Bifidobacterium actinocoloniiforme DSM 22766 
Average proteome isoelectric point is 6.48 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 1484 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A086Z1J3|A0A086Z1J3_9BIFI Phage protein OS=Bifidobacterium actinocoloniiforme DSM 22766 OX=1437605 GN=BACT_1095 PE=4 SV=1 
MM1 pKa = 7.9  LDD3 pKa = 3.34  INKK6 pKa = 9.74  SAQAYY11 pKa = 8.15  KK12 pKa = 10.63  AYY14 pKa = 9.09  LTEE17 pKa = 4.11  EE18 pKa = 4.15  TGVLDD23 pKa = 5.72  GEE25 pKa = 4.89  DD26 pKa = 3.45  EE27 pKa = 4.17  TLLVGFHH34 pKa = 6.79  LGYY37 pKa = 10.19  KK38 pKa = 8.79  QAEE41 pKa = 4.29  SDD43 pKa = 4.14  CALTVDD49 pKa = 5.06  NIDD52 pKa = 3.6  EE53 pKa = 4.41  AAQTIYY59 pKa = 11.05  EE60 pKa = 4.28  SLRR63 pKa = 11.84  DD64 pKa = 3.76  SGQALCNDD72 pKa = 3.52  SWAGEE77 pKa = 4.07  APVAKK82 pKa = 10.08  DD83 pKa = 3.59  AYY85 pKa = 9.75  RR86 pKa = 11.84  QAAEE90 pKa = 3.64  TALRR94 pKa = 11.84  GFLGWQGPNDD104 pKa = 3.99  GVYY107 pKa = 10.41  AAAQGVSNEE116 pKa = 3.91   
 Molecular weight: 12.44 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.802 
IPC2_protein 4.024 
IPC_protein 3.948 
Toseland    3.757 
ProMoST     4.062 
Dawson      3.923 
Bjellqvist  4.139 
Wikipedia   3.846 
Rodwell     3.783 
Grimsley    3.681 
Solomon     3.923 
Lehninger   3.872 
Nozaki      4.05 
DTASelect   4.228 
Thurlkill   3.808 
EMBOSS      3.859 
Sillero     4.075 
Patrickios  1.914 
IPC_peptide 3.923 
IPC2_peptide  4.05 
IPC2.peptide.svr19  3.982 
 Protein with the highest isoelectric point: 
>tr|A0A086YVS4|A0A086YVS4_9BIFI RCC1 repeat-containing protein OS=Bifidobacterium actinocoloniiforme DSM 22766 OX=1437605 GN=BACT_0280 PE=4 SV=1 
MM1 pKa = 7.44  KK2 pKa = 9.6  RR3 pKa = 11.84  TFQPNNRR10 pKa = 11.84  RR11 pKa = 11.84  RR12 pKa = 11.84  HH13 pKa = 4.79  MKK15 pKa = 9.36  HH16 pKa = 5.81  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MRR23 pKa = 11.84  TRR25 pKa = 11.84  SGRR28 pKa = 11.84  ALINRR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 10.1  GRR39 pKa = 11.84  KK40 pKa = 6.41  TLSAA44 pKa = 4.15   
 Molecular weight: 5.36 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.527 
IPC2_protein 11.316 
IPC_protein 12.925 
Toseland    13.086 
ProMoST     13.583 
Dawson      13.086 
Bjellqvist  13.086 
Wikipedia   13.554 
Rodwell     12.705 
Grimsley    13.13 
Solomon     13.583 
Lehninger   13.481 
Nozaki      13.086 
DTASelect   13.086 
Thurlkill   13.086 
EMBOSS      13.583 
Sillero     13.086 
Patrickios  12.427 
IPC_peptide 13.583 
IPC2_peptide  12.574 
IPC2.peptide.svr19  9.238 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        1484 
 
        
        0
 
        
        1484 
         
        526225
 
        29
 
        3129
 
        354.6
 
        38.52
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        11.18 ± 0.078
0.865 ± 0.02
 
        6.2 ± 0.062
5.438 ± 0.069
 
        3.198 ± 0.043
8.721 ± 0.06
 
        1.966 ± 0.027
4.788 ± 0.054
       
        3.712 ± 0.056
9.392 ± 0.078
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.431 ± 0.027
2.74 ± 0.043
 
        5.11 ± 0.048
4.285 ± 0.045
 
        6.557 ± 0.072
6.73 ± 0.056
 
        5.337 ± 0.054
7.356 ± 0.048
       
        1.407 ± 0.028
2.586 ± 0.037
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here