263 pKa = 11.84 FSQRR267 pKa = 11.84 KK268 pKa = 8.34 KK269 pKa = 8.76 NARR272 pKa = 11.84 EE273 pKa = 3.87 IKK275 pKa = 10.23 VLQQ278 pKa = 3.73
Molecular weight: 32.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.824
IPC2_protein 8.024
IPC_protein 7.907
Toseland 7.995
ProMoST 8.419
Dawson 8.712
Bjellqvist 8.843
Wikipedia 8.712
Rodwell 8.785
Grimsley 7.922
Solomon 8.829
Lehninger 8.814
Nozaki 8.99
DTASelect 8.653
Thurlkill 8.741
EMBOSS 8.887
Sillero 8.99
Patrickios 4.469
IPC_peptide 8.829
IPC2_peptide 7.6
IPC2.peptide.svr19 7.765
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
278
278
278
278.0
32.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.475 ± 0.0
2.158 ± 0.0
5.036 ± 0.0
7.914 ± 0.0
3.597 ± 0.0
5.396 ± 0.0
2.518 ± 0.0
6.115 ± 0.0
9.712 ± 0.0
6.835 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.0
5.036 ± 0.0
5.755 ± 0.0
5.036 ± 0.0
5.396 ± 0.0
3.957 ± 0.0
4.317 ± 0.0
4.676 ± 0.0
3.237 ± 0.0
4.676 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here