Bifidobacterium actinocoloniiforme DSM 22766
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1484 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A086Z1J3|A0A086Z1J3_9BIFI Phage protein OS=Bifidobacterium actinocoloniiforme DSM 22766 OX=1437605 GN=BACT_1095 PE=4 SV=1
MM1 pKa = 7.9 LDD3 pKa = 3.34 INKK6 pKa = 9.74 SAQAYY11 pKa = 8.15 KK12 pKa = 10.63 AYY14 pKa = 9.09 LTEE17 pKa = 4.11 EE18 pKa = 4.15 TGVLDD23 pKa = 5.72 GEE25 pKa = 4.89 DD26 pKa = 3.45 EE27 pKa = 4.17 TLLVGFHH34 pKa = 6.79 LGYY37 pKa = 10.19 KK38 pKa = 8.79 QAEE41 pKa = 4.29 SDD43 pKa = 4.14 CALTVDD49 pKa = 5.06 NIDD52 pKa = 3.6 EE53 pKa = 4.41 AAQTIYY59 pKa = 11.05 EE60 pKa = 4.28 SLRR63 pKa = 11.84 DD64 pKa = 3.76 SGQALCNDD72 pKa = 3.52 SWAGEE77 pKa = 4.07 APVAKK82 pKa = 10.08 DD83 pKa = 3.59 AYY85 pKa = 9.75 RR86 pKa = 11.84 QAAEE90 pKa = 3.64 TALRR94 pKa = 11.84 GFLGWQGPNDD104 pKa = 3.99 GVYY107 pKa = 10.41 AAAQGVSNEE116 pKa = 3.91
Molecular weight: 12.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.757
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.139
Wikipedia 3.846
Rodwell 3.783
Grimsley 3.681
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.075
Patrickios 1.914
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.982
Protein with the highest isoelectric point:
>tr|A0A086YVS4|A0A086YVS4_9BIFI RCC1 repeat-containing protein OS=Bifidobacterium actinocoloniiforme DSM 22766 OX=1437605 GN=BACT_0280 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.79 MKK15 pKa = 9.36 HH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 6.41 TLSAA44 pKa = 4.15
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1484
0
1484
526225
29
3129
354.6
38.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.18 ± 0.078
0.865 ± 0.02
6.2 ± 0.062
5.438 ± 0.069
3.198 ± 0.043
8.721 ± 0.06
1.966 ± 0.027
4.788 ± 0.054
3.712 ± 0.056
9.392 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.431 ± 0.027
2.74 ± 0.043
5.11 ± 0.048
4.285 ± 0.045
6.557 ± 0.072
6.73 ± 0.056
5.337 ± 0.054
7.356 ± 0.048
1.407 ± 0.028
2.586 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here