Amino acid dipepetide frequency for Bacillus phage Stahl

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.651AlaAla: 4.651 ± 0.587
0.545AlaCys: 0.545 ± 0.158
4.148AlaAsp: 4.148 ± 0.586
4.735AlaGlu: 4.735 ± 0.516
3.017AlaPhe: 3.017 ± 0.462
4.023AlaGly: 4.023 ± 0.594
1.131AlaHis: 1.131 ± 0.222
4.693AlaIle: 4.693 ± 0.458
5.783AlaLys: 5.783 ± 0.685
3.855AlaLeu: 3.855 ± 0.393
1.55AlaMet: 1.55 ± 0.462
3.981AlaAsn: 3.981 ± 0.47
1.425AlaPro: 1.425 ± 0.307
2.556AlaGln: 2.556 ± 0.481
2.221AlaArg: 2.221 ± 0.286
3.981AlaSer: 3.981 ± 0.475
3.897AlaThr: 3.897 ± 0.674
4.232AlaVal: 4.232 ± 0.353
0.503AlaTrp: 0.503 ± 0.13
2.598AlaTyr: 2.598 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
0.419CysAla: 0.419 ± 0.141
0.084CysCys: 0.084 ± 0.063
0.796CysAsp: 0.796 ± 0.201
0.461CysGlu: 0.461 ± 0.155
0.461CysPhe: 0.461 ± 0.137
0.712CysGly: 0.712 ± 0.195
0.168CysHis: 0.168 ± 0.085
0.461CysIle: 0.461 ± 0.129
0.461CysLys: 0.461 ± 0.142
0.629CysLeu: 0.629 ± 0.151
0.084CysMet: 0.084 ± 0.056
0.629CysAsn: 0.629 ± 0.169
0.377CysPro: 0.377 ± 0.129
0.21CysGln: 0.21 ± 0.098
0.293CysArg: 0.293 ± 0.104
0.335CysSer: 0.335 ± 0.144
0.503CysThr: 0.503 ± 0.134
0.503CysVal: 0.503 ± 0.137
0.042CysTrp: 0.042 ± 0.039
0.377CysTyr: 0.377 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
3.059AspAla: 3.059 ± 0.326
0.712AspCys: 0.712 ± 0.185
3.562AspAsp: 3.562 ± 0.35
4.4AspGlu: 4.4 ± 0.575
3.268AspPhe: 3.268 ± 0.358
4.861AspGly: 4.861 ± 0.507
0.754AspHis: 0.754 ± 0.213
4.735AspIle: 4.735 ± 0.418
5.238AspLys: 5.238 ± 0.476
5.196AspLeu: 5.196 ± 0.528
1.76AspMet: 1.76 ± 0.262
3.143AspAsn: 3.143 ± 0.358
1.928AspPro: 1.928 ± 0.248
2.137AspGln: 2.137 ± 0.357
2.053AspArg: 2.053 ± 0.396
3.394AspSer: 3.394 ± 0.461
3.436AspThr: 3.436 ± 0.449
4.525AspVal: 4.525 ± 0.463
0.754AspTrp: 0.754 ± 0.156
2.891AspTyr: 2.891 ± 0.44
0.0AspXaa: 0.0 ± 0.0
Glu
3.939GluAla: 3.939 ± 0.458
0.796GluCys: 0.796 ± 0.194
3.813GluAsp: 3.813 ± 0.421
6.83GluGlu: 6.83 ± 0.671
3.185GluPhe: 3.185 ± 0.425
4.442GluGly: 4.442 ± 0.464
1.299GluHis: 1.299 ± 0.242
4.274GluIle: 4.274 ± 0.466
6.118GluLys: 6.118 ± 0.611
6.285GluLeu: 6.285 ± 0.558
2.975GluMet: 2.975 ± 0.402
4.819GluAsn: 4.819 ± 0.433
1.299GluPro: 1.299 ± 0.241
3.478GluGln: 3.478 ± 0.374
3.31GluArg: 3.31 ± 0.591
3.897GluSer: 3.897 ± 0.356
4.525GluThr: 4.525 ± 0.447
5.112GluVal: 5.112 ± 0.605
1.089GluTrp: 1.089 ± 0.217
2.933GluTyr: 2.933 ± 0.365
0.0GluXaa: 0.0 ± 0.0
Phe
2.682PheAla: 2.682 ± 0.269
0.419PheCys: 0.419 ± 0.11
3.478PheAsp: 3.478 ± 0.343
3.687PheGlu: 3.687 ± 0.354
1.76PhePhe: 1.76 ± 0.301
3.017PheGly: 3.017 ± 0.41
0.461PheHis: 0.461 ± 0.159
2.807PheIle: 2.807 ± 0.43
3.771PheLys: 3.771 ± 0.261
3.31PheLeu: 3.31 ± 0.526
1.089PheMet: 1.089 ± 0.245
3.562PheAsn: 3.562 ± 0.32
1.76PhePro: 1.76 ± 0.316
1.844PheGln: 1.844 ± 0.231
1.718PheArg: 1.718 ± 0.226
2.807PheSer: 2.807 ± 0.369
3.268PheThr: 3.268 ± 0.394
2.43PheVal: 2.43 ± 0.313
0.377PheTrp: 0.377 ± 0.144
1.676PheTyr: 1.676 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
3.52GlyAla: 3.52 ± 0.491
0.377GlyCys: 0.377 ± 0.112
3.436GlyAsp: 3.436 ± 0.345
4.274GlyGlu: 4.274 ± 0.39
3.268GlyPhe: 3.268 ± 0.519
4.358GlyGly: 4.358 ± 0.568
0.838GlyHis: 0.838 ± 0.16
4.903GlyIle: 4.903 ± 0.521
5.447GlyLys: 5.447 ± 0.503
5.405GlyLeu: 5.405 ± 0.567
1.718GlyMet: 1.718 ± 0.289
3.31GlyAsn: 3.31 ± 0.452
0.419GlyPro: 0.419 ± 0.152
3.185GlyGln: 3.185 ± 0.447
2.263GlyArg: 2.263 ± 0.314
4.693GlySer: 4.693 ± 0.459
4.106GlyThr: 4.106 ± 0.439
4.232GlyVal: 4.232 ± 0.423
0.838GlyTrp: 0.838 ± 0.153
3.897GlyTyr: 3.897 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
0.964HisAla: 0.964 ± 0.186
0.042HisCys: 0.042 ± 0.038
1.173HisAsp: 1.173 ± 0.234
1.425HisGlu: 1.425 ± 0.272
1.048HisPhe: 1.048 ± 0.198
0.754HisGly: 0.754 ± 0.203
0.503HisHis: 0.503 ± 0.164
1.383HisIle: 1.383 ± 0.235
1.467HisLys: 1.467 ± 0.294
1.383HisLeu: 1.383 ± 0.29
0.293HisMet: 0.293 ± 0.132
0.712HisAsn: 0.712 ± 0.177
0.461HisPro: 0.461 ± 0.159
0.712HisGln: 0.712 ± 0.162
0.796HisArg: 0.796 ± 0.177
0.712HisSer: 0.712 ± 0.145
1.089HisThr: 1.089 ± 0.21
0.838HisVal: 0.838 ± 0.167
0.126HisTrp: 0.126 ± 0.066
0.88HisTyr: 0.88 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
4.819IleAla: 4.819 ± 0.48
0.754IleCys: 0.754 ± 0.19
5.322IleAsp: 5.322 ± 0.469
5.196IleGlu: 5.196 ± 0.563
2.556IlePhe: 2.556 ± 0.277
4.442IleGly: 4.442 ± 0.377
1.173IleHis: 1.173 ± 0.221
3.897IleIle: 3.897 ± 0.341
5.741IleLys: 5.741 ± 0.438
3.729IleLeu: 3.729 ± 0.309
2.053IleMet: 2.053 ± 0.345
4.358IleAsn: 4.358 ± 0.417
1.928IlePro: 1.928 ± 0.286
2.263IleGln: 2.263 ± 0.279
2.807IleArg: 2.807 ± 0.388
3.855IleSer: 3.855 ± 0.411
4.567IleThr: 4.567 ± 0.407
4.567IleVal: 4.567 ± 0.476
0.629IleTrp: 0.629 ± 0.158
2.347IleTyr: 2.347 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
6.034LysAla: 6.034 ± 0.728
0.67LysCys: 0.67 ± 0.183
4.693LysAsp: 4.693 ± 0.457
7.207LysGlu: 7.207 ± 0.577
3.268LysPhe: 3.268 ± 0.302
4.986LysGly: 4.986 ± 0.374
1.592LysHis: 1.592 ± 0.25
5.196LysIle: 5.196 ± 0.636
8.087LysLys: 8.087 ± 0.82
7.165LysLeu: 7.165 ± 0.575
2.514LysMet: 2.514 ± 0.32
5.699LysAsn: 5.699 ± 0.377
2.807LysPro: 2.807 ± 0.457
2.849LysGln: 2.849 ± 0.391
3.981LysArg: 3.981 ± 0.449
4.316LysSer: 4.316 ± 0.462
5.824LysThr: 5.824 ± 0.426
6.076LysVal: 6.076 ± 0.497
0.88LysTrp: 0.88 ± 0.183
3.143LysTyr: 3.143 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
5.699LeuAla: 5.699 ± 0.591
0.754LeuCys: 0.754 ± 0.226
5.364LeuAsp: 5.364 ± 0.485
6.495LeuGlu: 6.495 ± 0.658
2.598LeuPhe: 2.598 ± 0.326
4.777LeuGly: 4.777 ± 0.447
1.299LeuHis: 1.299 ± 0.204
4.484LeuIle: 4.484 ± 0.488
6.746LeuLys: 6.746 ± 0.634
5.154LeuLeu: 5.154 ± 0.467
2.137LeuMet: 2.137 ± 0.31
4.986LeuAsn: 4.986 ± 0.396
2.095LeuPro: 2.095 ± 0.353
2.724LeuGln: 2.724 ± 0.379
3.143LeuArg: 3.143 ± 0.347
4.777LeuSer: 4.777 ± 0.396
4.777LeuThr: 4.777 ± 0.363
4.651LeuVal: 4.651 ± 0.41
1.089LeuTrp: 1.089 ± 0.256
2.682LeuTyr: 2.682 ± 0.328
0.0LeuXaa: 0.0 ± 0.0
Met
1.55MetAla: 1.55 ± 0.269
0.21MetCys: 0.21 ± 0.099
1.76MetAsp: 1.76 ± 0.289
2.179MetGlu: 2.179 ± 0.352
1.215MetPhe: 1.215 ± 0.243
1.844MetGly: 1.844 ± 0.452
0.335MetHis: 0.335 ± 0.118
2.724MetIle: 2.724 ± 0.361
2.807MetLys: 2.807 ± 0.43
2.179MetLeu: 2.179 ± 0.345
1.048MetMet: 1.048 ± 0.221
1.886MetAsn: 1.886 ± 0.329
0.88MetPro: 0.88 ± 0.212
1.048MetGln: 1.048 ± 0.241
1.006MetArg: 1.006 ± 0.222
2.347MetSer: 2.347 ± 0.28
1.886MetThr: 1.886 ± 0.331
1.634MetVal: 1.634 ± 0.263
0.251MetTrp: 0.251 ± 0.112
0.461MetTyr: 0.461 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.897AsnAla: 3.897 ± 0.506
0.293AsnCys: 0.293 ± 0.104
4.274AsnAsp: 4.274 ± 0.412
4.148AsnGlu: 4.148 ± 0.479
2.556AsnPhe: 2.556 ± 0.385
4.986AsnGly: 4.986 ± 0.424
1.048AsnHis: 1.048 ± 0.227
3.226AsnIle: 3.226 ± 0.409
5.824AsnLys: 5.824 ± 0.544
4.777AsnLeu: 4.777 ± 0.359
1.425AsnMet: 1.425 ± 0.274
3.646AsnAsn: 3.646 ± 0.413
2.556AsnPro: 2.556 ± 0.347
2.095AsnGln: 2.095 ± 0.337
2.891AsnArg: 2.891 ± 0.321
3.31AsnSer: 3.31 ± 0.398
3.855AsnThr: 3.855 ± 0.451
3.729AsnVal: 3.729 ± 0.371
0.587AsnTrp: 0.587 ± 0.162
2.137AsnTyr: 2.137 ± 0.265
0.0AsnXaa: 0.0 ± 0.0
Pro
1.634ProAla: 1.634 ± 0.288
0.126ProCys: 0.126 ± 0.074
1.467ProAsp: 1.467 ± 0.316
1.844ProGlu: 1.844 ± 0.302
1.425ProPhe: 1.425 ± 0.222
1.215ProGly: 1.215 ± 0.231
0.461ProHis: 0.461 ± 0.105
2.388ProIle: 2.388 ± 0.352
2.514ProLys: 2.514 ± 0.367
2.053ProLeu: 2.053 ± 0.318
0.67ProMet: 0.67 ± 0.21
1.55ProAsn: 1.55 ± 0.283
0.754ProPro: 0.754 ± 0.201
0.88ProGln: 0.88 ± 0.203
0.838ProArg: 0.838 ± 0.189
2.011ProSer: 2.011 ± 0.337
2.053ProThr: 2.053 ± 0.297
1.55ProVal: 1.55 ± 0.339
0.084ProTrp: 0.084 ± 0.06
1.089ProTyr: 1.089 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
3.143GlnAla: 3.143 ± 0.72
0.21GlnCys: 0.21 ± 0.101
1.802GlnAsp: 1.802 ± 0.295
2.724GlnGlu: 2.724 ± 0.443
1.802GlnPhe: 1.802 ± 0.406
2.137GlnGly: 2.137 ± 0.296
0.461GlnHis: 0.461 ± 0.11
2.472GlnIle: 2.472 ± 0.478
3.185GlnLys: 3.185 ± 0.359
3.729GlnLeu: 3.729 ± 0.468
1.718GlnMet: 1.718 ± 0.375
2.305GlnAsn: 2.305 ± 0.353
0.922GlnPro: 0.922 ± 0.148
3.059GlnGln: 3.059 ± 0.915
1.802GlnArg: 1.802 ± 0.343
2.347GlnSer: 2.347 ± 0.435
1.76GlnThr: 1.76 ± 0.336
2.598GlnVal: 2.598 ± 0.386
0.293GlnTrp: 0.293 ± 0.093
1.215GlnTyr: 1.215 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
1.592ArgAla: 1.592 ± 0.278
0.21ArgCys: 0.21 ± 0.101
2.221ArgAsp: 2.221 ± 0.32
2.43ArgGlu: 2.43 ± 0.4
2.347ArgPhe: 2.347 ± 0.359
2.137ArgGly: 2.137 ± 0.352
0.629ArgHis: 0.629 ± 0.158
3.478ArgIle: 3.478 ± 0.449
4.609ArgLys: 4.609 ± 0.561
3.185ArgLeu: 3.185 ± 0.45
1.592ArgMet: 1.592 ± 0.324
2.179ArgAsn: 2.179 ± 0.379
1.173ArgPro: 1.173 ± 0.222
1.383ArgGln: 1.383 ± 0.224
1.634ArgArg: 1.634 ± 0.277
1.928ArgSer: 1.928 ± 0.308
2.598ArgThr: 2.598 ± 0.361
2.724ArgVal: 2.724 ± 0.279
0.587ArgTrp: 0.587 ± 0.187
1.215ArgTyr: 1.215 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
3.478SerAla: 3.478 ± 0.453
0.461SerCys: 0.461 ± 0.184
3.226SerAsp: 3.226 ± 0.383
4.065SerGlu: 4.065 ± 0.399
2.933SerPhe: 2.933 ± 0.341
4.735SerGly: 4.735 ± 0.585
1.006SerHis: 1.006 ± 0.224
3.855SerIle: 3.855 ± 0.373
4.19SerLys: 4.19 ± 0.469
4.944SerLeu: 4.944 ± 0.466
2.095SerMet: 2.095 ± 0.334
3.143SerAsn: 3.143 ± 0.335
1.425SerPro: 1.425 ± 0.231
2.64SerGln: 2.64 ± 0.491
1.592SerArg: 1.592 ± 0.198
3.897SerSer: 3.897 ± 0.429
3.981SerThr: 3.981 ± 0.448
3.939SerVal: 3.939 ± 0.425
0.67SerTrp: 0.67 ± 0.188
3.017SerTyr: 3.017 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
5.07ThrAla: 5.07 ± 0.864
0.293ThrCys: 0.293 ± 0.111
3.604ThrAsp: 3.604 ± 0.363
3.729ThrGlu: 3.729 ± 0.322
3.646ThrPhe: 3.646 ± 0.444
4.023ThrGly: 4.023 ± 0.518
1.006ThrHis: 1.006 ± 0.185
3.939ThrIle: 3.939 ± 0.549
4.819ThrLys: 4.819 ± 0.42
5.322ThrLeu: 5.322 ± 0.412
1.383ThrMet: 1.383 ± 0.216
4.065ThrAsn: 4.065 ± 0.329
2.137ThrPro: 2.137 ± 0.244
2.514ThrGln: 2.514 ± 0.404
2.053ThrArg: 2.053 ± 0.317
3.31ThrSer: 3.31 ± 0.455
3.646ThrThr: 3.646 ± 0.515
4.609ThrVal: 4.609 ± 0.443
0.712ThrTrp: 0.712 ± 0.125
2.514ThrTyr: 2.514 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
3.604ValAla: 3.604 ± 0.323
0.419ValCys: 0.419 ± 0.171
4.232ValAsp: 4.232 ± 0.384
4.903ValGlu: 4.903 ± 0.561
3.017ValPhe: 3.017 ± 0.424
3.939ValGly: 3.939 ± 0.454
1.341ValHis: 1.341 ± 0.24
4.819ValIle: 4.819 ± 0.462
6.327ValLys: 6.327 ± 0.465
4.106ValLeu: 4.106 ± 0.327
1.844ValMet: 1.844 ± 0.318
4.065ValAsn: 4.065 ± 0.494
1.76ValPro: 1.76 ± 0.183
2.682ValGln: 2.682 ± 0.31
2.933ValArg: 2.933 ± 0.325
4.232ValSer: 4.232 ± 0.433
3.604ValThr: 3.604 ± 0.341
3.981ValVal: 3.981 ± 0.415
0.754ValTrp: 0.754 ± 0.138
2.766ValTyr: 2.766 ± 0.298
0.0ValXaa: 0.0 ± 0.0
Trp
0.545TrpAla: 0.545 ± 0.153
0.126TrpCys: 0.126 ± 0.077
0.587TrpAsp: 0.587 ± 0.158
0.712TrpGlu: 0.712 ± 0.213
0.587TrpPhe: 0.587 ± 0.177
0.754TrpGly: 0.754 ± 0.171
0.377TrpHis: 0.377 ± 0.131
0.922TrpIle: 0.922 ± 0.191
0.922TrpLys: 0.922 ± 0.171
0.838TrpLeu: 0.838 ± 0.196
0.293TrpMet: 0.293 ± 0.111
0.419TrpAsn: 0.419 ± 0.11
0.126TrpPro: 0.126 ± 0.063
0.251TrpGln: 0.251 ± 0.13
0.754TrpArg: 0.754 ± 0.23
0.838TrpSer: 0.838 ± 0.166
0.587TrpThr: 0.587 ± 0.152
0.503TrpVal: 0.503 ± 0.131
0.084TrpTrp: 0.084 ± 0.06
0.587TrpTyr: 0.587 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.226TyrAla: 3.226 ± 0.438
0.503TyrCys: 0.503 ± 0.17
2.849TyrAsp: 2.849 ± 0.303
2.975TyrGlu: 2.975 ± 0.429
1.969TyrPhe: 1.969 ± 0.25
2.347TyrGly: 2.347 ± 0.231
0.88TyrHis: 0.88 ± 0.222
2.347TyrIle: 2.347 ± 0.348
2.933TyrLys: 2.933 ± 0.379
3.185TyrLeu: 3.185 ± 0.404
0.964TyrMet: 0.964 ± 0.192
2.807TyrAsn: 2.807 ± 0.363
0.419TyrPro: 0.419 ± 0.145
1.173TyrGln: 1.173 ± 0.181
1.76TyrArg: 1.76 ± 0.283
2.305TyrSer: 2.305 ± 0.307
2.43TyrThr: 2.43 ± 0.286
2.891TyrVal: 2.891 ± 0.339
0.461TyrTrp: 0.461 ± 0.144
1.76TyrTyr: 1.76 ± 0.342
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 110 proteins (23866 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski