Caloramator mitchellensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Caloramator

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R3JTK4|A0A0R3JTK4_9CLOT Serine-type D-Ala-D-Ala carboxypeptidase OS=Caloramator mitchellensis OX=908809 GN=dacF_1 PE=3 SV=1
MM1 pKa = 7.29AHH3 pKa = 6.89DD4 pKa = 3.69HH5 pKa = 6.48HH6 pKa = 7.28HH7 pKa = 6.8DD8 pKa = 3.5HH9 pKa = 7.1DD10 pKa = 4.83HH11 pKa = 5.18EE12 pKa = 5.48HH13 pKa = 6.55EE14 pKa = 4.31NVVVLTDD21 pKa = 3.51EE22 pKa = 4.7NGVEE26 pKa = 4.25TEE28 pKa = 4.13FEE30 pKa = 4.59IITSLEE36 pKa = 4.21VEE38 pKa = 4.22DD39 pKa = 3.59KK40 pKa = 10.72LYY42 pKa = 11.16YY43 pKa = 10.23VLYY46 pKa = 10.2PLDD49 pKa = 3.96TDD51 pKa = 4.06DD52 pKa = 6.21DD53 pKa = 4.26EE54 pKa = 6.29AVVLRR59 pKa = 11.84YY60 pKa = 10.3DD61 pKa = 3.5EE62 pKa = 5.23DD63 pKa = 4.19EE64 pKa = 4.43NGEE67 pKa = 4.03GSLAAIEE74 pKa = 4.69DD75 pKa = 3.93DD76 pKa = 4.11EE77 pKa = 5.56EE78 pKa = 4.48FDD80 pKa = 3.69KK81 pKa = 11.36VARR84 pKa = 11.84AYY86 pKa = 9.94EE87 pKa = 3.99EE88 pKa = 4.07WLEE91 pKa = 4.12EE92 pKa = 3.82EE93 pKa = 4.56DD94 pKa = 4.84FEE96 pKa = 5.21EE97 pKa = 5.93FEE99 pKa = 5.67DD100 pKa = 5.26DD101 pKa = 5.25EE102 pKa = 4.71EE103 pKa = 6.12DD104 pKa = 3.61EE105 pKa = 4.54FF106 pKa = 6.51

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R3JRA8|A0A0R3JRA8_9CLOT ComE operon protein 1 OS=Caloramator mitchellensis OX=908809 GN=comEA PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.46KK9 pKa = 8.28RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.78RR21 pKa = 11.84MKK23 pKa = 8.53TRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.01VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.19GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2400

0

2400

720919

29

2839

300.4

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.159 ± 0.051

0.943 ± 0.018

5.529 ± 0.047

7.546 ± 0.058

4.688 ± 0.042

6.475 ± 0.051

1.368 ± 0.018

10.198 ± 0.061

8.808 ± 0.048

9.2 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.021

5.839 ± 0.045

2.975 ± 0.031

2.308 ± 0.022

3.871 ± 0.034

5.589 ± 0.039

4.58 ± 0.033

6.696 ± 0.042

0.626 ± 0.019

4.085 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski