Mesta yellow vein mosaic Bahraich virus-[India:Bahraich:2007]
Average proteome isoelectric point is 7.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B1PAJ0|B1PAJ0_9GEMI AC4 OS=Mesta yellow vein mosaic Bahraich virus-[India:Bahraich:2007] OX=508749 GN=AC4 PE=3 SV=1
MM1 pKa = 7.74 PPRR4 pKa = 11.84 RR5 pKa = 11.84 NGIYY9 pKa = 10.25 SKK11 pKa = 10.68 DD12 pKa = 3.68 YY13 pKa = 9.69 FVTYY17 pKa = 8.32 PKK19 pKa = 10.65 CSLTKK24 pKa = 10.69 EE25 pKa = 4.12 EE26 pKa = 5.36 ALSQLLNLQTPTSKK40 pKa = 10.57 KK41 pKa = 9.41 YY42 pKa = 10.62 IRR44 pKa = 11.84 ICRR47 pKa = 11.84 EE48 pKa = 3.51 LHH50 pKa = 6.09 EE51 pKa = 5.96 DD52 pKa = 3.11 GTPRR56 pKa = 11.84 VRR58 pKa = 11.84 VLIQFEE64 pKa = 4.97 GKK66 pKa = 10.06 FKK68 pKa = 9.6 CQNMRR73 pKa = 11.84 FFDD76 pKa = 4.16 LVSPSWSAHH85 pKa = 4.35 FRR87 pKa = 11.84 PNIQGAKK94 pKa = 9.58 SSSDD98 pKa = 3.06 VKK100 pKa = 11.23 SYY102 pKa = 10.8 IEE104 pKa = 4.18 KK105 pKa = 10.92 DD106 pKa = 3.16 GDD108 pKa = 3.65 ILDD111 pKa = 3.83 WGQFQIDD118 pKa = 3.44 GRR120 pKa = 11.84 SARR123 pKa = 11.84 GGQQTANDD131 pKa = 4.05 AYY133 pKa = 10.69 AAALNAGSKK142 pKa = 10.26 SEE144 pKa = 4.31 AIRR147 pKa = 11.84 VIKK150 pKa = 10.15 EE151 pKa = 3.57 LAPKK155 pKa = 10.6 DD156 pKa = 3.7 FVLQFHH162 pKa = 6.7 NLNANLNRR170 pKa = 11.84 IFQEE174 pKa = 4.12 PPAPYY179 pKa = 9.91 VSPFSSSSFDD189 pKa = 3.41 QVPEE193 pKa = 4.03 EE194 pKa = 4.1 LEE196 pKa = 3.86 EE197 pKa = 4.02 WASEE201 pKa = 4.17 NVVDD205 pKa = 5.24 AAARR209 pKa = 11.84 PLRR212 pKa = 11.84 PQSIVIEE219 pKa = 4.44 GDD221 pKa = 3.12 SRR223 pKa = 11.84 TGKK226 pKa = 8.52 TMWARR231 pKa = 11.84 SLGPHH236 pKa = 6.71 NYY238 pKa = 10.18 LCGHH242 pKa = 7.38 LDD244 pKa = 4.18 LSPKK248 pKa = 10.15 VYY250 pKa = 11.07 SNDD253 pKa = 2.25 AWYY256 pKa = 10.92 NVIDD260 pKa = 5.2 DD261 pKa = 4.41 VDD263 pKa = 3.61 PHH265 pKa = 5.85 YY266 pKa = 11.0 LKK268 pKa = 10.7 HH269 pKa = 6.2 FKK271 pKa = 10.7 EE272 pKa = 4.46 FMGAQRR278 pKa = 11.84 DD279 pKa = 3.81 WQSNTKK285 pKa = 9.6 YY286 pKa = 10.57 GKK288 pKa = 9.12 PVQIKK293 pKa = 10.41 GGIPTIFLCNPGPNASYY310 pKa = 11.2 KK311 pKa = 10.7 EE312 pKa = 3.97 FLDD315 pKa = 3.54 EE316 pKa = 5.43 DD317 pKa = 4.1 KK318 pKa = 11.59 NSALKK323 pKa = 10.43 SWALKK328 pKa = 9.99 NATFVFLTQPLYY340 pKa = 11.2 SGTNQSSTQGSEE352 pKa = 3.74 EE353 pKa = 3.96 AQQEE357 pKa = 4.53 EE358 pKa = 4.95 TSGPP362 pKa = 3.61
Molecular weight: 40.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.044
IPC2_protein 6.046
IPC_protein 6.135
Toseland 6.427
ProMoST 6.517
Dawson 6.44
Bjellqvist 6.415
Wikipedia 6.453
Rodwell 6.427
Grimsley 6.547
Solomon 6.44
Lehninger 6.44
Nozaki 6.693
DTASelect 6.883
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.81
Patrickios 4.24
IPC_peptide 6.453
IPC2_peptide 6.664
IPC2.peptide.svr19 6.713
Protein with the highest isoelectric point:
>tr|B1PAI7|B1PAI7_9GEMI Replication enhancer OS=Mesta yellow vein mosaic Bahraich virus-[India:Bahraich:2007] OX=508749 GN=AC3 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.21 RR4 pKa = 11.84 AADD7 pKa = 3.55 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFGSPYY27 pKa = 7.94 TSRR30 pKa = 11.84 VAAPIVRR37 pKa = 11.84 VTKK40 pKa = 9.91 QQAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.8 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 9.9 VQSFVSTPDD82 pKa = 3.06 VVHH85 pKa = 6.88 IGKK88 pKa = 9.48 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.8 RR104 pKa = 11.84 IGKK107 pKa = 8.54 RR108 pKa = 11.84 FCVKK112 pKa = 9.96 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.57 NSHH172 pKa = 6.59 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 9.17 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.58 ATVTGGQYY191 pKa = 11.09 ASNEE195 pKa = 3.8 HH196 pKa = 6.49 ALVKK200 pKa = 10.69 KK201 pKa = 9.38 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 10.61 APLKK245 pKa = 9.09 IRR247 pKa = 11.84 IYY249 pKa = 10.62 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.545
IPC_protein 9.765
Toseland 10.131
ProMoST 9.853
Dawson 10.335
Bjellqvist 10.043
Wikipedia 10.526
Rodwell 10.687
Grimsley 10.409
Solomon 10.365
Lehninger 10.321
Nozaki 10.145
DTASelect 10.028
Thurlkill 10.189
EMBOSS 10.526
Sillero 10.262
Patrickios 10.218
IPC_peptide 10.365
IPC2_peptide 8.902
IPC2.peptide.svr19 8.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1269
112
362
181.3
20.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.886 ± 0.821
2.522 ± 0.665
4.728 ± 0.372
4.177 ± 0.688
3.94 ± 0.536
5.28 ± 0.32
3.625 ± 0.688
4.728 ± 0.601
5.201 ± 0.791
7.486 ± 1.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.049 ± 0.469
6.462 ± 0.722
5.989 ± 0.41
4.886 ± 0.773
7.171 ± 0.797
8.905 ± 1.279
6.068 ± 0.969
6.304 ± 1.347
1.497 ± 0.142
4.098 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here